In vitro Antimicrobial activity of Acacia nilotica , Ziziphus mauritiana , Bauhinia variegate and Lantana camara against some clinical isolated strains

Plants are potent biochemists; biologically active compounds present in the medicinal plants have always been of great interest to scientists working in this field. Thus, the aim of the current study was to screen the antimicrobial activity of Acacia nilotica, Ziziphus mauritiana, Bauhinia variegate and Lantana camara against some selected clinical isolated strains. Although previous studies have documented the antimicrobial properties of these plants, this work is designed to evaluate the specific antibacterial activity of different extracts of these plants against tested microorganisms, in order to know the best extract against specific microorganisms. In this study the fresh parts (leaves, barks & pods) of the test medicinal plant were collected and methanol, ethanol and ethyl acetate extracts were prepared. Antibacterial susceptibility test was done by using Agar diffusion assay method. Statistical analysis was carried out with SPSS 17.0 Windows version. The results of the current study showed that a total of 8 extracts from 4 different plant species were investigated including pods of ethyl acetate extracts of Lantana camara, which showed the highest antimicrobial activity against tested clinical isolates (Bacillus subtilus 2±0.1mm, Bacillus circulans 2.6±0.2mm, Bacillus sphaericius 2±0.1mm, Staphylococcus aureus 2.5±0.1, and Serratia liquefaciens 2.2±0.1mm), followed by its ethyl acetate extracts of leaves. Bark extracts of four tested medicinal plants possess a lower zone on inhibitory activity as compared to the leaves extracts of these plants. Noticeably no antimicrobial activity was found in the methonolic bark extract of Acacia nilotica against the tested bacteria except Bacillu ciurlans. The results of the present investigation clearly indicate that the antibacterial activity varies with the species of the plants and plant material used. Thus, the study ascertains the value of plants used in ayurveda, which could be of considerable interest to the development of new drugs. Studies are in progress to further evaluate the mechanisms of action of these active test extracts on study organisms associated with certain human diseases.

[1]  A. Mann,et al.  Evaluation of the antimicrobial activities of two Ziziphus species (Ziziphus mauritiana L. and Ziziphus spinachristi L.) on some microbial pathogens , 2010 .

[2]  K. H. Khan,et al.  Biochemical compositions and antibacterial activities of Lantana camara plants with yellow, lavender, red and white flowers , 2009 .

[3]  D. Ganjewala,et al.  Effect of Leaf Positions on Total Phenolics, Flavonoids and Proanthocyanidins Content and Antioxidant Activities in Lantana Camara (L) , 2009 .

[4]  P. Barrow,et al.  Enterobacteriaceae , 2008, Poultry Diseases.

[5]  D. Ganjewala,et al.  Antibacterial activities and phytochemical analysis of different plant parts of Nyctanthes arbor-tristis (Linn.). , 2007 .

[6]  P. Kaushik,et al.  Anticancer and antimutagenic properties of Acacia nilotica (Linn.) on 7,12-dimethylbenz(a)anthracene-induced skin papillomagenesis in Swiss albino mice. , 2006, Asian Pacific journal of cancer prevention : APJCP.

[7]  H. Padh,et al.  Search for antibacterial and antifungal agents from selected Indian medicinal plants. , 2006, Journal of ethnopharmacology.

[8]  R. Verma,et al.  Phytochemical and termiticidal study of Lantana camara var. aculeata leaves. , 2006, Fitoterapia.

[9]  L. D. Di Stasi,et al.  Synergism between plant extract and antimicrobial drugs used on Staphylococcus aureus diseases. , 2006, Memorias do Instituto Oswaldo Cruz.

[10]  F. B. Mello,et al.  Effects of Lantana camara (Verbenaceae) on general reproductive performance and teratology in rats. , 2005, Toxicon : official journal of the International Society on Toxinology.

[11]  F. Şahin,et al.  Antimicrobial activity of aqueous and methanol extracts of Juniperus oxycedrus L. , 2003, Journal of ethnopharmacology.

[12]  A D Russell,et al.  Activity and mechanisms of action of selected biocidal agents on Gram-positive and -negative bacteria. , 2003, Journal of applied microbiology.

[13]  T. Nagao,et al.  Antiproliferative constituents in plants 10. Flavones from the leaves of Lantana montevidensis Briq. and consideration of structure-activity relationship. , 2002, Biological & pharmaceutical bulletin.

[14]  D. Newman,et al.  Natural Product Drug Discovery in the Next Millennium , 2001, Pharmaceutical biology.

[15]  E. Colt.  Inactivation of Antibiotics and the Dissemination of Resistance Genes , 2001 .

[16]  I. A. Ross.  Medicinal Plants of the World: Chemical Constituents, Traditional, and Modern Medicinal Uses , 2014 .

[17]  S. Khalid,et al.  Antiplasmodial activity of selected Sudanese medicinal plants with emphasis on­Acacia nilotica , 1999, Phytotherapy research : PTR.

[18]  M. Iwu,et al.  New Antimicrobials of Plant Origin , 1999 .

[19]  K. Cimanga,et al.  Antiamoebic and phytochemical screening of some Congolese medicinal plants. , 1998, Journal of ethnopharmacology.

[20]  W. Müller,et al.  "In vitro" effect of Egyptian tannin-containing plants and their extracts on the survival of pathogenic bacteria. , 1995, DTW. Deutsche tierarztliche Wochenschrift.

[21]  S. Begum,et al.  Pentacyclic triterpenoids from Lantana camara , 1995 .

[22]  A. Clark,et al.  Discovery and Development of Novel Prototype Antibiotics for Opportunistic Infections Related to Acquired Immunodeficiency Syndrome , 1994 .

[23]  Mitchell L. Cohen.  Epidemiology of Drug Resistance: Implications for a Post—Antimicrobial Era , 1992, Science.

[24]  H. Makkar,et al.  Isolation and partial purification of Lantana (Lantana camara L.) toxins. , 1987, Toxicology letters.

[25]  H. Nikaido,et al.  Molecular basis of bacterial outer membrane permeability. , 1985, Microbiological reviews.

[26]  Joan. Taylor.  DIAGNOSTIC TABLES FOR THE COMMON MEDICAL BACTERIA , 1961, Journal of Hygiene.

[27]  B. Evers.  Indian Medicinal Plants , 1875, The Indian medical gazette.