Antioxidant activity of collagen from skin of parang-parang fish (Chirocentrus dorab) using DPPH and CUPRAC methods

Gross Collagen is fibrous protein that becomes the main component of connective tissues. An alternative source of collagen derived from marine animals as a source of antioxidants is needed. Utilization waste from skin of parang-parang fish (Chirocentrus dorab) as a source of collagen can increase the sale value of waste. The purpose of this research were to extract the collagen from skin of parang-parang fish with acid soluble collagen method include salting out and dialysis, also to test the antioxidant activity of collagen with DPPH and CUPRAC method. Collagen extraction steps consisted of pretreatmented with 0.1 M NaOH for 12 hours, hydrolysed with CH3COOH 0.5 M for 48 hours, salting out with 1 M NaCl for 12 hours, and dialysed with distilled water for 12 hours. Concentration of dissolved protein for 12 hours pretreatment was 0.791 ppm. The yield of collagen was 0.9%. The characteristic of collagen analysed by FTIR spectra showed amide A (3425), B (2924.09), I (1647.21), II (1543.05), and III (1246.02) (cm-1). The yield amino acid of collagen was glycine (26.69%), proline (12.24%), glutamic acid (11.72%), arginine (9.81%), alanine (9.51%). IC50 from DPPH method was 926.25 ppm. The antioxidant capacity of 100 ppm collagen was 104.14 μmol trolox / g extract.

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