Isolation and characterization of two methionine: tRNA ligases from wheat germ.

Two methionine: tRNA ligases (here called ligase A and ligase B) with distinctly different enzymatic and molecular properties were isolated in homogenous form from extracts of raw wheat germ. Both the A and B enzyme are composed of single polypeptide chains of Mr 105000 and 70000 respectively. The smaller molecule (B) has been shown not to be a proteolytic fragment of the larger one (A). The catalytic properties of both the A and B enzymes have been established and the Mg2-dependent capacity to charge six purified methionine-accepting tRNAs have been compared to those of the methionine: tRNA ligases from Escherichia coli and bakers' yeast. The possible reasons for the presence of two methionine: tRNA ligases and their unusual monomeric nature are discussed.

[1]  K. Gopinathan,et al.  Two forms of methionyl-transfer RNA synthetase from Mycobacterium smegmatis. , 1976, Biochemical and biophysical research communications.

[2]  A. Fersht,et al.  Subunit interactions in the methionyl-tRNA synthetase of Bacillus stearothermophilus. , 1976, Biochemistry.

[3]  G. W. Hatfield,et al.  Separation of transfer ribonucleic acid by sepharose chromatography using reverse salt gradients. , 1975, Proceedings of the National Academy of Sciences of the United States of America.

[4]  J. J. Rosa,et al.  The site of covalent attachment in the crystalline osmium-tRNA-fMet isomorphous derivative. , 1974, Biochemistry.

[5]  R. Jakes,et al.  Repeated sequences in methionyl‐tRNA synthetase from E. coli , 1974, FEBS letters.

[6]  B. Hartley,et al.  Repeating sequences in aminoacyl-tRNA synthetases , 1974, Nature.

[7]  C. Samuel,et al.  Initiation of Protein Synthesis by Folate-Sufficient and Folate-Deficient Streptococcus faecalis R: Partial Purification and Properties of Methionyl-Transfer Ribonucleic Acid Synthetase and Methionyl-Transfer Ribonucleic Acid Formyltransferase , 1974, Journal of Bacteriology.

[8]  C. Bruton,et al.  The subunit structure of methionyl‐tRNA synthetase from Escherichia coli , 1974, FEBS letters.

[9]  P. Sigler,et al.  Enzymatic synthesis and crystallographic characterization of an isomorphous derivative of yeast formylatable methionine transfer ribonucleic acid containing iodocytidine. , 1973, Biochemistry.

[10]  E. Schlimme,et al.  Isolation and properties of tRNA nucleotidyl transferase from yeast. , 1971, European journal of biochemistry.

[11]  D. Wallach,et al.  Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. , 1971, Biochemistry.

[12]  D. Cassio,et al.  Modification of methionyl-tRNA synthetase by proteolytic cleavage and properties of the trypsin-modified enzyme. , 1971, European journal of biochemistry.

[13]  J. Leis,et al.  N-formylmethionyl-tRNA of wheat chloroplasts. Its synthesis by a wheat transformylase. , 1971, Biochemistry.

[14]  J. Leis,et al.  Protein chain initiation by methionyl-tRNA. , 1970, Biochemical and biophysical research communications.

[15]  R. W. Holley,et al.  Primary structure of wheat germ phenylalanine transfer RNA. , 1969, Proceedings of the National Academy of Sciences of the United States of America.

[16]  J. Waller,et al.  Studies on Methionyl Transfer RNA Synthetase , 1968 .

[17]  R. Heinrikson,et al.  Purification and properties of methionyl-transfer-ribonucleic acid synthetase from Escherichia coli. , 1967, The Biochemical journal.

[18]  I. Gillam,et al.  The separation of soluble ribonucleic acids on benzoylated diethylaminoethylcellulose. , 1967, Biochemistry.

[19]  J. Brown,et al.  Properties of a methionyl-tRNA synthetase from Sarcina lutea. , 1967, Biochimica et biophysica acta.

[20]  E. Moustafa.  PURIFICATION AND PROPERTIES OF LYSYL- AND METHIONYL-SOLUBLE RIBONUCLEIC ACID SYNTHETASES FROM WHEAT GERM. , 1964, Biochimica et biophysica acta.

[21]  B. Ames,et al.  A method for determining the sedimentation behavior of enzymes: application to protein mixtures. , 1961, The Journal of biological chemistry.

[22]  P. Berg.  Acyl adenylates; the interaction of adenosine triphosphate and L-methionine. , 1956, The Journal of biological chemistry.

[23]  Oliver H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.

[24]  R. Julien,et al.  Methionine‐tRNA‐ligase from wheat germ: Purification and properties , 1975, FEBS letters.

[25]  R. W. Holley.  [77] Isolation of sRNA from intact yeast cells , 1967 .

[26]  G. Cantoni,et al.  Procedures in nucleic acid research , 1966 .

[27]  Ennis Layne,et al.  SPECTROPHOTOMETRIC AND TURBIDIMETRIC METHODS FOR MEASURING PROTEINS , 1957 .