Dielectric spectroscopy of mammalian cells

Low-frequency dielectric spectroscopy has been usedin situ, i.e. while the cells are still attached to their microsupport, to monitor the changes of biomass accompanying the growth of anchorage-dependent cells. This method, when compared to Aperture Impedance Pulse Spectroscopy (also called electronic sizing), is characterized by a somewhat lower degree of resolution. Suggestions are made on how to determine the capacitance of the spent growth medium alone, still keeping the probe inserted in the bioreactor. This will make dielectric spectroscopy the first trulyin situ, on-line, in real time, non-invasive measure of the biomass.

[1]  G. H. Markx,et al.  Substitution and spreadsheet methods for analysing dielectric spectra of biological systems , 1990, European Biophysics Journal.

[2]  D. Kell,et al.  The dielectric permittivity at radio frequencies and the bruggeman probe: novel techniques for the on-line determination of biomass concentrations in plant cell cultures , 1991 .

[3]  D. Kell,et al.  Dielectric Spectroscopy: a Rapid Method for the Determination of Solvent Biocompatibility During Biotransformations , 1989 .

[4]  A. O. Miller.  Study RNA extracted from HeLa cell polysomes. Isopycnic centrifugation of cytoplasmic particles extracted from normal, poliovirus-infected HeLa cells and mouse A9 cells. , 1972, Archives of biochemistry and biophysics.

[5]  A. Aguilera,et al.  Laser Flow Cytofluorometric Analysis of Htc Cells Synchronized With Hydroxyurea, Nocodazole and Aphidicolin , 1981, Cell and tissue kinetics.

[6]  D. Kell,et al.  Evidence from its temperature dependence that the β-dielectric dispersion of cell suspensions is not due solely to the charging of a static membrane capacitance , 1990, European Biophysics Journal.

[7]  A Irimajiri,et al.  Passive electrical properties of the membrane and cytoplasm of cultured rat basophil leukemia cells. II. Effects of osmotic perturbation. , 1987, Biochimica et biophysica acta.

[8]  Christopher L. Davey,et al.  On the audio- and radio-frequency dielectric behaviour of anchorage-independent, mouse L929-derived LS fibroblasts , 1988 .

[9]  R Fehrenbach,et al.  On-line biomass monitoring by capacitance measurement. , 1992, Journal of biotechnology.

[10]  A. Irimajiri,et al.  Passive electrical properties of the membrane and cytoplasm of cultured rat basophil leukemia cells. I. Dielectric behavior of cell suspensions in 0.01-500 MHz and its simulation with a single-shell model. , 1987, Biochimica et biophysica acta.

[11]  Douglas B. Kell,et al.  Dielectric permittivity of microbial suspensions at radio frequencies: a novel method for the real-time estimation of microbial biomass , 1987 .

[12]  F. Menozzi,et al.  Microbeads and anchorage-dependent eukaryotic cells: the beginning of a new era in biotechnology. , 1989, Advances in biochemical engineering/biotechnology.

[13]  L. Fabry,et al.  Dielectric spectroscopy of mammalian cells , 1993, Cytotechnology.

[14]  Christopher L. Davey,et al.  Real-time monitoring of cellular biomass: Methods and applications , 1990 .