In situ liquid-cell electron microscopy of colloid aggregation and growth dynamics.

We report on real-time observations of the aggregation of gold nanoparticles using a custom-made liquid cell that allows for in situ electron microscopy. Process kinetics and fractal dimension of the aggregates are consistent with three-dimensional cluster-cluster diffusion-limited aggregation, even for large aggregates, for which confinement effects are expected. This apparent paradox was resolved through in situ observations of the interactions between individual particles as well as clusters at various stages of the aggregation process that yielded the large aggregates. The liquid cell described herein facilitates real-time observations of various processes in liquid media with the high resolution of the electron microscope.

[1]  D. Peckys,et al.  Nanoscale Imaging of Whole Cells Using a Liquid Enclosure and a Scanning Transmission Electron Microscope , 2009, PloS one.

[2]  Huang,et al.  Limits of the fractal dimension for irreversible kinetic aggregation of gold colloids. , 1985, Physical review letters.

[3]  F. Huang,et al.  Progress of nanocrystalline growth kinetics based on oriented attachment. , 2010, Nanoscale.

[4]  D. Weitz,et al.  Fractal structures formed by kinetic aggregation of aqueous gold colloids , 1984 .

[5]  Timothy O. Drews,et al.  A mathematical model for crystal growth by aggregation of precursor metastable nanoparticles. , 2005, The journal of physical chemistry. B.

[6]  Meakin,et al.  Kinetics of coagulation with fragmentation: Scaling behavior and fluctuations. , 1986, Physical review letters.

[7]  A. Alivisatos,et al.  Observation of Single Colloidal Platinum Nanocrystal Growth Trajectories , 2009, Science.

[8]  宁北芳,et al.  疟原虫var基因转换速率变化导致抗原变异[英]／Paul H, Robert P, Christodoulou Z, et al//Proc Natl Acad Sci U S A , 2005 .

[9]  Banfield,et al.  Imperfect oriented attachment: dislocation generation in defect-free nanocrystals , 1998, Science.

[10]  D. Peckys,et al.  Electron microscopy of whole cells in liquid with nanometer resolution , 2009, Proceedings of the National Academy of Sciences.

[11]  J. F. Creemer,et al.  A MEMS Reactor for Atomic-Scale Microscopy of Nanomaterials Under Industrially Relevant Conditions , 2010, Journal of Microelectromechanical Systems.

[12]  J. F. Creemer,et al.  Atomic-scale electron microscopy at ambient pressure. , 2008, Ultramicroscopy.

[13]  Hwei-Ling Peng,et al.  Novel microchip for in situ TEM imaging of living organisms and bio-reactions in aqueous conditions. , 2008, Lab on a chip.

[14]  N. de Jonge,et al.  Microfluidic System for Transmission Electron Microscopy , 2010, Microscopy and Microanalysis.

[15]  H. Bau,et al.  The Nanoaquarium: A Platform for In Situ Transmission Electron Microscopy in Liquid Media , 2010, Journal of Microelectromechanical Systems.

[16]  P. Krapivsky,et al.  Nonscaling and source-induced scaling behaviour in aggregation model of movable monomers and immovable clusters , 1991 .

[17]  J. F. Creemer,et al.  Wafer-level assembly and sealing of a MEMS nanoreactor for in situ microscopy , 2010 .

[18]  E. Longo,et al.  A kinetic model to describe nanocrystal growth by the oriented attachment mechanism. , 2005, Chemphyschem : a European journal of chemical physics and physical chemistry.

[19]  Park,et al.  Mechanism of Formation of Monodispersed Colloids by Aggregation of Nanosize Precursors. , 1999, Journal of colloid and interface science.

[20]  P. Meakin,et al.  Universal diffusion-limited colloid aggregation , 1990 .

[21]  F. Ross,et al.  Dynamic microscopy of nanoscale cluster growth at the solid–liquid interface , 2003, Nature materials.

[22]  Peter C Searson,et al.  Quantifying electrochemical nucleation and growth of nanoscale clusters using real-time kinetic data. , 2006, Nano letters.

[23]  R. Rosenfeld.  Nature , 2009, Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery.

[24]  Banfield,et al.  Oriented attachment and growth, twinning, polytypism, and formation of metastable phases: Insights from nanocrystalline TiO2 , 1998 .

[25]  E. Longo,et al.  Tailoring of heterostructures in a SnO2∕TiO2 system by the oriented attachment mechanism , 2007 .

[26]  Model of Formation of Monodispersed Colloids , 2001, cond-mat/0102079.

[27]  G. G. Stokes.  "J." , 1890, The New Yale Book of Quotations.

[28]  R. C. Ball,et al.  Universality in colloid aggregation , 1989, Nature.

[29]  J. Cheon,et al.  Surfactant-assisted elimination of a high energy facet as a means of controlling the shapes of TiO2 nanocrystals. , 2003, Journal of the American Chemical Society.

[30]  Feng Huang,et al.  Two-Stage Crystal-Growth Kinetics Observed during Hydrothermal Coarsening of Nanocrystalline ZnS , 2003 .

[31]  A. Alivisatos,et al.  Nanocrystal diffusion in a liquid thin film observed by in situ transmission electron microscopy. , 2009, Nano letters.

[32]  Andrew G. Glen,et al.  APPL , 2001 .

[33]  Kyung-Sang Cho,et al.  Designing PbSe nanowires and nanorings through oriented attachment of nanoparticles. , 2005, Journal of the American Chemical Society.

[34]  R. L. Penn,et al.  Kinetics of Oriented Aggregation , 2004 .