Improvement of biohydrogen production by Enterobacter cloacae IIT-BT 08 under regulated pH.

The present study investigates the effect of pH and intermediate products formation on biological hydrogen production using Enterobacter cloacae IIT-BT 08. Initial pH was found to have a profound effect on hydrogen production potential, while regulating the pH 6.5 throughout the fermentation was found to increase the cumulative hydrogen production rate and yield significantly. Modified Gompertz equation was used to fit the cumulative hydrogen production curves to obtain the hydrogen production potential P, the hydrogen production rate R and lag phase λ. At regulated pH 6.5, higher H(2) yield (3.1molH(2)mol(-1) glucose), specific hydrogen production potential (798.1mL/g) and specific rate of H(2) production (72.1mLL(-1)h(-1)g(-1)) were obtained. The volatile fatty acid profile showed butyrate, ethanol and acetate as the major end metabolites of fermentation under the operating pH conditions tested; however, their pattern of distribution was pH dependent. At the optimum pH of 6.5, the acetate to butyrate ratio (A/B ratio) was found to be higher than that at any other pH. The study also investigates the effect of sodium ions on biohydrogen production potential. It was also found that sodium ion concentration up to 250mM enhanced the hydrogen production potential; however, any further increase in the metal ion concentration had an inhibitory effect.

[1]  Jo-Shu Chang,et al.  Batch and continuous biohydrogen production from starch hydrolysate by Clostridium species , 2008 .

[2]  Youcai Zhao,et al.  The influence of sodium on biohydrogen production from food waste by anaerobic fermentation , 2009 .

[3]  Bruno Fabiano,et al.  Thermodynamic study and optimization of hydrogen production by Enterobacter aerogenes , 2002 .

[4]  J. Lay,et al.  Biohydrogen production as a function of pH and substrate concentration. , 2001, Environmental science & technology.

[5]  P. Hallenbeck,et al.  Fundamentals of the fermentative production of hydrogen. , 2005, Water science and technology : a journal of the International Association on Water Pollution Research.

[6]  D. Das,et al.  Microbial hydrogen production with Bacillus coagulans IIT-BT S1 isolated from anaerobic sewage sludge. , 2007, Bioresource technology.

[7]  V. Yargeau,et al.  Biohydrogen production from wastewater by Clostridium beijerinckii: Effect of pH and substrate concentration , 2009 .

[8]  Chiu-Yue Lin,et al.  A nutrient formulation for fermentative hydrogen production using anaerobic sewage sludge microflora , 2005 .

[9]  Hong-Wei Hou,et al.  Efficient conversion of wheat straw wastes into biohydrogen gas by cow dung compost. , 2006, Bioresource technology.

[10]  Lawrence Pitt,et al.  Biohydrogen production: prospects and limitations to practical application , 2004 .

[11]  Anish Kumar,et al.  Effect of some environmental parameters on fermentative hydrogen production by Enterobacter cloacae DM11. , 2006, Canadian journal of microbiology.

[12]  J. Lay,et al.  Modeling and optimization of anaerobic digested sludge converting starch to hydrogen , 2000, Biotechnology and bioengineering.

[13]  Debabrata Das,et al.  Enhancement of hydrogen production by Enterobacter cloacae IIT-BT 08. , 2000 .

[14]  J. W. Blackburn,et al.  Biohydrogen from complex carbohydrate wastes as feedstocks—Cellulose degraders from a unique series enrichment , 2009 .

[15]  Hyung-Sool Lee,et al.  Biological hydrogen production: prospects and challenges. , 2010, Trends in biotechnology.

[16]  Qiang Guo,et al.  Bio-hydrogen production from food waste and sewage sludge in the presence of aged refuse excavated from refuse landfill , 2008 .

[17]  John R. Benemann,et al.  Biological hydrogen production , 1995 .

[18]  D. Kell,et al.  On the permeability to weak acids and bases of the cytoplasmic membrane of Clostridium pasteurianum. , 1981, Biochemical and biophysical research communications.

[19]  Hubert Bahl,et al.  Parameters Affecting Solvent Production by Clostridium pasteurianum , 1992, Applied and environmental microbiology.

[20]  W. Yusoff,et al.  Effect of salts addition on hydrogen production by C. acetobutylicum. , 2008, Pakistan journal of biological sciences : PJBS.

[21]  Patrick C. Hallenbeck,et al.  Biological hydrogen production; fundamentals and limiting processes , 2002 .

[22]  B. Min,et al.  Biohydrogen production from wheat straw hydrolysate by dark fermentation using extreme thermophilic mixed culture , 2010, Biotechnology and bioengineering.

[23]  R. H. Wijffels,et al.  Feasibility of biological hydrogen production from biomass for utilization in fuel cells , 2000 .

[24]  B. Kalala,et al.  Biohydrogen production by Enterobacter cloacae and Citrobacter freundii in carrier induced granules , 2008, Biotechnology Letters.

[25]  Debabrata Das,et al.  Amelioration of biohydrogen production by a two-stage fermentation process , 2006 .

[26]  The Effect of pH on Continuous Biohydrogen Production from Swine Wastewater Supplemented with Glucose , 2010, Applied biochemistry and biotechnology.

[27]  Mohd Ali Hassan,et al.  Thermophilic biohydrogen production from palm oil mill effluent (POME) using suspended mixed culture. , 2010 .

[28]  Shigeharu Tanisho,et al.  Fermentative hydrogen evolution by Enterobacter aerogenes strain E.82005 , 1987 .

[29]  Minghua Zhou,et al.  Effect of Sodium Ion Concentration on Hydrogen Production from Sucrose by Anaerobic Hydrogen-producing Granular Sludge , 2006 .

[30]  G. R. Zoutberg,et al.  Glucose fermentation byClostridium butyricum grown under a self generated gas atmosphere in chemostat culture , 1985, Applied Microbiology and Biotechnology.

[31]  Atul Narang,et al.  Bacterial gene regulation in diauxic and non-diauxic growth. , 2006, Journal of theoretical biology.

[32]  Gaosheng Zhang,et al.  Enhanced biohydrogen production from cornstalk wastes with acidification pretreatment by mixed anaerobic cultures , 2007 .

[33]  J. Zeikus,et al.  Regulation of hydrogen metabolism in Butyribacterium methylotrophicum by substrate and pH , 1996, Applied Microbiology and Biotechnology.

[34]  J. Lay,et al.  INFLUENCES OF pH AND MOISTURE CONTENT ON THE METHANE PRODUCTION IN HIGH-SOLIDS SLUDGE DIGESTION , 1997 .

[35]  L. K. Bowles,et al.  Effects of butanol on Clostridium acetobutylicum , 1985, Applied and environmental microbiology.

[36]  F. Loewus.  Improvement in Anthrone Method for Determination of Carbohydrates , 1952 .

[37]  Juan Wei,et al.  Biohydrogen production from starch wastewater and application in fuel cell , 2010 .

[38]  F. Sargent,et al.  Dissecting the roles of Escherichia coli hydrogenases in biohydrogen production. , 2008, FEMS microbiology letters.

[39]  Hang-Sik Shin,et al.  Effect of substrate concentration on hydrogen production and 16S rDNA-based analysis of the microbial community in a continuous fermenter , 2006 .

[40]  Yen-Hui Lin,et al.  Effects of temperature and initial pH on biohydrogen production from food-processing wastewater using anaerobic mixed cultures , 2011, Biodegradation.

[41]  Yinguang Chen,et al.  Mesophilic Biohydrogen Production from Food Waste , 2008, 2008 2nd International Conference on Bioinformatics and Biomedical Engineering.

[42]  G. Antonopoulou,et al.  Biofuels generation from sweet sorghum: fermentative hydrogen production and anaerobic digestion of the remaining biomass. , 2008, Bioresource technology.

[43]  E. Crabbe,et al.  Influence of initial pH on hydrogen production from cheese whey. , 2005, Journal of biotechnology.

[44]  T. Noike,et al.  Effect of iron concentration on hydrogen fermentation. , 2001, Bioresource technology.

[45]  S. M. Raj,et al.  A pilot-scale study of biohydrogen production from distillery effluent using defined bacterial co-culture , 2008 .

[46]  Michael Kornaros,et al.  Biohydrogen and methane production from cheese whey in a two-stage anaerobic process , 2008 .

[47]  Samir Kumar Khanal,et al.  Biological hydrogen production: effects of pH and intermediate products , 2003 .

[48]  Debabrata Das,et al.  Continuous hydrogen production by immobilized Enterobacter cloacae IIT-BT 08 using lignocellulosic materials as solid matrices. , 2001 .

[49]  Narendra Kumar,et al.  Production and purification of α-amylase from hydrogen producing Enterobacter cloacae IIT-BT 08 , 2000 .