Advances in Brief A Novel 4-cM MinimallyDeletedRegionon ChromosomellplS . l Associatedwith High Grade Nonmucmous Epithelial Ovarian Carcinomas 1

Prior cytogenetic and restriction fragment length polymorphism stud Ins have demonstrated that allelic deletion of chromosome lip is common in humsa Invasive epithellal ovarian tumors. To construct a highly de tailed deletion map of chromosome lip, we used 13 polymorphIc micro satellite CA repeat primers to Identify regions harboring potential tumor suppressor genes, Twenty-three of48 samples (48%) ofinvaslve epithelial ovarian esncer showed LOH involving at least one locus, consistent with prior studies. None of the five mucinous tumors showed allele deletion at any of the 13 prImers, suggesting that loss of heterozygosity at chrome some lip may not be Involved In the pathogenesls of mudnous ovarian cancer. Two separate minimisily deleted regions were identified In non mudnous ovarian cancer. The first Is an il-cM region on chromosome iipiS.S-.iS.3 that extends from D11S2071 to D11S988 and Includes the HRAS locus. The second Is a novel 4-cM region on iipi5.i, defined by marker D1JSJ31O. Deletion of both regions at iipi5.5—iS.3 and iipi5.i Is strongly associated with high grade nonmucinous epithelial ovarian cancer.

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