Determination of Dehydroepiandrosterone in Dietary Supplements and Pharmaceutical Products by a Competitive Chemiluminescent Enzyme Immunoassay

Abstract Dehydroepiandrosterone (DHEA), an endogenous steroid hormone, is available in dietary supplements and pharmaceutical products. However, the contents of DHEA in many products are inconsistent with label claims. Excess consumption of DHEA may cause extensive adverse reactions. This study aimed to establish a rapid immunoassay for the determination of DHEA in dietary supplements and pharmaceutical products. An indirect competitive chemiluminescent enzyme immunoassay (ic-CLEIA) was developed based on the generated monoclonal antibody. The limit of detection for DHEA was 0.21 ng/mL with a linear working range from 0.25 to 10 ng/mL with a R2 value equal to 0.989. The recoveries of the spiked samples as tablets, capsules, and lyophilized powders were from 84.0% to 102.4% (intra-assay) and 89.5% to 105.5% (inter-assay). The results were validated by high-performance liquid chromatography (HPLC) with a high correlation coefficient of 0.937, demonstrating that the developed ic-CLEIA was sensitive for the determination of DHEA in dietary supplements and pharmaceutical products.

[1]  Hongtao Lei,et al.  Rapid detection of adulteration of dehydroepiandrosterone in slimming products by competitive indirect enzyme-linked immunosorbent assay and lateral flow immunochromatography , 2019, Food and Agricultural Immunology.

[2]  Stanislava Ivanova,et al.  HPLC DETECTION OF DEHYDROEPIANDROSTERONE IN FOOD ADDITIVES BY USING NORMAL!PHASE HPLC , 2016 .

[3]  Longhua Xu,et al.  Competitive immunoassay for analysis of bisphenol A in children’s sera using a specific antibody , 2016, Environmental Science and Pollution Research.

[4]  A. Kuryliszyn-Moskal,et al.  Dehydroepiandrosterone (DHEA): Hypes and Hopes , 2014, Drugs.

[5]  R. López-Marure,et al.  Effects of dehydroepiandrosterone on proliferation, migration, and death of breast cancer cells. , 2011, European journal of pharmacology.

[6]  A. Bradley,et al.  Plasma DHEA levels in wild, territorial red squirrels: seasonal variation and effect of ACTH. , 2008, General and comparative endocrinology.

[7]  P. Zou,et al.  Detection of dehydroepiandrosterone and androsterone in a traditional Chinese herbal product , 2007, Food additives and contaminants.

[8]  M. Hill,et al.  A novel radioimmunoassay of 7-oxo-DHEA and its physiological levels , 2007, Steroids.

[9]  T. Higashi,et al.  Gas chromatography and high-performance liquid chromatography of natural steroids. , 2001, Journal of chromatography. A.

[10]  M. Carlson,et al.  Liquid chromatographic determination of dehydroepiandrosterone (DHEA) in dietary supplement products. , 2000, Journal of AOAC International.

[11]  M. Hill,et al.  Immunoassay of 7-hydroxysteroids: 2. Radioimmunoassay of 7α-hydroxy-dehydroepiandrosterone , 1999, The Journal of Steroid Biochemistry and Molecular Biology.

[12]  M. Hill,et al.  Immunoassay of 7-hydroxysteroids: 1. Radioimmunoassay of 7β-hydroxy dehydroepiandrosterone , 1998, The Journal of Steroid Biochemistry and Molecular Biology.

[13]  J. Parasrampuria,et al.  Quality control of dehydroepiandrosterone dietary supplement products. , 1998, JAMA.

[14]  John G Lewis,et al.  Production and characterization of monoclonal antibodies to dehydroepiandrosterone sulfate: Application to direct enzyme-linked immunosorbent assays of dehydroepiandrosterone sulfate and androsterone/epiandrosterone sulfates in plasma , 1996, Steroids.

[15]  E. Baulieu,et al.  Characterization and measurement of dehydroepiandrosterone sulfate in rat brain. , 1981, Proceedings of the National Academy of Sciences of the United States of America.

[16]  Chi Yang,et al.  Preparation and identification of monoclonal antibody against Citreoviridin and development of detection by Ic-ELISA. , 2014, Toxicon : official journal of the International Society on Toxinology.

[17]  M. Dreux,et al.  Some applications of a commercial light scattering detector for liquid chromatography , 1985 .