Structure and function of the selectin ligand PSGL-1.

P-selectin glycoprotein ligand-1 (PSGL-1) is a dimeric mucin-like 120-kDa glycoprotein on leukocyte surfaces that binds to P- and L-selectin and promotes cell adhesion in the inflammatory response. The extreme amino terminal extracellular domain of PSGL-1 is critical for these interactions, based on site-directed mutagenesis, blocking monoclonal antibodies, and biochemical analyses. The current hypothesis is that for high affinity interactions with P-selectin, PSGL-1 must contain O-glycans with a core-2 branched motif containing the sialyl Lewis x antigen (NeuAc alpha 2-->3Gal beta 1-->4[Fuc alpha 1-->3]GlcNAc beta 1-->R). In addition, high affinity interactions require the co-expression of tyrosine sulfate on tyrosine residues near the critical O-glycan structure. This review addresses the biochemical evidence for this hypothesis and the evidence that PSGL-1 is an important in vivo ligand for cell adhesion.

[1]  G. Gaetano,et al.  Platelet/Polymorphonuclear Leukocyte Interaction: P-Selectin Triggers Protein-Tyrosine Phosphorylation–Dependent CD11b/CD18 Adhesion: Role of PSGL-1 as a Signaling Molecule , 1999 .

[2]  B. Furie,et al.  The Biology of P-Selectin Glycoprotein Ligand-1: Its Role as a Selectin Counterreceptor in Leukocyte-Endothelial and Leukocyte-Platelet Interaction , 1999, Thrombosis and Haemostasis.

[3]  Richard D. Cummings,et al.  Affinity and Kinetic Analysis of P-selectin Binding to P-selectin Glycoprotein Ligand-1* , 1998, The Journal of Biological Chemistry.

[4]  S. Tsuboi,et al.  Core 2 oligosaccharide biosynthesis distinguishes between selectin ligands essential for leukocyte homing and inflammation. , 1998, Immunity.

[5]  F. Wolber,et al.  Alpha(1,3)-fucosyltransferase VII-dependent synthesis of P- and E-selectin ligands on cultured T lymphoblasts. , 1998, Journal of immunology.

[6]  K. Croce,et al.  Interaction between soluble P-selectin and soluble P-selectin glycoprotein ligand 1: equilibrium binding analysis. , 1998, Biochemistry.

[7]  G S Kansas,et al.  Important contributions of P-selectin glycoprotein ligand-1-mediated secondary capture to human monocyte adhesion to P-selectin, E-selectin, and TNF-alpha-activated endothelium under flow in vitro. , 1998, Journal of immunology.

[8]  R. Nelson,et al.  Dimerization of P-Selectin Glycoprotein Ligand-1 (PSGL-1) Required for Optimal Recognition of P-Selectin , 1998, The Journal of cell biology.

[9]  R. Hynes,et al.  The combined role of P- and E-selectins in atherosclerosis. , 1998, The Journal of clinical investigation.

[10]  R. Busuttil,et al.  Reduction of hepatic ischemia/reperfusion injury by a soluble P-selectin glycoprotein ligand-1. , 1998, Annals of surgery.

[11]  R. Cummings,et al.  Identification of N-terminal Residues on P-selectin Glycoprotein Ligand-1 Required for Binding to P-selectin* , 1998, The Journal of Biological Chemistry.

[12]  K. Moore,et al.  Structure and function of P-selectin glycoprotein ligand-1. , 1998, Leukemia & lymphoma.

[13]  H. Maaheimo,et al.  Complementary Acceptor and Site Specificities of Fuc-TIV and Fuc-TVII Allow Effective Biosynthesis of Sialyl-TriLex and Related Polylactosamines Present on Glycoprotein Counterreceptors of Selectins* , 1998, The Journal of Biological Chemistry.

[14]  K. Ley,et al.  Functional characterization of L-selectin ligands on human neutrophils and leukemia cell lines: evidence for mucinlike ligand activity distinct from P-selectin glycoprotein ligand-1. , 1998, Blood.

[15]  A. Barclay,et al.  Affinity and Kinetic Analysis of L-selectin (CD62L) Binding to Glycosylation-dependent Cell-adhesion Molecule-1* , 1998, The Journal of Biological Chemistry.

[16]  R. Cummings,et al.  Role of PSGL-1 binding to selectins in leukocyte recruitment. , 1997, The Journal of clinical investigation.

[17]  A. Weyrich,et al.  Engagement of P-selectin Glycoprotein Ligand-1 Enhances Tyrosine Phosphorylation and Activates Mitogen-activated Protein Kinases in Human Neutrophils* , 1997, The Journal of Biological Chemistry.

[18]  K. Patel,et al.  Comparison of tethering and rolling of eosinophils and neutrophils through selectins and P-selectin glycoprotein ligand-1. , 1997, Journal of immunology.

[19]  K. Ley,et al.  The P-selectin glycoprotein ligand-1 is important for recruitment of neutrophils into inflamed mouse peritoneum. , 1997, Blood.

[20]  R. Cummings,et al.  Perspectives series: cell adhesion in vascular biology. Role of PSGL-1 binding to selectins in leukocyte recruitment. , 1997, The Journal of clinical investigation.

[21]  J. Lowe.  Selectin ligands, leukocyte trafficking, and fucosyltransferase genes. , 1997, Kidney international.

[22]  K. Comess,et al.  Isolated P-selectin Glycoprotein Ligand-1 Dynamic Adhesion to P- and E-selectin , 1997, The Journal of cell biology.

[23]  D. Vestweber,et al.  P-Selectin Glycoprotein Ligand-1 (PSGL-1) on T Helper 1 but Not on T Helper 2 Cells Binds to P-Selectin and Supports Migration into Inflamed Skin , 1997, Journal of Experimental Medicine.

[24]  D. Cumming,et al.  Core2 beta-1,6-N-acetylglucosaminyltransferase enzyme activity is critical for P-selectin glycoprotein ligand-1 binding to P-selectin. , 1996, Blood.

[25]  M. Schapira,et al.  P-selectin glycoprotein ligand 1 is a ligand for L-selectin on neutrophils, monocytes, and CD34+ hematopoietic progenitor cells , 1996, The Journal of cell biology.

[26]  A. Kuramoto,et al.  Expression and functional characterization of the P-selectin glycoprotein ligand-1 in various cells. , 1996, International journal of hematology.

[27]  L. Sklar,et al.  P-selectin glycoprotein ligand-1 (PSGL-1) is a ligand for L-selectin in neutrophil aggregation. , 1996, Blood.

[28]  T. Kishimoto,et al.  Neutrophil-neutrophil interactions under hydrodynamic shear stress involve L-selectin and PSGL-1. A mechanism that amplifies initial leukocyte accumulation of P-selectin in vitro. , 1996, The Journal of clinical investigation.

[29]  Petr Malý,et al.  The α(1,3)Fucosyltransferase Fuc-TVII Controls Leukocyte Trafficking through an Essential Role in L-, E-, and P-selectin Ligand Biosynthesis , 1996, Cell.

[30]  R. Cummings,et al.  Structures of the O-Glycans on P-selectin Glycoprotein Ligand-1 from HL-60 Cells* , 1996, The Journal of Biological Chemistry.

[31]  C. Kozak,et al.  Mouse P-selectin glycoprotein ligand-1: molecular cloning, chromosomal localization, and expression of a functional P-selectin receptor. , 1996, Blood.

[32]  R. Cummings,et al.  Visualization of P-selectin Glycoprotein Ligand-1 as a Highly Extended Molecule and Mapping of Protein Epitopes for Monoclonal Antibodies (*) , 1996, The Journal of Biological Chemistry.

[33]  Richard D. Cummings,et al.  Post-translational Modifications of Recombinant P-selectin Glycoprotein Ligand-1 Required for Binding to P- and E-selectin (*) , 1996, The Journal of Biological Chemistry.

[34]  K E Norman,et al.  Leukocyte rolling in vivo is mediated by P-selectin glycoprotein ligand-1. , 1995, Blood.

[35]  D. Cumming,et al.  A Novel Cobra Venom Metalloproteinase, Mocarhagin, Cleaves a 10-Amino Acid Peptide from the Mature N Terminus of P-selectin Glycoprotein Ligand Receptor, PSGL-1, and Abolishes P-selectin Binding (*) , 1995, The Journal of Biological Chemistry.

[36]  Brian Seed,et al.  PSGL-1 recognition of P-selectin is controlled by a tyrosine sulfation consensus at the PSGL-1 amino terminus , 1995, Cell.

[37]  K. Comess,et al.  A sulfated peptide segment at the amino terminus of PSGL-1 is critical for P-selectin binding , 1995, Cell.

[38]  M. U. Nollert,et al.  Neutrophils use both shared and distinct mechanisms to adhere to selectins under static and flow conditions. , 1995, The Journal of clinical investigation.

[39]  Richard D. Cummings,et al.  Tyrosine Sulfation of P-selectin Glycoprotein Ligand-1 Is Required for High Affinity Binding to P-selectin (*) , 1995, The Journal of Biological Chemistry.

[40]  R. Eddy,et al.  Genomic Organization and Chromosomal Localization of the Gene Encoding Human P-selectin Glycoprotein Ligand (*) , 1995, The Journal of Biological Chemistry.

[41]  G. Zimmerman,et al.  Activation of polymorphonuclear leukocytes reduces their adhesion to P-selectin and causes redistribution of ligands for P-selectin on their surfaces. , 1995, The Journal of clinical investigation.

[42]  R. Cummings,et al.  P-selectin glycoprotein ligand-1 mediates rolling of human neutrophils on P-selectin , 1995, The Journal of cell biology.

[43]  R. Cummings,et al.  The P-selectin glycoprotein ligand from human neutrophils displays sialylated, fucosylated, O-linked poly-N-acetyllactosamine. , 1994, The Journal of biological chemistry.

[44]  B. Furie,et al.  Expression cloning of a functional glycoprotein ligand for P-selectin , 1993, Cell.

[45]  R. Cummings,et al.  Characterization of a specific ligand for P-selectin on myeloid cells. A minor glycoprotein with sialylated O-linked oligosaccharides. , 1993, The Journal of biological chemistry.

[46]  R. Cummings,et al.  Identification of a specific glycoprotein ligand for P-selectin (CD62) on myeloid cells , 1992, The Journal of cell biology.

[47]  K. Handa,et al.  Inhibition of selectin-dependent tumor cell adhesion to endothelial cells and platelets by blocking O-glycosylation of these cells. , 1992, Biochemical and biophysical research communications.

[48]  R. Cummings,et al.  The selectin GMP-140 binds to sialylated, fucosylated lactosaminoglycans on both myeloid and nonmyeloid cells , 1991, The Journal of cell biology.

[49]  S. Hakomori,et al.  CD62 and endothelial cell-leukocyte adhesion molecule 1 (ELAM-1) recognize the same carbohydrate ligand, sialyl-Lewis x. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[50]  A. Varki,et al.  GMP-140 binds to a glycoprotein receptor on human neutrophils: evidence for a lectin-like interaction , 1991, The Journal of cell biology.