Kinetic Studies of Paclitaxel Production by Taxus canadensis Cultures in Batch and Semicontinuous with Total Cell Recycle

Suspension cultures of Taxus canadensis were elicited with methyl jasmonate (MJ) under defined headspace ethylene concentrations. Kinetic studies of growth, nutrient consumption, pH variation, and paclitaxel accumulation were conducted in batch cultures and semicontinuous culture with total cell recycle. A dramatic increase of paclitaxel was obtained when the cultures were elicited with 100 μM MJ, but cell growth was thereby arrested. Supplementation of acetyl‐CoA and MJ to the culture proved to be another way to improve paclitaxel yields. Using semicontinuous culture with total cell recycle, paclitaxel accumulation was increased by a factor of 4.0 relative to that in the batch culture during 35 days of cultivation.

[1]  A. Fett-Neto,et al.  Kinetics of taxol production, growth, and nutrient uptake in cell suspensions of Taxus cuspidata , 1994, Biotechnology and bioengineering.

[2]  M. Shuler,et al.  Taxol production in bioreactors: Kinetics of biomass accumulation, nutrient uptake, and taxol production by cell suspensions of Taxus baccata , 1995, Biotechnology and bioengineering.

[3]  Minoru Seki,et al.  Continuous Production of Taxol by Cell Culture of Taxus cuspidata , 1995 .

[4]  J. Linden,et al.  Methyl Jasmonate Induced Production of Taxol in Suspension Cultures of Taxus cuspidata: Ethylene Interaction and Induction Models , 1996, Biotechnology progress.

[5]  Susan C. Roberts,et al.  Kinetics of taxol production and nutrient use in suspension cultures of Taxus cuspidata in shake flasks and a Wilson-type bioreactor. , 1996, Enzyme and microbial technology.

[6]  D. Knorr,et al.  Strategies for the improvement of secondary metabolite production in plant cell cultures , 1995 .

[7]  M. Shuler,et al.  The kinetics of taxoid accumulation in cell suspension cultures of Taxus following elicitation with methyl jasmonate. , 1999, Biotechnology and bioengineering.

[8]  R. Arteca,et al.  Taxus Cell Suspension Cultures: Optimizing Growth and Production of Taxol , 1994 .

[9]  W. Weckwerth,et al.  Biosynthesis of taxol: enzymatic acetylation of 10-deacetylbaccatin-III to baccatin-III in crude extracts from roots of Taxus baccata. , 1996, Biochemical and Biophysical Research Communications - BBRC.

[10]  H. J. G. ten Hoopen,et al.  Effects of hydrodynamic stress on cultured plant cells: A literature survey , 1993 .

[11]  M. Shuler,et al.  Large-scale insect and plant cell culture. , 1994, Current opinion in biotechnology.

[12]  J. Linden,et al.  Gas phase composition effects on suspension cultures of Taxus cuspidata , 1995, Biotechnology and bioengineering.

[13]  J. Linden,et al.  Ethylene and Methyl Jasmonate Interaction and Binding Models For Elicited Biosynthetic Steps of Paclitaxel in Suspension Cultures of Taxus Canadensis , 1999 .

[14]  Eric H. Dunlop,et al.  Effect of fluid shear forces on plant cell suspensions , 1994 .

[15]  J. Zhong,et al.  A quantitative analysis of shear effects on cell suspension and cell culture of perilla frutescens in bioreactors. , 1994, Biotechnology and bioengineering.

[16]  J. Linden,et al.  Shear stress effects on plant cell suspension cultures in a rotating wall vessel bioreactor , 1999, Journal of Industrial Microbiology and Biotechnology.