Validation of a Metallomics Analysis of Placenta Tissue by Inductively-Coupled Plasma Mass Spectrometry

Trace elements can play an important role in maternal health and fetal development, and deficiencies in some essential minerals including zinc and copper have been correlated in some individuals to the development of birth defects and adverse health outcomes later in life. The exact etiology of conditions like preeclampsia and the effects of fetal exposure to toxic metals has not been determined, making the assessment of trace element levels crucial to the elucidation of the causes of conditions like preeclampsia. Previous studies analyzing serum and placenta tissue have produced conflicting findings, suggesting the need for a robust, validated sample preparation and analysis method for the determination of trace elements in placenta. In this report, an acid digestion method and analysis by ICP-MS for a broad metallomics/mineralomics panel of trace elements is developed and validated over three experimental days for inter- and intraday precision and accuracy, linear range, matrix impact, and dilution verification. Spike recovery experiments were performed for the essential elements chromium (Cr), iron (Fe), cobalt (Co), nickel (Ni), copper (Cu), and zinc (Zn), and the toxic elements arsenic (As), cadmium (Cd), and lead (Pb) at levels equal to and in excess of native concentrations in control placenta tissue. The validated method will be essential for the development of scientific studies of maternal health and toxic metal exposure effects in childhood.

[1]  M. Korn,et al.  Evaluation of sample preparation (grinding and sieving) of bivalves, coffee and cowpea beans for multi-element analysis , 2008 .

[2]  J. Burguera,et al.  Recent on-line processing procedures for biological samples for determination of trace elements by atomic spectrometric methods , 2009 .

[3]  K. Haram,et al.  Influence of mineral and vitamin supplements on pregnancy outcome. , 2012, European journal of obstetrics, gynecology, and reproductive biology.

[4]  G. Jiang,et al.  Metallomics in environmental and health related research: Current status and perspectives , 2013 .

[5]  Julie Meeks Gardner,et al.  Child development: risk factors for adverse outcomes in developing countries , 2007, The Lancet.

[6]  E. Nieboer,et al.  Maternal nickel exposure and congenital musculoskeletal defects. , 2008, American journal of industrial medicine.

[7]  J. King,et al.  Effect of zinc supplementation on pregnancy and infant outcomes: a systematic review. , 2012, Paediatric and perinatal epidemiology.

[8]  Z. Bhutta,et al.  Preconception care: nutritional risks and interventions , 2014, Reproductive Health.

[9]  J. D. Boer.  Real-time adjustment of ICP-MS elemental equations , 2000 .

[10]  I. Sargent,et al.  Placental stress and pre-eclampsia: a revised view. , 2009, Placenta.

[11]  Joanna Szpunar,et al.  Metallomics: The Concept and Methodology , 2009 .

[12]  Mcgavi Dd.  RESPIRATORY DISEASE IN FLAX WORKERS. , 1965 .

[13]  A Metin Gülmezoglu,et al.  WHO analysis of causes of maternal death: a systematic review , 2006, The Lancet.

[14]  Seemalata Jain,et al.  The Role of Calcium, Magnesium, and Zinc in Pre-Eclampsia , 2010, Biological Trace Element Research.

[15]  M. A. Levine,et al.  Aspects of bioanalytical method validation for the quantitative determination of trace elements. , 2011, Bioanalysis.

[16]  R. P. Thompson,et al.  Are iron-folate supplements harmful? , 1987, The American journal of clinical nutrition.

[17]  A. Sussulini,et al.  Combination of PAGE and LA-ICP-MS as an analytical workflow in metallomics: state of the art, new quantification strategies, advantages and limitations. , 2011, Metallomics : integrated biometal science.

[18]  Kazuya Yoshida,et al.  Relationship between Body Mass Index and Minerals in Male Japanese Adults , 2006 .

[19]  João Paulo Souza,et al.  Risk Factors of Pre-Eclampsia/Eclampsia and Its Adverse Outcomes in Low- and Middle-Income Countries: A WHO Secondary Analysis , 2014, PloS one.

[20]  A. Scheynius,et al.  Anthroposophic lifestyle influences the concentration of metals in placenta and cord blood. , 2015, Environmental research.

[21]  C. Case,et al.  Development of a routine method for the determination of trace metals in whole blood by magnetic sector inductively coupled plasma mass spectrometry with particular relevance to patients with total hip and knee arthroplasty. , 2001, Clinical chemistry.

[22]  N. Sebire,et al.  Optimising sample collection for placental research. , 2014, Placenta.

[23]  T. Hirano,et al.  Zinc homeostasis and signaling in health and diseases , 2011, JBIC Journal of Biological Inorganic Chemistry.

[24]  Mohammad Safiqul Islam,et al.  Comparative Study of Serum Zinc, Copper, Manganese, and Iron in Preeclamptic Pregnant Women , 2013, Biological Trace Element Research.

[25]  D. James,et al.  Zinc supplementation during pregnancy: a double blind randomised controlled trial. , 1989, BMJ.

[26]  E. George.  New approaches for managing preeclampsia: clues from clinical and basic research. , 2014, Clinical therapeutics.

[27]  J. C. Smith,et al.  Plasma-zinc and copper in pregnancy and after oral contraceptives. , 1968, Lancet.

[28]  R. Black,et al.  Micronutrients in pregnancy , 2001, British Journal of Nutrition.

[29]  I. Bygbjerg,et al.  Double Burden of Noncommunicable and Infectious Diseases in Developing Countries , 2012, Science.

[30]  T. Tulchinsky.  Micronutrient Deficiency Conditions: Global Health Issues , 2010 .

[31]  Development of an Analytical Method for Assessment of Silver Nanoparticle Content in Biological Matrices by Inductively Coupled Plasma Mass Spectrometry , 2015, Biological Trace Element Research.

[32]  F. Parvez,et al.  Water Arsenic Exposure and Children’s Intellectual Function in Araihazar, Bangladesh , 2004, Environmental health perspectives.

[33]  Lei Jin,et al.  Placental concentrations of mercury, lead, cadmium, and arsenic and the risk of neural tube defects in a Chinese population. , 2013, Reproductive toxicology.

[34]  B. A. Bassiouni,et al.  Maternal and fetal plasma zinc in pre-eclampsia. , 1979, European journal of obstetrics, gynecology, and reproductive biology.

[35]  K. Murata,et al.  Relationships between trace element concentrations in chorionic tissue of placenta and umbilical cord tissue: potential use as indicators for prenatal exposure. , 2013, Environment international.

[36]  D. Williams,et al.  The Biological Chemistry of the Elements , 1991 .

[37]  Z. Chai,et al.  Metallomics insights for in vivo studies of metal based nanomaterials. , 2013, Metallomics : integrated biometal science.

[38]  Herbert L. Needleman,et al.  Low-Level Environmental Lead Exposure and Children’s Intellectual Function: An International Pooled Analysis , 2005, Environmental health perspectives.

[39]  G. Shaw,et al.  Periconceptional nutrient intakes and risks of orofacial clefts in California , 2013, Pediatric Research.

[40]  Zhenyu Yang,et al.  Iron, Oxidative Stress and Gestational Diabetes , 2014, Nutrients.

[41]  Zhongqi Cheng,et al.  Water Manganese Exposure and Children’s Intellectual Function in Araihazar, Bangladesh , 2004, Environmental health perspectives.

[42]  James M. Harrington,et al.  Analysis of Human Serum and Whole Blood for Mineral Content by ICP-MS and ICP-OES: Development of a Mineralomics Method , 2014, Biological Trace Element Research.

[43]  James M. Roberts,et al.  Hypertension in Pregnancy: Executive Summary , 2013 .