Laminar distribution and neuronal targets of GABAergic axon terminals in cat primary auditory cortex (AI)

The form, density, and neuronal targets of presumptive axon terminals (puncta) that were immunoreactive for gamma‐aminobutyric acid (GABA) or its synthesizing enzyme, glutamic acid decarboxylase (GAD), were studied in cat primary auditory cortex (AI) in the light microscope. High‐resolution, plastic‐embedded material and frozen sections were used. The chief results were: (1) There was a three‐tiered numerical distribution of puncta, with the highest density in layer Ia, an intermediate number in layers Ib–IVb, and the lowest concentration in layers V and VI, respectively. (2) Each layer had a particular arrangement: layer I puncta were fine and granular (less than 1 μm), and layer V and VI puncta were mixed in size and predominantly small. (3) The form and density of puncta in every layer were distinctive. (4) Immunonegative neurons received, in general, many more axosomatic puncta than immunopositive cells, with the exception of the large multipolar (presumptive basket) cells, which invariably had many puncta in layers II–VI. (5) The number of puncta on the perikarya of GABAergic neurons was sometines related to the number of puncta in the layer, and in other instances it was independent of the layer. Thus, while layer V had a proportion of GABAergic neurons similar to layer IV, it had only a fraction of the number of puncta: perhaps the intrinsic projections of supragranular GABAergic cells are directed toward layer IV, as those of infragranular GABAergic neurons may be.

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