Complexing hemp seed protein with pectin for improved emulsion stability.

Hemp seed protein has the potential to be used in food systems as an emulsifying agent; however, there are still some shortcomings associated with hemp seed protein, such as poor solubility and tendency to aggregate. This study aims to improve the dispersibility of hemp seed protein as an emulsifier by complexing with pectin, driven by electrostatic force. Three protein to pectin ratios were used for complexation, from 1:1, 2:1 to 4:1. The complexation improved the polydispersity of hemp seed protein when dispersed in the aqueous phase. The hemp seed protein displayed multimodal size distribution in water at pH = 3.0 due to aggregation, while the incorporation of pectin helped to diminish those aggregated proteins. When the hemp seed protein was used to stabilize the oil-in-water emulsion, its stabilized emulsion showed promising homogenous droplet size distribution after emulsification. However, during the accelerated storage conditions (55°C), the emulsion stabilized solely by hemp seed protein was subjected to extensive coalescence. From day 0 to 9, the droplet size (d4,3 ) increased by 50 folds from 3.215 to 161.6 µm. In contrast, the hemp seed protein-pectin complex exhibited extraordinary stability during the storage test, where size evolution in all three samples was negligible compared to the emulsion stabilized by hemp seed protein. Rheological characterization suggests that pectin provided physical strength, which may help the emulsion droplets to maintain structural integrity under environmental stress. The underlying mechanism could be associated with the formation of a three-dimensional structure by pectin through bridging adjacent emulsion droplets. PRACTICAL APPLICATION: Hemp seed protein is gaining more and more attention as an emerging plant protein. Recently, hemp seed protein has been explored as an emulsifier, but its stabilized emulsion encounters instability issues during storage. Our study suggests pectin could be used as a co-stabilizer for hemp seed protein emulsions.

[1]  Yanting Shen,et al.  Acylation modification and/or guar gum conjugation enhanced functional properties of pea protein isolate , 2021 .

[2]  Donghong Liu,et al.  Citrus pectin modified by microfluidization and ultrasonication: Improved emulsifying and encapsulation properties , 2020, Ultrasonics sonochemistry.

[3]  Bingcan Chen,et al.  Physicochemical properties and aroma profiles of flaxseed proteins extracted from whole flaxseed and flaxseed meal , 2020 .

[4]  S. O'keefe,et al.  Retention of primary bile salts by dry beans (Phaseolus vulgaris L.) during in vitro digestion: Role of bean components and effect of food processing. , 2020, Food research international.

[5]  R. Saurel,et al.  Hemp (Cannabis sativa L.) Protein Extraction Conditions Affect Extraction Yield and Protein Quality. , 2019, Journal of food science.

[6]  Donghong Liu,et al.  Ultrasonic modification of pectin for enhanced 2-furfurylthiol encapsulation: process optimization and mechanisms. , 2019, Journal of the science of food and agriculture.

[7]  Michael J. Miller,et al.  Co-assembly of nisin and zein in microfluidics for enhanced antilisterial activity in Queso Fresco , 2019, LWT.

[8]  V. Krstonošić,et al.  Emulsifying properties of hemp proteins: Effect of isolation technique , 2019, Food Hydrocolloids.

[9]  Functionality of dairy proteins and vegetable proteins in nutritional supplement powders: a review , 2019 .

[10]  P. Ferranti,et al.  Production, digestibility and allergenicity of hemp (Cannabis sativa L.) protein isolates. , 2019, Food research international.

[11]  Yuanfa Liu,et al.  Correlating emulsion properties to microencapsulation efficacy and nutrients retention in mixed proteins system. , 2019, Food research international.

[12]  Lijun Sun,et al.  Pomegranate peel pectin can be used as an effective emulsifier , 2018, Food Hydrocolloids.

[13]  Y. Xiong,et al.  Heating-Aided pH Shifting Modifies Hemp Seed Protein Structure, Cross-Linking, and Emulsifying Properties. , 2018, Journal of agricultural and food chemistry.

[14]  C. Biliaderis,et al.  Hempseed meal protein isolates prepared by different isolation techniques. Part I. physicochemical properties , 2017, Food Hydrocolloids.

[15]  L. Ragona,et al.  New ACE-Inhibitory Peptides from Hemp Seed (Cannabis sativa L.) Proteins. , 2017, Journal of agricultural and food chemistry.

[16]  Ashok R. Patel,et al.  High internal phase emulsions stabilized solely by whey protein isolate-low methoxyl pectin complexes: effect of pH and polymer concentration. , 2017, Food & function.

[17]  R. Aluko.  Hemp Seed (Cannabis sativa L.) Proteins , 2017 .

[18]  Youngsoo Lee,et al.  Surface modification of zein colloidal particles with sodium caseinate to stabilize oil-in-water pickering emulsion , 2016 .

[19]  Yapeng Fang,et al.  Characterisation of whey protein isolate–gum tragacanth electrostatic interactions in aqueous solutions , 2016 .

[20]  S. Armes,et al.  Giant pH-responsive microgel colloidosomes: preparation, interaction dynamics and stability. , 2016, Soft matter.

[21]  I. Joye,et al.  Effect of Maillard Conjugates on the Physical Stability of Zein Nanoparticles Prepared by Liquid Antisolvent Coprecipitation. , 2015, Journal of agricultural and food chemistry.

[22]  Y. Barenholz,et al.  Celecoxib Encapsulation in β-Casein Micelles: Structure, Interactions, and Conformation. , 2015, Langmuir : the ACS journal of surfaces and colloids.

[23]  D. Mcclements,et al.  Formation of vitamin D nanoemulsion-based delivery systems by spontaneous emulsification: factors affecting particle size and stability. , 2015, Food chemistry.

[24]  D. Mcclements,et al.  Food-grade filled hydrogels for oral delivery of lipophilic active ingredients: Temperature-triggered release microgels , 2015 .

[25]  Xiaoquan Yang,et al.  Fabrication and characterization of antioxidant pickering emulsions stabilized by zein/chitosan complex particles (ZCPs). , 2015, Journal of agricultural and food chemistry.

[26]  R. Aluko,et al.  A comparative study of the structural and functional properties of isolated hemp seed (Cannabis sativa L.) albumin and globulin fractions , 2015 .

[27]  D. Mcclements,et al.  Formation and stabilization of nanoemulsion-based vitamin E delivery systems using natural surfactants: Quillaja saponin and lecithin , 2014 .

[28]  Jian Guo,et al.  Protein-based pickering emulsion and oil gel prepared by complexes of zein colloidal particles and stearate. , 2014, Journal of agricultural and food chemistry.

[29]  Prasad Rasane,et al.  Nutritional advantages of oats and opportunities for its processing as value added foods - a review , 2015, Journal of Food Science and Technology.

[30]  J. Carver,et al.  Invited review: Caseins and the casein micelle: their biological functions, structures, and behavior in foods. , 2013, Journal of dairy science.

[31]  Xiaoquan Yang,et al.  Formation of complex interface and stability of oil-in-water (O/W) emulsion prepared by soy lipophilic protein nanoparticles. , 2013, Journal of agricultural and food chemistry.

[32]  A. Voilley,et al.  Properties of spray-dried food flavours microencapsulated with two-layered membranes: Roles of interfacial interactions and water , 2012 .

[33]  Yanfeng Jiang,et al.  Synergistic effects of whey protein–polysaccharide complexes on the controlled release of lipid‐soluble and water‐soluble vitamins in W1/O/W2 double emulsion systems , 2012 .

[34]  J. Al-Hakkak,et al.  Functional egg white-pectin conjugates prepared by controlled Maillard reaction , 2010 .

[35]  Sanghoon Ko,et al.  Use of whey proteins for encapsulation and controlled delivery applications , 2007 .

[36]  J. Vereijken,et al.  Sunflower proteins: overview of their physicochemical, structural and functional properties , 2007 .

[37]  N. Denkov,et al.  Coalescence stability of emulsions containing globular milk proteins. , 2006, Advances in colloid and interface science.

[38]  G. Ruthel,et al.  Pectin/Zein Beads for Potential Colon-Specific Drug Delivery: Synthesis and in Vitro Evaluation , 2006, Drug delivery.

[39]  J. Callaway.  Hempseed as a nutritional resource: An overview , 2004, Euphytica.

[40]  Kim Hj,et al.  Impact of protein surface denaturation on droplet flocculation in hexadecane oil-in-water emulsions stabilized by β-lactoglobulin , 2002 .

[41]  B. Oomah,et al.  Characteristics of hemp (Cannabis sativa L.) seed oil , 2002 .

[42]  I. Raskin,et al.  The Composition of Hemp Seed Oil and Its Potential as an Important Source of Nutrition , 2000 .

[43]  S. Odani.  Isolation and primary structure of a methionine- and cystine-rich seed protein of Cannabis sativa. , 1998, Bioscience, biotechnology, and biochemistry.

[44]  N. Hettiarachchy,et al.  Spray‐Dried Soy Protein Isolate Solubility, Gelling Characteristics, and Extractable Protein as Affected by Antioxidants , 1995 .