Real-time Lissajous imaging with a low-voltage 2-axis MEMS scanner based on electrothermal actuation.

Laser scanning based on Micro-Electro-Mechanical Systems (MEMS) scanners has become very attractive for biomedical endoscopic imaging, such as confocal microscopy or Optical Coherence Tomography (OCT). These scanners are required to be fast to achieve real-time image reconstruction while working at low actuation voltage to comply with medical standards. In this context, we report a 2-axis Micro-Electro-Mechanical Systems (MEMS) electrothermal micro-scannercapable of imaging large fields of view at high frame rates, e.g. from 10 to 80 frames per second. For this purpose, Lissajous scan parameters are chosen to provide the optimal image quality within the scanner capabilities and the sampling rate limit, resulting from the limited A-scan rate of typical swept-sources used for OCT. Images of 233 px × 203 px and 53 px × 53 px at 10 fps and 61 fps, respectively, are experimentally obtained and demonstrate the potential of this micro-scannerfor high definition and high frame rate endoscopic Lissajous imaging.

[1]  J. M. Khosrofian,et al.  Measurement of a Gaussian laser beam diameter through the direct inversion of knife-edge data. , 1983, Applied optics.

[2]  Ming-Jun Li,et al.  Nonlinear optical endomicroscopy for label-free functional histology in vivo , 2017, Light: Science & Applications.

[3]  Xingde Li,et al.  Forward-viewing resonant fiber-optic scanning endoscope of appropriate scanning speed for 3D OCT imaging , 2010, Optics express.

[4]  Huikai Xie,et al.  Optical coherence tomography endoscopic probe based on a tilted MEMS mirror. , 2016, Biomedical optics express.

[5]  Georgios Sakas,et al.  Trends in medical imaging: from 2D to 3D , 2002, Comput. Graph..

[6]  Johannes F de Boer,et al.  Angiography of the retina and the choroid with phase-resolved OCT using interval-optimized backstitched B-scans. , 2012, Optics express.

[7]  Stelzer Contrast, resolution, pixelation, dynamic range and signal‐to‐noise ratio: fundamental limits to resolution in fluorescence light microscopy , 1998 .

[8]  Nicolas Andreff,et al.  Scanning Micromirror Platform Based on MEMS Technology for Medical Application , 2016, Micromachines.

[9]  E. Cocker,et al.  Fiber-optic fluorescence imaging , 2005, Nature Methods.

[10]  Daniel Y. Kim,et al.  Lissajous Scanning Two-photon Endomicroscope for In vivo Tissue Imaging , 2019, Scientific Reports.

[11]  A. Seifert,et al.  Scanning and Tunable Micro-Optics for Endoscopic Optical Coherence Tomography , 2011, Journal of Microelectromechanical Systems.

[12]  Xingde Li,et al.  Rapid-scanning forward-imaging miniature endoscope for real-time optical coherence tomography. , 2004, Optics letters.

[13]  J. Lygeros,et al.  High-speed multiresolution scanning probe microscopy based on Lissajous scan trajectories , 2012, Nanotechnology.

[14]  Nanguang Chen,et al.  Two-Axis Gimbal-Less Electrothermal Micromirror for Large-Angle Circumferential Scanning , 2009, IEEE Journal of Selected Topics in Quantum Electronics.

[15]  Yuxin Leng,et al.  Scanning all-fiber-optic endomicroscopy system for 3D nonlinear optical imaging of biological tissues. , 2009, Optics express.

[16]  Huikai Xie,et al.  An Electrothermal Tip–Tilt–Piston Micromirror Based on Folded Dual S-Shaped Bimorphs , 2009, Journal of Microelectromechanical Systems.

[17]  Wibool Piyawattanametha,et al.  Two-axis MEMS Scanning Catheter for Ultrahigh Resolution Three-dimensional and En Face Imaging. , 2007, Optics express.

[18]  Ralf Kiesslich,et al.  A guide to multimodal endoscopy imaging for gastrointestinal malignancy — an early indicator , 2017, Nature Reviews Gastroenterology &Hepatology.

[19]  Ki-Hun Jeong,et al.  Microscanners for optical endomicroscopic applications , 2017 .

[20]  Joseph A. Izatt,et al.  Constant linear velocity spiral scanning for near video rate 4D OCT ophthalmic and surgical imaging with isotropic transverse sampling. , 2018, Biomedical optics express.

[21]  Huikai Xie,et al.  A Self-Aligned 45°-Tilted Two-Axis Scanning Micromirror for Side-View Imaging , 2016, Journal of Microelectromechanical Systems.

[22]  K. Jeong,et al.  1.65 mm diameter forward-viewing confocal endomicroscopic catheter using a flip-chip bonded electrothermal MEMS fiber scanner. , 2018, Optics express.

[23]  Martin F. Kraus,et al.  Handheld ultrahigh speed swept source optical coherence tomography instrument using a MEMS scanning mirror. , 2013, Biomedical optics express.

[24]  J. Rolland,et al.  Optimization of galvanometer scanning for optical coherence tomography. , 2015, Applied Optics.

[25]  Huikai Xie,et al.  3D in vivo optical coherence tomography based on a low-voltage, large-scan-range 2D MEMS mirror. , 2010, Optics express.

[26]  Huikai Xie,et al.  A multi-degree-of-freedom micromirror utilizing inverted-series-connected bimorph actuators , 2006 .

[27]  Pilhan Kim,et al.  Frequency selection rule for high definition and high frame rate Lissajous scanning , 2017, Scientific Reports.

[28]  Eero P. Simoncelli,et al.  Image quality assessment: from error visibility to structural similarity , 2004, IEEE Transactions on Image Processing.

[29]  Ki-Hun Jeong,et al.  Lissajous fiber scanning for forward viewing optical endomicroscopy using asymmetric stiffness modulation. , 2014, Optics express.

[30]  Adela Ben-Yakar,et al.  Fast-updating and nonrepeating Lissajous image reconstruction method for capturing increased dynamic information. , 2011, Applied optics.

[31]  C. Gorecki,et al.  Design and Fabrication of a 2-Axis Electrothermal MEMS Micro-Scanner for Optical Coherence Tomography † , 2017, Micromachines.

[32]  Thomas D. Wang,et al.  Visualizing epithelial expression of EGFR in vivo with distal scanning side-viewing confocal endomicroscope , 2016, Scientific Reports.

[33]  Michalina J Gora,et al.  Endoscopic optical coherence tomography: technologies and clinical applications [Invited]. , 2017, Biomedical optics express.

[34]  Xingde Li,et al.  Fiber-optic scanning two-photon fluorescence endoscope. , 2006, Optics letters.

[35]  J. Fujimoto,et al.  Optical Coherence Tomography , 1991 .

[36]  S O Reza Moheimani,et al.  High-speed Lissajous-scan atomic force microscopy: scan pattern planning and control design issues. , 2012, The Review of scientific instruments.

[37]  Pasqualina M. Sarro,et al.  A MEMS Actuator System for an Integrated 3-D Optical Coherent Tomography Scanner , 2018, Journal of Microelectromechanical Systems.

[38]  Zhen Qiu,et al.  MEMS based fiber optical microendoscopes , 2015, Displays.