Comparative evaluation of nanofibrous scaffolding for bone regeneration in critical-size calvarial defects.

In a previous study we found that nanofibrous poly(l-lactic acid) (PLLA) scaffolds mimicking collagen fibers in size were superior to solid-walled scaffolds in promoting osteoblast differentiation and bone formation in vitro. In this study we used an in vivo model to confirm the biological properties of nanofibrous PLLA scaffolds and to evaluate how effectively they support bone regeneration against solid-walled scaffolds. The scaffolds were implanted in critical-size defects made on rat calvarial bones. Compared with solid-walled scaffolds, nanofibrous scaffolds supported substantially more new bone tissue formation, which was confirmed by micro-computed tomography measurement and von Kossa staining. Goldner's trichrome staining showed abundant collagen deposition in nanofibrous scaffolds but not in the control solid-walled scaffolds. The cells in these scaffolds were immuno-stained strongly for Runx2 and bone sialoprotein (BSP). In contrast, solid-walled scaffolds implanted in the defects were stained weakly with trichrome, Runx2, and BSP. These in vivo results demonstrate that nanofibrous architecture enhances osteoblast differentiation and bone formation.

[1]  P. Ma,et al.  Cell and biomolecule delivery for regenerative medicine , 2010, Science and technology of advanced materials.

[2]  Peter X Ma,et al.  Induction of osteoblast differentiation phenotype on poly(L-lactic acid) nanofibrous matrix. , 2008, Biomaterials.

[3]  E. Loboa,et al.  Effect of varied ionic calcium on human adipose-derived stem cell mineralization. , 2010, Tissue engineering. Part A.

[4]  Peter X Ma,et al.  Nano-fibrous scaffolding promotes osteoblast differentiation and biomineralization. , 2007, Biomaterials.

[5]  Peter X Ma,et al.  Nano-fibrous poly(L-lactic acid) scaffolds with interconnected spherical macropores. , 2004, Biomaterials.

[6]  Andrés J. García,et al.  Mineralization capacity of Runx2/Cbfa1-genetically engineered fibroblasts is scaffold dependent. , 2006, Biomaterials.

[7]  G. Stein,et al.  The influence of type I collagen on the development and maintenance of the osteoblast phenotype in primary and passaged rat calvarial osteoblasts: modification of expression of genes supporting cell growth, adhesion, and extracellular matrix mineralization. , 1995, Experimental cell research.

[8]  P. Ma,et al.  Synthetic nano-scale fibrous extracellular matrix. , 1999, Journal of biomedical materials research.

[9]  William V Giannobile,et al.  The enhancement of osteogenesis by nano-fibrous scaffolds incorporating rhBMP-7 nanospheres. , 2007, Biomaterials.

[10]  Peter X Ma,et al.  Biomimetic materials for tissue engineering. , 2008, Advanced drug delivery reviews.

[11]  B. Pourdeyhimi,et al.  In situ collagen polymerization of layered cell-seeded electrospun scaffolds for bone tissue engineering applications. , 2010, Tissue engineering. Part C, Methods.

[12]  Peter X Ma,et al.  Macroporous and nanofibrous polymer scaffolds and polymer/bone-like apatite composite scaffolds generated by sugar spheres. , 2006, Journal of biomedical materials research. Part A.

[13]  Shuguang Zhang,et al.  Self-organization of a chiral D-EAK16 designer peptide into a 3D nanofiber scaffold. , 2008, Macromolecular bioscience.

[14]  Peter X Ma,et al.  Nano-fibrous scaffolding architecture selectively enhances protein adsorption contributing to cell attachment. , 2003, Journal of biomedical materials research. Part A.

[15]  Gary E. Wnek,et al.  Electrospinning of Nanofiber Fibrinogen Structures , 2003 .

[16]  Samuel I Stupp,et al.  Self-assembling peptide amphiphile nanofiber matrices for cell entrapment. , 2005, Acta biomaterialia.

[17]  Peter X Ma,et al.  Bone regeneration on computer-designed nano-fibrous scaffolds. , 2006, Biomaterials.

[18]  J. Vacanti,et al.  A biodegradable nanofiber scaffold by electrospinning and its potential for bone tissue engineering. , 2003, Biomaterials.

[19]  J O Hollinger,et al.  The critical size defect as an experimental model to test bone repair materials. , 1990, The Journal of craniofacial surgery.

[20]  H. Matloub,et al.  Osteogenesis in calvarial defects: contribution of the dura, the pericranium, and the surrounding bone in adult versus infant animals. , 2003, Plastic and reconstructive surgery.

[21]  P. Ma,et al.  Biodegradable polymer scaffolds with well-defined interconnected spherical pore network. , 2001, Tissue engineering.

[22]  P. Ma,et al.  Synthetic nano-fibrillar extracellular matrices with predesigned macroporous architectures. , 2000, Journal of biomedical materials research.

[23]  Cato T Laurencin,et al.  Electrospun nanofibrous structure: a novel scaffold for tissue engineering. , 2002, Journal of biomedical materials research.

[24]  Laura A. Smith,et al.  Nano-fibrous scaffolds for tissue engineering. , 2004, Colloids and surfaces. B, Biointerfaces.

[25]  Peter X Ma,et al.  The effect of surface area on the degradation rate of nano-fibrous poly(L-lactic acid) foams. , 2006, Biomaterials.