论文信息 - Visualization of nanoconstructions with DNA-Aptamers for targeted molecules binding on the surface of screen-printed electrodes

Visualization of nanoconstructions with DNA-Aptamers for targeted molecules binding on the surface of screen-printed electrodes

Nanoconstructions of gold nanoparticles (NPs) obtained via pulsed laser ablation in liquid with DNA-aptamer specific to protein tumor marker were visualized on the surface of screen-printed electrode using scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). AuNPs/aptamer nanoconstuctions distribution on the solid surface was studied. More uniform coverage of the carbon electrode surface with the nanoconstuctions was showed in comparison with DNA-aptamer alone on the golden electrode surface. Targeted binding of the tumor marker molecules with the AuNPs/DNA-aptamer nanoconstuctions was approved.

[1] Kenzo Maehashi,et al. Label-free protein biosensor based on aptamer-modified carbon nanotube field-effect transistors. , 2007, Analytical chemistry.

[2] Mohammad Hasanzadeh,et al. Aptamer-based assay of biomolecules: Recent advances in electro-analytical approach , 2017 .

[3] E. N. Eremina,et al. Selection of DNA aptamers for breast cancer , 2016, Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry.

[4] Noemí de-los-Santos-Álvarez,et al. Aptamers as recognition elements for label-free analytical devices , 2008 .

[5] R Rapini,et al. Electrochemical aptasensors for contaminants detection in food and environment: Recent advances. , 2017, Bioelectrochemistry.

[6] A. A. Modestov,et al. Electrochemical aptasensor for lung cancer-related protein detection in crude blood plasma samples , 2016, Scientific Reports.