Determination of sub-nanomole amounts of hydrogen peroxide using an immobilized enzyme flow cell. Application to the determination of ethanol

Abstract A flow-injection system was developed to measure hydrogen peroxide using the luminol-4-idodophenol reaction and an immobilized horseradish peroxidase flow cell. The immobilization procedure adopted allowed 17.06 mg of peroxidase to be attached covalently to 1 g of controlled-pore glass. Hydrogen peroxide could be quantitatively detected down to a limiting concentration of 1 × 10 −8 M, which corresponded to 1 pmol. The incorporation of immobilized alcohol oxidase into the flow system allowed the determination of ethanol down to the same low levels.

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