A DNA biosensor based on resonance light scattering using unmodified gold bipyramids

AbstractWe report on a novel biosensor for determining sequence-specific DNA. It is based on resonance light scattering (RLS) caused by the aggregation of gold bipyramids. These display localized surface plasmon resonance and can be used as a bioprobe. The absorption spectra and the transmission electron micrographs provide visual evidence of the aggregation of the gold bipyramids in the presence of DNA. The RLS intensity of the gold bipyramids increases with the concentration of the target DNA. The method was successfully applied to the determination of a 30-mer single-stranded oligonucleotide and works over the 0.1–10 nM concentration range. FigureThe electrostatic interaction between the ssDNA and gold bipyramids was the driving force to form gold bipyramid-ssDNA complex. After the target DNA added into the gold bipyramid-ssDNA complex suspension, the hybridization between the target DNA and probe ssDNA happened, which caused the aggregation of gold bipyramids.

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