Effect of the conceptus on uterine prostaglandin-F2alpha and prostaglandin-E2 release and synthesis during the periimplantation period in the pig.

The present study was conducted to evaluate the effect of the conceptus on uterine prostaglandin-F2alpha (PGF2alpha) and prostaglandin-E2 (PGE2) release and the expression of prostaglandin synthase enzymes during the periimplantation period in the pig. A surgically generated model with conceptuses developing in only one of the uterine horns was created. The highest concentration of PGF2alpha and PGE2 was found in the gravid uterine horn, compared with the non-gravid horn and the intact horn of cyclic gilts. Endometrial concentration of both PGs in pregnant gilts was elevated regardless of the conceptus in the uterine horn, whereas only myometrial PGE2 concentration increased during pregnancy. Expression of prostaglandin-E2 synthase (mPGES-1) mRNA in the endometrium was upregulated during the oestrous cycle, while protein expression presented a similar pattern to that of PGE2 concentration in the uterine flushings. Prostaglandin-F2alpha synthase (PGFS) mRNA and protein expression in the endometrium did not differ between pregnancy and oestrous cycle but PGFS mRNA in the myometrium increased during pregnancy both in the gravid and the non-gravid uterine horns. We suggest a local effect of the conceptus on PG release pathways but also a more systemic effect within the whole uterus with regard to PG synthesis and accumulation in uterine tissues.

[1]  M. Bogacki,et al.  The influence of embryo presence on prostaglandins synthesis and prostaglandin E2 and F2α content in corpora lutea during periimplantation period in the pig , 2008, Molecular reproduction and development.

[2]  G. Kotwica,et al.  Secretion of estradiol-17beta by porcine endometrium and myometrium during early pregnancy and luteolysis. , 2008, Theriogenology.

[3]  A. Ziecik,et al.  Differential expression of prostaglandin (PG) synthesis enzymes in conceptus during peri-implantation period and endometrial expression of carbonyl reductase/PG 9-ketoreductase in the pig. , 2007, The Journal of endocrinology.

[4]  B. Petroff,et al.  Expression of enzymes of cyclooxygenase pathway and secretion of prostaglandin E2 and F2alpha by porcine myometrium during luteolysis and early pregnancy. , 2006, Theriogenology.

[5]  I. Bogacka,et al.  The expression of short form of leptin receptor gene during early pregnancy in the pig examined by quantitative real time RT-PCR , 2006 .

[6]  U. DeSilva,et al.  Expression of Porcine Endometrial Prostaglandin Synthase During the Estrous Cycle and Early Pregnancy, and Following Endocrine Disruption of Pregnancy1 , 2006, Biology of reproduction.

[7]  L. Brokken,et al.  Molecular cloning and spatiotemporal expression of prostaglandin F synthase and microsomal prostaglandin E synthase-1 in porcine endometrium. , 2006, Endocrinology.

[8]  S. Stefańczyk-Krzymowska,et al.  Local transfer of prostaglandin E2 into the ovary and its retrograde transfer into the uterus in early pregnant sows , 2005, Experimental physiology.

[9]  M. Kaczmarek,et al.  Influence of estrus synchronization of prepubertal gilts on embryo quality. , 2005, The Journal of reproduction and development.

[10]  P. Chouinard,et al.  Effect of dietary fat sources on systemic and intrauterine synthesis of prostaglandins during early pregnancy in gilts. , 2003, Journal of animal science.

[11]  A. Ziecik,et al.  Primary antisera against selected steroids or proteins and secondary antisera against gamma-globulins--an available tool for studies of reproductive processes. , 2002, Reproductive biology.

[12]  A. Ziecik.  Old, new and the newest concepts of inhibition of luteolysis during early pregnancy in pig. , 2002, Domestic animal endocrinology.

[13]  M. E. Wilson,et al.  Differential expression of cyclooxygenase-2 around the time of elongation in the pig conceptus. , 2002, Animal reproduction science.

[14]  K. Okuda,et al.  Different actions of noradrenaline and nitric oxide on the output of prostaglandins and progesterone in cultured bovine luteal cells. , 2000, Prostaglandins & other lipid mediators.

[15]  D. Pomp,et al.  Ontogeny of elongation and gene expression in the early developing porcine conceptus. , 1997, Biology of reproduction.

[16]  K. Okuda,et al.  Up-regulation of oxytocin receptors in porcine endometrium by adenosine 3',5'-monophosphate. , 1997, Biology of reproduction.

[17]  G. Almond,et al.  Identification and distribution of prostaglandin E receptors on porcine luteal cells. , 1996, Biology of reproduction.

[18]  T. Krzymowski,et al.  Total content of prostaglandin F2 alpha in the endometrium and myometrium from various sections of the porcine uterine horn during the estrous cycle. , 1994, Prostaglandins.

[19]  S. Ford,et al.  Luteal maintenance during early pregnancy in the pig: role for prostaglandin E2. , 1994, Prostaglandins.

[20]  R. Blair,et al.  Studies of uterine secretions and products of primary cultures of endometrial cells in pigs. , 2020, Journal of reproduction and fertility. Supplement.

[21]  G. J. King,et al.  Effect of intrauterine application of oestradiol-17 beta and prostaglandin E-2 on the porcine oestrous cycle and uterine endocrinology. , 1992, Journal of reproduction and fertility.

[22]  Z. Zhang,et al.  Prostaglandin E and F2α secretion by glandular and stromal cellsof the pig endometrium in vitro: Effects of estradiol-17β, progesterone, and day of pregnancy , 1991 .

[23]  R. Blair,et al.  Embryonic steroids and the establishment of pregnancy in pigs. , 2020, Journal of reproduction and fertility. Supplement.

[24]  J. Kotwica,et al.  Uterine and ovarian countercurrent pathways in the control of ovarian function in the pig. , 2020, Journal of reproduction and fertility. Supplement.

[25]  J. Ottobre,et al.  Luteal production of steroids and prostaglandins during simulated early pregnancy in the primate: differential regulation of steroid production by chorionic gonadotropin. , 1989, Biology of reproduction.

[26]  J. Diehl,et al.  Prostaglandin E2 opunteracts the effects of PGF2α in indomethacin treated cycling gilts , 1988 .

[27]  R. Kelly,et al.  Production of steroids and release of prostaglandins by spherical pig blastocysts in vitro. , 1986, Australian journal of biological sciences.

[28]  F. Bazer,et al.  Steroid metabolism by endometrial and conceptus tissues during early pregnancy and pseudopregnancy in gilts. , 1985, Journal of reproduction and fertility.

[29]  R. Seamark,et al.  Steroid hormones in uterine washings and in plasma of gilts between days 9 and 15 after oestrus and between days 9 and 15 after coitus. , 1985, Journal of reproduction and fertility.

[30]  P. Pakrasi,et al.  Prostaglandins in swine blastocysts. , 1983, Biology of reproduction.

[31]  R. Roberts,et al.  Establishment of pregnancy in the pig: I. Interrelationships between preimplantation development of the pig blastocyst and uterine endometrial secretions. , 1982, Biology of reproduction.

[32]  R. Hunter,et al.  Uterine secretion of prostaglandin F2 alpha in anaesthetized pigs during the oestrous cycle and early pregnancy. , 1982, Reproduction, nutrition, developpement.

[33]  S. Ford,et al.  Uterine blood flow and uterine arterial, venous and luminal concentrations of oestrogens on days 11, 13 and 15 after oestrus in pregnant and non-pregnant sows. , 1982, Journal of reproduction and fertility.

[34]  C. J. Wilcox,et al.  A study of prostaglandin F2 alpha as the luteolysin in swine: V. Comparison of prostaglandin F, progestins, estrone and estradiol in uterine flushings from pregnant and nonpregnant gilts. , 1980, Prostaglandins.

[35]  C. J. Wilcox,et al.  A study of prostaglandin F2alpha as the luteolysin in swine: III effects of estradiol valerate on prostaglandin F, progestins, estrone and estradiol concentrations in the utero-ovarian vein of nonpregnant gilts. , 1977, Prostaglandins.

[36]  C. J. Wilcox,et al.  A study of prostaglandin F2alpha as the luteolysin in swine: II Characterization and comparison of prostaglandin F, estrogens and progestin concentrations in utero-ovarian vein plasma of nonpregnant and pregnant gilts. , 1977, Prostaglandins.

[37]  F. Bazer,et al.  Theory of maternal recognition of pregnancy in swine based on estrogen controlled endocrine versus exocrine secretion of prostaglandin F2alpha by the uterine endometrium. , 1977, Prostaglandins.

[38]  M. M. Bradford.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.

[39]  E. Knobil,et al.  Time course of serum estrogen and luteinizing hormone (LH) concentrations during the menstrual cycle of the rhesus monkey. , 1971, Endocrinology.