Fast determination of whole blood glucose with a calorimetric micro-biosensor

Abstract Fast determination of whole blood glucose without any pretreatment was achieved using a calorimetric FIA micro-biosensor. Glucose oxidase was covalently immobilized, together with catalase, onto controlled pore glass beads and packed into a micro-column. By reducing the sample volume down to 1 μ1, a measurement period of about 40 s and an enlarged linear range of 0.5–20 mM glucose were obtained. The correlation coefficient was 0.98 when the micro-biosensor was compared to the Reflolux-S blood glucose analyser. The relative standard deviation for 100 blood samples was 3.7%.