Fructans: beneficial for plants and humans.

The recent cloning of genes encoding fructosyltransferases and fructan exohydrolases has been a major breakthrough in fructan research. Now, fructan metabolism and fructosyltransferase enzymes can be studied at the molecular level. In addition, fructan synthesis and breakdown can be adapted in such a way that tailor-made fructans are produced in plants for use as healthy food ingredients.

[1]  G. Galiba,et al.  Osmotic and salt stresses induced differential alteration in water-soluble carbohydrate content in wheat seedlings , 1998 .

[2]  A. Laere,et al.  Induction of 1-FEH in Mature Chicory Roots Appears to be Related to Low Temperatures Rather than to Leaf Damage , 2002 .

[3]  M. Prud'homme,et al.  Fructans and fructan‐metabolizing enzymes in leaves of Lolium perenne , 2001 .

[4]  S. Clerens,et al.  Defoliation induces fructan 1-exohydrolase II in Witloof chicory roots. Cloning and purification of two isoforms, fructan 1-exohydrolase IIa and fructan 1-exohydrolase IIb. Mass fingerprint of the fructan 1-exohydrolase II enzymes. , 2001, Plant physiology.

[5]  W. Van den Ende,et al.  Drought induces fructan synthesis and 1-SST (sucrose: sucrose fructosyltransferase) in roots and leaves of chicory seedlings (Cichorium intybus L.) , 2000, Planta.

[6]  T. Boller,et al.  Purification, cloning, and functional expression of sucrose:fructan 6-fructosyltransferase, a key enzyme of fructan synthesis in barley. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[7]  G. Noctor,et al.  Stage of development is an important determinant in the effect of nitrate on photoassimilate (13C) partitioning in chicory (Cichorium intybus) , 1997 .

[8]  T. Boller,et al.  Fructan and fructan‐metabolizing enzymes in the growth zone of barley leaves , 1997 .

[9]  D. Hincha,et al.  Plant fructans stabilize phosphatidylcholine liposomes during freeze-drying. , 2000, European journal of biochemistry.

[10]  S. Clerens,et al.  Fructan 1-Exohydrolases. β-(2,1)-Trimmers during Graminan Biosynthesis in Stems of Wheat? Purification, Characterization, Mass Mapping, and Cloning of Two Fructan 1-Exohydrolase Isoforms1,212 , 2003, Plant Physiology.

[11]  P. Verhaert,et al.  Purification and characterization of fructan: fructan fructosyl transferase from chicory (Cichorium intybus L.) roots , 1996, Planta.

[12]  G. Spangenberg,et al.  Isolation and characterisation of a fructosyltransferase gene from perennial ryegrass (Lolium perenne) , 2002 .

[13]  M. Prud'homme,et al.  Rise of fructan exohydrolase activity in stubble of Lolium perenne after defoliation is decreased by uniconazole, an inhibitor of the biosynthesis of gibberellins , 1997 .

[14]  F. Lelièvre,et al.  Production, persistence, and water-soluble carbohydrate accumulation in 21 contrasting populations of Dactylis glomerata L. subjected to severe drought in the south of France , 1997 .

[15]  Christine M. Williams,et al.  The effect of the daily intake of inulin on fasting lipid, insulin and glucose concentrations in middle-aged men and women , 1999, British Journal of Nutrition.

[16]  L. Willmitzer,et al.  Transgenic potato tubers accumulate high levels of 1-kestose and nystose: functional identification of a sucrose sucrose 1-fructosyltransferase of artichoke (Cynara scolymus) blossom discs. , 1997, The Plant journal : for cell and molecular biology.

[17]  Chang-Sheng Wang,et al.  Effects of nitrogen deficiency on accumulation of fructan and fructan metabolizing enzyme activities in sink and source leaves of barley (Hordeum vulgare) , 1996 .

[18]  J. Edelman,et al.  THE MECHANISIM OF FRUCTOSAN METABOLISM IN HIGHER PLANTS AS EXEMPLIFIED IN HELIANTHUS TUBEROSUS , 1968 .

[19]  D. Hincha,et al.  Specific effects of fructo- and gluco-oligosaccharides in the preservation of liposomes during drying. , 2002, Glycobiology.

[20]  U. Simmen,et al.  Sucrose:Fructan 6-Fructosyltransferase, a Key Enzyme for Diverting Carbon from Sucrose to Fructan in Barley Leaves , 1995, Plant physiology.

[21]  B. de Kruijff,et al.  Fructans interact strongly with model membranes. , 1998, Biochimica et biophysica acta.

[22]  C. Pollock,et al.  Carbon allocation and sugar status in individual cells of barley leaves affects expression of sucrose:fructan 6-fructosyltransferase gene , 2001 .

[23]  N. J. Chatterton,et al.  Structure and quantity of fructan oligomers in oat (Avena spp.) , 1993 .

[24]  A. V. Van Dijken,et al.  Fructan of the inulin neoseries is synthesized in transgenic chicory plants (Cichorium intybus L.) harbouring onion (Allium cepa L.) fructan:fructan 6G-fructosyltransferase. , 1997, The Plant journal : for cell and molecular biology.

[25]  D. Livingston.  Nonstructural carbohydrate accumulation in winter oat crowns before and during cold hardening , 1991 .

[26]  A. Koops,et al.  High level fructan accumulation in a transgenic sugar beet , 1998, Nature Biotechnology.

[27]  P. Verhaert,et al.  Cloning and functional analysis of chicory root fructan1-exohydrolase I (1-FEH I): a vacuolar enzyme derivedfrom a cell-wall invertase ancestor? Mass fingerprint of the 1-FEH I enzyme. , 2000, The Plant journal : for cell and molecular biology.

[28]  T. Boller,et al.  Light and sugar regulation of the barley sucrose : fructan 6-fructosyltransferase promoter , 2001 .

[29]  A. Morvan-Bertrand,et al.  Roles of the fructans from leaf sheaths and from the elongating leaf bases in the regrowth following defoliation of Lolium perenne L. , 2001, Planta.

[30]  N. Shiomi.  Purification and characterisation of 6G-fructosyltransferase from the roots of asparagus (Asparagus officinalis L.) , 1981 .

[31]  W. Van den Ende,et al.  The role of fructan in flowering of Campanula rapunculoides. , 2000, Journal of experimental botany.

[32]  N. Delzenne,et al.  Effects of fructans-type prebiotics on lipid metabolism. , 2001, The American journal of clinical nutrition.

[33]  C. Henson,et al.  Purification and Characterization of an Oat Fructan Exohydrolase That Preferentially Hydrolyzes [beta]-2,6-Fructans , 1996, Plant physiology.

[34]  W. Van den Ende,et al.  Sucrose assimilation during early developmental stages of chicory (Cichorium intybus L.) plants , 2001, Planta.

[35]  L. Willmitzer,et al.  Transgenic potato (Solanum tuberosum) tubers synthesize the full spectrum of inulin molecules naturally occurring in globe artichoke (Cynara scolymus) roots. , 2000, Proceedings of the National Academy of Sciences of the United States of America.

[36]  G R Gibson,et al.  Selective stimulation of bifidobacteria in the human colon by oligofructose and inulin. , 1995, Gastroenterology.

[37]  S. Smeekens,et al.  Fructans insert between the headgroups of phospholipids. , 2001, Biochimica et biophysica acta.

[38]  H. Pontis,et al.  Protein kinase and phosphatase activities are involved in fructan synthesis initiation mediated by sugars , 2001, Planta.

[39]  T. Boller,et al.  Inulin synthesis by a combination of purified fructosyltransferases from tubers of Helianthus tuberosus , 1996, FEBS letters.

[40]  D. Obenland,et al.  Fructan Synthesis in Excised Barley Leaves (Identification of Two Sucrose-Sucrose Fructosyltransferases Induced by Light and Their Separation from Constitutive Invertases) , 1993, Plant physiology.

[41]  Chungui Lu,et al.  Rubisco Small Subunit, Chlorophylla/b-Binding Protein and Sucrose:Fructan-6-Fructosyl Transferase Gene Expression and Sugar Status in Single Barley Leaf Cells in Situ. Cell Type Specificity and Induction by Light1 , 2002, Plant Physiology.

[42]  T. Boller,et al.  Fructan synthesis in transgenic tobacco and chicory plants expressing barley sucrose:fructan 6‐fructosyltransferase , 1997, FEBS letters.

[43]  C. Pollock,et al.  Structural analysis of oligomeric fructans from excised leaves of Lolium temulentum , 1992 .

[44]  Noël,et al.  Involvement of sucrose synthase in sucrose synthesis during mobilization of fructans in dormant Jerusalem artichoke tubers. , 2000, Plant science : an international journal of experimental plant biology.

[45]  O. Junttila,et al.  Endogenous gibberellins in Lolium perenne and influence of defoliation on their contents in elongating leaf bases and in leaf sheaths , 2001 .

[46]  Chang-Sheng Wang,et al.  Fructan accumulation induced by nitrogen deficiency in barley leaves correlates with the level of sucrose:fructan 6-fructosyltransferase mRNA , 2000, Planta.

[47]  N. J. Chatterton,et al.  Fructan oligomers in Poa ampla , 1997 .

[48]  M. Frehner,et al.  Localization of Fructan Metabolism in the Vacuoles Isolated from Protoplasts of Jerusalem Artichoke Tubers (Helianthus tuberosus L.). , 1984, Journal of plant physiology.

[49]  L. Willmitzer,et al.  Differences in chain length distribution of inulin from Cynara scolymus and Helianthus tuberosus are reflected in a transient plant expression system using the respective 1‐FFT cDNAs , 1998, FEBS letters.

[50]  P. Hedley,et al.  Exon skipping induced by cold stress in a potato invertase gene transcript. , 1996, Nucleic acids research.

[51]  A. Hourmant,et al.  In vitro bulb development in shallot (Allium cepa L. Aggregatum Group): effects of anti-gibberellins, sucrose and light. , 2002, Annals of botany.

[52]  A. Koops,et al.  Purification and Characterization of the Enzymes of Fructan Biosynthesis in Tubers of Helianthus tuberosus Colombia (II. Purification of Sucrose:Sucrose 1-Fructosyltransferase and Reconstitution of Fructan Synthesis in Vitro with Purified Sucrose:Sucrose 1-Fructosyltransferase and Fructan:Fructan 1- , 1996, Plant physiology.

[53]  G. Gibson,et al.  The effects of the novel bifidogenic trisaccharide, neokestose, on the human colonic microbiota , 2002 .

[54]  N. Shiomi.  Properties of Fructosyltransferases Involved in the Synthesis of Fructan in Liliaceous Plants , 1989 .

[55]  R. L. Bieleski,et al.  Fructan Hydrolysis Drives Petal Expansion in the Ephemeral Daylily Flower , 1993, Plant physiology.

[56]  N. J. Chatterton,et al.  Characterization of fructan biosynthesis in big bluegrass (Poa secunda) , 2002 .

[57]  S. Baumgartner,et al.  Structure of fructans in roots and leaf tissues of Lolium perenne , 2001 .

[58]  P. Weisbeek,et al.  Improved Performance of Transgenic Fructan-Accumulating Tobacco under Drought Stress , 1995, Plant physiology.

[59]  P. John,et al.  Localization of the Enzymes of Fructan Metabolism in Vacuoles Isolated by a Mechanical Method from Tubers of Jerusalem Artichoke (Helianthus tuberosus L.). , 1989, Plant physiology.

[60]  A. Laere,et al.  De-novo synthesis of fructans from sucrose in vitro by a combination of two purified enzymes (sucrose: sucrose 1-fructosyl transferase and fructan: fructan 1-fructosyl transferase) from chicory roots (Cichorium intybus L.) , 1996, Planta.

[61]  T. Boller,et al.  Disaccharide-mediated regulation of sucrose:fructan-6-fructosyltransferase, a key enzyme of fructan synthesis in barley leaves. , 2000, Plant physiology.

[62]  M. Blaut,et al.  Oligofructose and long-chain inulin: influence on the gut microbial ecology of rats associated with a human faecal flora , 2001, British Journal of Nutrition.

[63]  D. Djilianov,et al.  Freezing tolerant tobacco, transformed to accumulate osmoprotectants , 2002 .

[64]  J. Tillberg,et al.  Effects of short-term phosphorus deficiency on carbohydrate storage in sink and source leaves of barley (Hordeum vulgare) , 1997 .

[65]  M. Blaut,et al.  Effects of inulin on faecal bifidobacteria in human subjects , 1999, British Journal of Nutrition.

[66]  J. Schrezenmeir,et al.  Effects of prebiotics on mineral metabolism. , 2001, The American journal of clinical nutrition.