1,3-Propanediol production from glycerol with Lactobacillus diolivorans.

The aim of this study was to evaluate the natural producer Lactobacillus diolivorans as potential production organism of 1,3-propanediol from glycerol. Different cultivation parameters, such as oxygen supply, feeding-strategy, or medium composition have been tested in batch and fed-batch cultivations. The 1,3-propanediol concentration obtained in batch cultivations was 41.7 g/l. This could be increased to 73.7 g/l in a fed-batch co-feeding glucose and glycerol with a molar ratio of 0.1. Yeast extract as part of the MRS cultivation medium could be replaced by nicotinic acid and riboflavin. Furthermore, the addition of Vitamin B(12) to the culture medium increased production by 15% to a final titer of 84.5 g/l 1,3-propanediol.

[1]  Philippe Soucaille,et al.  Metabolic engineering of Clostridium acetobutylicum for the industrial production of 1,3-propanediol from glycerol. , 2005, Metabolic engineering.

[2]  P. Loubière,et al.  Transient self‐inhibition of the growth of Lactobacillus delbrueckii subsp. bulgaricus in a pH‐regulated fermentor , 2003, Biotechnology and bioengineering.

[3]  Z. Cao,et al.  Characterization of glycerol dehydratase expressed by fusing its α- and β-subunits , 2009, Biotechnology Letters.

[4]  Klaus-Dieter Vorlop,et al.  High-level production of 1,3-propanediol from crude glycerol by Clostridium butyricum AKR102a , 2011, Applied Microbiology and Biotechnology.

[5]  V. Rangaswamy,et al.  1,3-Propanediol production from crude glycerol from Jatropha biodiesel process. , 2011, New biotechnology.

[6]  John A Posada,et al.  Design and analysis of biorefineries based on raw glycerol: addressing the glycerol problem. , 2012, Bioresource technology.

[7]  L. Axelsson,et al.  Utilization of Glycerol as a Hydrogen Acceptor by Lactobacillus reuteri: Purification of 1,3-Propanediol:NAD+ Oxidoreductase , 1990, Applied and environmental microbiology.

[8]  M. A. Foster,et al.  Sugar-glycerol cofermentations in lactobacilli: the fate of lactate , 1992, Journal of bacteriology.

[9]  M. Rogosa,et al.  A MEDIUM FOR THE CULTIVATION OF LACTOBACILLI , 1960 .

[10]  S. Vollenweider,et al.  Production of 3-hydroxypropionaldehyde using a two-step process with Lactobacillus reuteri , 2005, Applied Microbiology and Biotechnology.

[11]  P. Xu,et al.  Microbial fed-batch production of 1,3-propanediol by Klebsiella pneumoniae under micro-aerobic conditions , 2003, Applied Microbiology and Biotechnology.

[12]  C. Nakamura,et al.  Metabolic engineering for the microbial production of 1,3-propanediol. , 2003, Current opinion in biotechnology.

[13]  S. Vollenweider,et al.  3-Hydroxypropionaldehyde: applications and perspectives of biotechnological production , 2004, Applied Microbiology and Biotechnology.

[14]  Yan Sun,et al.  Pilot-scale production of 1,3-propanediol using Klebsiella pneumoniae , 2007 .

[15]  Duane T. Johnson,et al.  The glycerin glut: Options for the value‐added conversion of crude glycerol resulting from biodiesel production , 2007 .

[16]  M. Sauer,et al.  Microbial production of 1,3-propanediol. , 2008, Recent patents on biotechnology.

[17]  Wu Bin.  Fermentative production of 1,3-propanediol from glycerol by Clostridium butyricum , 2004 .

[18]  P. Westermann,et al.  Fermentation of crude glycerol from biodiesel production by Clostridium pasteurianum , 2012, Journal of Industrial Microbiology & Biotechnology.

[19]  J. Zigová,et al.  Advances in biotechnological production of butyric acid , 2000, Journal of Industrial Microbiology and Biotechnology.

[20]  I. Berregi,et al.  Glycerol metabolism and bitterness producing lactic acid bacteria in cidermaking. , 2008, International journal of food microbiology.

[21]  W. Dobrogosz,et al.  Purification and Characterization of Glycerol Dehydratase from Lactobacillus reuteri , 1990, Applied and environmental microbiology.

[22]  J. Swings,et al.  Lactobacillus diolivorans sp. nov., a 1,2-propanediol-degrading bacterium isolated from aerobically stable maize silage. , 2002, International journal of systematic and evolutionary microbiology.

[23]  Masahiro Tanaka,et al.  Novel anaerobic digestion induced by bacterial components for value-added byproducts from high-loading glycerol. , 2012, Bioresource technology.

[24]  Seonghun Kim,et al.  Optimization of Culture Conditions for 1,3-Propanediol Production from Glycerol Using a Mutant Strain of Klebsiella pneumoniae , 2011, Applied Biochemistry and Biotechnology.

[25]  P. T. Vasudevan,et al.  Biodiesel production—current state of the art and challenges , 2008, Journal of Industrial Microbiology & Biotechnology.

[26]  K. Shimizu,et al.  Simultaneous production of 3-hydroxypropionic acid and 1,3-propanediol from glycerol by a recombinant strain of Klebsiella pneumoniae. , 2012, Bioresource technology.

[27]  A. Freund.  Über die Bildung und Darstellung von Trimethylenalkohol aus Glycerin , 1881 .

[28]  Subhash Chand,et al.  Advances in biotechnological production of 1,3-propanediol , 2012 .