Acquisition of triploid germplasms by controlled hybridisation between diploid and tetraploid in Chinese jujube

ABSTRACT‘Chenguang’ is the first released tetraploid cultivar (2n = 4x = 48) of Chinese jujube (Ziziphus jujuba Mill.) induced by colchicine. The purpose of this study was to create triploid germplasms by using ‘Chenguang’ as a parent. The controlled crosses between autotetraploid ‘Chenguang’ and diploid ‘Dongzao’ were carried out from 2013 to 2015 by maintaining the 2 parents in a nylon net and employing bees as pollinator. A total of 606 fruits were produced, yet only 17 seeds were obtained from ‘Dongzao’ and none from ‘Chenguang’. Direct sowing of the seeds in greenhouse showed a higher seedling rate than embryo culture. Four progeny plants were eventually obtained in the greenhouse and all of them were confirmed as authentic hybrids by SSR marker technique. At last, 3 triploids and 1 diploid were identified by flow cytometric analysis. Consequently, tetraploid ‘Chenguang’ with high pollen vitality and pollen number per anther could be used as a reliable 2n pollen provider, and the 2x × 4x cross combin...

[1]  P. Arens,et al.  Induction of viable 2n pollen in sterile Oriental × Trumpet Lilium hybrids , 2016 .

[2]  A. Sato,et al.  Advances in table grape breeding in Japan , 2016, Breeding science.

[3]  Sun Hongyan,et al.  Diversity of phenotypic variation of polyploid plants new germplasm in pear (Pyrus communis L.) , 2016 .

[4]  R. Ren,et al.  In vitro embryo rescue culture of F1 progenies from crosses between different ploidy grapes. , 2015, Genetics and molecular research : GMR.

[5]  Yang Liu,et al.  Nonsolvable D2-groups , 2015 .

[6]  Ping Liu,et al.  Hybridization using a new male-sterile germplasm as the female parent in Chinese jujube. , 2015 .

[7]  Wang Xiaoqing,et al.  Morphological, cytological and fertility consequences of a spontaneous tetraploid of the diploid pear (Pyrus pyrifolia Nakai) cultivar 'Cuiguan'. , 2015 .

[8]  Ping Liu,et al.  Genome-Wide Characterization of Simple Sequence Repeat (SSR) Loci in Chinese Jujube and Jujube SSR Primer Transferability , 2015, PloS one.

[9]  Zhi-Guo Liu,et al.  The complex jujube genome provides insights into fruit tree biology , 2014, Nature Communications.

[10]  S. A. Melnik,et al.  Breeding assessment of heteroploid crosses in the development of triploid apple varieties , 2014, Russian Journal of Genetics: Applied Research.

[11]  E. Sedov.  Apple breeding programs and methods, their development and improvement , 2014, Russian Journal of Genetics: Applied Research.

[12]  Ren Hai-ya.  Advances in research on embryo culture of Chinese Jujube , 2014 .

[13]  Y. Hoshino,et al.  Interploid and intraploid hybridizations to produce polyploid Haskap (Lonicera caerulea var. emphyllocalyx) plants , 2014, Euphytica.

[14]  M. Liu,et al.  ADVANCES OF RESEARCH ON GERMPLASM RESOURCES OF CHINESE JUJUBE , 2013 .

[15]  Li Xue-sheng.  Discovery and Identification of Natural Triploid Ploidy of Chinese Jujube Cultivar‘Pingguozao’ , 2013 .

[16]  Qiang Xu,et al.  Production and molecular characterization of diploid and tetraploid somatic cybrid plants between male sterile Satsuma mandarin and seedy sweet orange cultivars , 2013, Plant Cell, Tissue and Organ Culture (PCTOC).

[17]  Zhao Zhihui,et al.  ‘Chenguang’, a new tetraploid Chinese jujube cultivar , 2012 .

[18]  P. Ollitrault,et al.  Extensive citrus triploid hybrid production by 2x × 4x sexual hybridizations and parent-effect on the length of the juvenile phase , 2012, Plant Cell Reports.

[19]  L. Ping.  Advances in Ploidy Breeding of Fruit Trees , 2012 .

[20]  Wu Yong-jie.  Germplas Development of Triploid Grapevine and Rapid Identification of Chromosomes Ploidy , 2011 .

[21]  J. Grosser,et al.  Protoplast fusion for production of tetraploids and triploids: applications for scion and rootstock breeding in citrus , 2011, Plant Cell, Tissue and Organ Culture (PCTOC).

[22]  Mengjun Liu.  Chinese Jujube: Botany and Horticulture , 2010 .

[23]  Aroldo Cisneros,et al.  Embryo rescue and plant regeneration following interspecific crosses in the genus Hylocereus (Cactaceae) , 2010, Euphytica.

[24]  L. Dengke,et al.  The influencing factors of the fruiting characteristics of Ziziphus jujuba. , 2010 .

[25]  Huang Wei,et al.  Establishment of suspension cell cultures and cell growth characteristics of Xanthoceras sorbifolia. , 2010 .

[26]  M. Liu,et al.  Plant regeneration from immature embryo in Chinese jujube (Ziziphus jujuba Mill.). , 2009 .

[27]  Z. Guo-jun.  Embryo Rescue and Identification of Hybrids between Diploid Grape and Tetraploid Grape , 2008 .

[28]  M. Tan,et al.  Production of two mandarin × trifoliate orange hybrid populations via embryo rescue with verification by SSR analysis , 2007, Euphytica.

[29]  M. Mii,et al.  Variation of ploidy level in inter-section hybrids obtained by reciprocal crosses between tetraploid Primula denticulata (2n = 4x = 44) and diploid P. rosea (2n = 2x = 22) , 2007 .

[30]  S. Oka,et al.  Somatic hybridization by electrofusion of banana protoplasts , 2002, Euphytica.

[31]  H. Yi,et al.  Somatic hybrids between navel orange (Citrus sinensis) and grapefruit (C. paradisi) for seedless triploid breeding , 2000, Euphytica.