Multiple genotypes of the merozoite surface proteins 1 and 2 in Plasmodium falciparum infections in a hypoendemic area in Iran

In Iran, malaria transmission mainly occurs in south‐eastern regions through both Plasmodium falciparum and P. vivax. The genetic diversity of P. falciparum isolates was analysed in 108 patients attending the regional hospital in Chabahar District, using the molecular markers msp1 and msp2. Multiple genotypes were detected in 87% of patients and the mean numbers of msp1 and msp2 genotypes were 2.51 (95% CI: 2.29–2.73) and 2.61 (95% CI: 2.39–2.83) respectively. Various allelic types of msp1 and msp2 were found, with msp2 3D7/IC type detected in 94% of infections. Plasmodium falciparum infections in south‐east Iran appear to have a higher genetic diversity than expected for an area of low transmission. A situation of higher transmission in this area may be emerging, possibly because of reduced efficacy of first‐line treatments.

[1]  A. Bjorkman,et al.  High prevalence of double Plasmodium falciparum dhfr mutations at codons 108 and 59 in the Sistan-Baluchistan province, Iran. , 2003, The Journal of infectious diseases.

[2]  M. Wasserman,et al.  Genetic diversity of Plasmodium falciparum field samples from an isolated Colombian village. , 2002, The American journal of tropical medicine and hygiene.

[3]  N. D. Djadid,et al.  Detection of malaria parasites by nested PCR in south-eastern, Iran: Evidence of highly mixed infections in Chahbahar district , 2002, Malaria Journal.

[4]  J. M. Rubio,et al.  Genotyping of Plasmodium falciparum infections by PCR: a comparative multicentre study. , 2001, Transactions of the Royal Society of Tropical Medicine and Hygiene.

[5]  J. Duchemin,et al.  Plasmodium falciparum parasites in French Guiana: limited genetic diversity and high selfing rate. , 1999, The American journal of tropical medicine and hygiene.

[6]  W. Jarra,et al.  Biased distribution of msp1 and msp2 allelic variants in Plasmodium falciparum populations in Thailand. , 1999, Transactions of the Royal Society of Tropical Medicine and Hygiene.

[7]  C. Rogier,et al.  The epidemiology of multiple Plasmodium falciparum infections. 5. Variation of Plasmodium falciparum msp1 block 2 and msp2 allele prevalence and of infection complexity in two neighbouring Senegalese villages with different transmission conditions , 1999 .

[8]  C. Rogier,et al.  No influence of age on infection complexity and allelic distribution in Plasmodium falciparum infections in Ndiop, a Senegalese village with seasonal, mesoendemic malaria. , 1998, The American journal of tropical medicine and hygiene.

[9]  A. Katzin,et al.  Allelic diversity at the merozoite surface protein-1 locus of Plasmodium falciparum in clinical isolates from the southwestern Brazilian Amazon. , 1998, The American journal of tropical medicine and hygiene.

[10]  A. Craig,et al.  Limited spatial clustering of individual Plasmodium falciparum alleles in field isolates from coastal Kenya. , 1997, The American journal of tropical medicine and hygiene.

[11]  M. Tanner,et al.  Analysis of multiple Plasmodium falciparum infections in Tanzanian children during the phase III trial of the malaria vaccine SPf66. , 1997, The Journal of infectious diseases.

[12]  J. Lines,et al.  Population structure of Plasmodium falciparum in villages with different malaria endemicity in east Africa. , 1997, The American journal of tropical medicine and hygiene.

[13]  C. Rogier,et al.  Different genetic characteristics of Plasmodium falciparum isolates collected during successive clinical malaria episodes in Senegalese children. , 1996, The American journal of tropical medicine and hygiene.

[14]  J. Charlwood,et al.  Gene flow and cross-mating in Plasmodium falciparum in households in a Tanzanian village , 1995, Parasitology.

[15]  M. Alpers,et al.  Plasmodium falciparum: extensive polymorphism in merozoite surface antigen 2 alleles in an area with endemic malaria in Papua New Guinea. , 1994, Experimental parasitology.

[16]  W. Jarra,et al.  High sensitivity of detection of human malaria parasites by the use of nested polymerase chain reaction. , 1993, Molecular and biochemical parasitology.

[17]  G. Edrissian,et al.  Assessment of the response in vivo and in vitro of Plasmodium falciparum to sulphadoxine-pyrimethamine in the malarious areas of Iran. , 1993, The Journal of tropical medicine and hygiene.

[18]  G. Langsley,et al.  Polymorphism of the alleles of the merozoite surface antigens MSA1 and MSA2 in Plasmodium falciparum wild isolates from Colombia. , 1991, Molecular and biochemical parasitology.

[19]  E. Palmquist,et al.  Malaria control in Iran , 1954 .

[20]  Henry A. Erlich,et al.  The polymerase chain reaction. , 1989, Trends in genetics : TIG.

[21]  F. Mahboudi,et al.  Do mutations in Plasmodium falciparum dihydropteroate synthase and dihydrofolate reductase confer resistance to sulfadoxine-pyrimethamine in Iran? , 2002, Transactions of the Royal Society of Tropical Medicine and Hygiene.

[22]  G. Snounou,et al.  Limited genetic diversity of Plasmodium falciparum in field isolates from Honduras. , 1999, The American journal of tropical medicine and hygiene.

[23]  C. Rogier,et al.  Age-dependent carriage of multiple Plasmodium falciparum merozoite surface antigen-2 alleles in asymptomatic malaria infections. , 1995, American Journal of Tropical Medicine and Hygiene.

[24]  W. Jarra,et al.  Genotyping of Plasmodium falciparum isolates by the polymerase chain reaction and potential uses in epidemiological studies. , 1995, Bulletin of the World Health Organization.

[25]  Purnomo,et al.  Allelic variants of the Plasmodium falciparum merozoite surface antigen 2 (MSA-2) in a geographically restricted area of Irian Jaya. , 1994, Molecular and biochemical parasitology.

[26]  M. Ghorbani,et al.  Imported cases of chloroquine-resistant falciparum malaria in Iran. , 1986, Bulletin de la Societe de pathologie exotique et de ses filiales.

[27]  G. Edrissian,et al.  Preliminary study of the response of Plasmodium falciparum to chloroquine in Sistan and Baluchestan province of Iran. , 1985, Transactions of the Royal Society of Tropical Medicine and Hygiene.

[28]  E. Palmquist,et al.  Malaria in Iran. , 1954, Public health reports.