Strain differences in expression of virulence by the 90 kilobase pair virulence plasmid of Salmonella serovar Typhimurium.

A number of plasmidless strains were obtained by curing the 90 kilobase pair (kb) virulence plasmid from six strains, C5, TML, W118, SR11, LT2 and Fisher, of Salmonella serovar Typhimurium. A number of transposon (Tn5) tagged 90 kb plasmids, also derived from these Typhimurium strains, were then transferred back into these plasmidless strains. Plasmid-cured strains, reconstituted strains, and the parental strains were tested for their virulence in BALB/c mice. There were two groups of Typhimurium strains: one required the 90 kb plasmid to express high virulence (LD50 less than 50 bacteria), and the other, regardless of the presence or absence of the 90 kb plasmid, maintained the same level of virulence at LD50 = 10 to 7 x 10(5) bacteria. Among the plasmidless strains, there were strains with a virulence level as low as LD50 = 10(7) bacteria, which was unaffected by the presence of the 90 kb plasmid.

[1]  R. Curtiss,et al.  Cloning and transposon insertion mutagenesis of virulence genes of the 100-kilobase plasmid of Salmonella typhimurium , 1988, Infection and immunity.

[2]  D. Rowley,et al.  Transduction of virulence within the species Salmonella typhimurium. , 1956, Journal of general microbiology.

[3]  V. Mathan,et al.  The colonization of Peyer's patches by a strain of Salmonella typhimurium cured of the cryptic plasmid. , 1986, The Journal of infectious diseases.

[4]  M. Popoff,et al.  Cloning and expression of plasmid DNA sequences involved in Salmonella serotype Typhimurium virulence , 1989, Molecular microbiology.

[5]  R. Curtiss,et al.  Plasmid-associated virulence of Salmonella typhimurium , 1987, Infection and immunity.

[6]  L. Baron,et al.  Novel virulence properties of the Salmonella typhimurium virulence-associated plasmid: immune suppression and stimulation of splenomegaly. , 1989, Plasmid.

[7]  P Berg,et al.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. , 1977, Journal of molecular biology.

[8]  E. Southern.  Detection of specific sequences among DNA fragments separated by gel electrophoresis. , 1975, Journal of molecular biology.

[9]  P. Mäkelä,et al.  Localization by insertion mutagenesis of a virulence-associated region on the Salmonella typhimurium 96 kilobase pair plasmid. , 1989, Microbial pathogenesis.

[10]  P. Reeves,et al.  Mediation of serum resistance in Salmonella typhimurium by an 11-kilodalton polypeptide encoded by the cryptic plasmid. , 1987, The Journal of infectious diseases.

[11]  M. Popoff,et al.  Virulence-associated plasmids of Salmonella serotype Typhimurium in experimental murine infection. , 1986, Annales de l'Institut Pasteur. Microbiologie.

[12]  L. Reed,et al.  A SIMPLE METHOD OF ESTIMATING FIFTY PER CENT ENDPOINTS , 1938 .

[13]  D. Rabert,et al.  Plasmid-associated resistance of Salmonella typhimurium to complement activated by the classical pathway , 1987, Infection and immunity.

[14]  D. Rabert,et al.  Association of adhesive, invasive, and virulent phenotypes of Salmonella typhimurium with autonomous 60-megadalton plasmids , 1982, Infection and immunity.

[15]  L. Baron,et al.  The virulence plasmids of Salmonella serovars typhimurium, choleraesuis, dublin, and enteritidis, and the cryptic plasmids of Salmonella serovars copenhagen and sendai belong to the same incompatibility group, but not those of Salmonella serovars durban, gallinarum, give, infantis and pullorum. , 1990, Microbial pathogenesis.

[16]  M. Rhen,et al.  Molecular organization of genes constituting the virulence determinant on the Salmonella typhimurium 96 kilobase pair plasmid , 1989, FEBS letters.

[17]  S. T. Liu,et al.  Rapid procedure for detection and isolation of large and small plasmids , 1981, Journal of bacteriology.

[18]  K. Miura,et al.  PREPARATION OF TRANSFORMING DEOXYRIBONUCLEIC ACID BY PHENOL TREATMENT. , 1963, Biochimica et biophysica acta.

[19]  P. Gemski,et al.  Non-smooth mutants of Salmonella typhimurium: differentiation by phage sensitivity and genetic mapping. , 1972, Journal of general microbiology.

[20]  C. Turnbough,et al.  Genetic mapping of novel virulence determinants of Salmonella typhimurium to the region between trpD and supD. , 1986, Microbial pathogenesis.

[21]  B A Stocker,et al.  A Salmonella typhimurium virulence gene linked to flg , 1989, Infection and immunity.

[22]  R. Stephan,et al.  Epidemiology of virulence-associated plasmids and outer membrane protein patterns within seven common Salmonella serotypes , 1985 .

[23]  G. Cornelis,et al.  A new method for the physical and genetic mapping of large plasmids: application to the localisation of the virulence determinants on the 90 kb plasmid of Salmonella typhimurium. , 1987, Microbial pathogenesis.