Diffusion flame-derived fine particulate matters doped with iron caused genotoxicity in B6C3F1 mice

Potential genotoxic effects of diffusion flame-derived particulate matters (PMs), known to cause various adverse health problems, doped with iron, one of the representative heavy metals frequently found in the atmosphere, were examined. B6C3F1 mice were exposed to PMs [chamber 1 (low), 100; chamber 2 (middle), 200; and chamber 3 (high), 400 mg/m3] for 6 h/day, 5 days/week for one, two and four weeks in 1.5 m3 whole-body inhalation chambers. Our diffusion flame system produced 94.8 and 5.2% fine PM2.5 and PM10, respectively, with 89% of PM2.5 sized between 0.1 and 0.2 mm. Two cytogenetic endpoints were investigated through chromosomal aberration and supravital micronucleus (SMN) assays. Frequencies of cells with chromosome aberration (%) were observed in time- and concentration-dependent manners except in one-week exposure group, as also observed in SMN study. Generally, noniron flame induced less chromosome aberration than iron-doped flame, an indication that iron particles could potentiate urban PM toxicity. The above results indicate our diffusion flame system generated genotoxic fine PMs, whose effects were potentiated by organometallic particles such as iron. Our system can provide reliable PM models for studying the toxicity of urban fine PMs applicable for risk assessment.

[1]  Roel P F Schins,et al.  Inhaled particles and lung cancer. Part A: Mechanisms , 2004, International journal of cancer.

[2]  C. Coopersmith,et al.  Epithelial cells , 1991 .

[3]  M. Hannigan,et al.  Trends in Fine Particle Concentration and Chemical Composition in Southern California , 2000, Journal of the Air & Waste Management Association.

[4]  Jun-Sung Kim,et al.  Comparative study of PM2.5 - and PM10 - induced oxidative stress in rat lung epithelial cells. , 2004, Journal of veterinary science.

[5]  C. Marie,et al.  Ischemia-induced brain iron delocalization: effect of iron chelators. , 1994, Free radical biology & medicine.

[6]  D. Templeton,et al.  Modulation of stellate cell proliferation and gene expression by rat hepatocytes: effect of toxic iron overload. , 2003, Toxicology letters.

[7]  A. Poma,et al.  Monitoring urban air particulate matter (fractions PM 2.5 and PM 10) genotoxicity by plant systems and human cells in vitro: a comparative analysis. , 2002, Teratogenesis, carcinogenesis, and mutagenesis.

[8]  P. S. Nielsen,et al.  Biomarkers for exposure to ambient air pollution--comparison of carcinogen-DNA adduct levels with other exposure markers and markers for oxidative stress. , 1999, Environmental health perspectives.

[9]  A. Baulig,et al.  Biological effects of atmospheric particles on human bronchial epithelial cells. Comparison with diesel exhaust particles. , 2003, Toxicology in vitro : an international journal published in association with BIBRA.

[10]  Steffen Loft,et al.  Biomarkers for exposure to ambient air pollution--comparison of carcinogen-DNA adduct levels with other exposure markers and markers for oxidative stress. , 1999 .

[11]  J. Landolph,et al.  Genotoxicity of samples of nickel refinery dust. , 2003, Toxicological sciences : an official journal of the Society of Toxicology.

[12]  K. Kjaerheim,et al.  Occupational cancer research in the Nordic countries. , 1999, Environmental health perspectives.

[13]  J S Lighty,et al.  Combustion Aerosols: Factors Governing Their Size and Composition and Implications to Human Health , 2000, Journal of the Air & Waste Management Association.

[14]  D. Zmirou,et al.  Urinary 1-hydroxypyrene as a biomarker of polycyclic aromatic hydrocarbons exposure of workers on a contaminated site: influence of exposure conditions. , 2000, Journal of occupational and environmental medicine.

[15]  U. Gelatti,et al.  Monitoring airborne genotoxicants in the rubber industry using genotoxicity tests and chemical analyses. , 2001, Mutation research.

[16]  J. Farber,et al.  Cellular pool of transient ferric iron, chelatable by deferoxamine and distinct from ferritin, that is involved in oxidative cell injury. , 1992, Molecular pharmacology.

[17]  T. Sofuni,et al.  The micronucleus assay using peripheral blood reticulocytes from mitomycin C- and cyclophosphamide-treated rats. , 1992, Mutation research.

[18]  B. S. Kim,et al.  Statistical analysis of in vivo rodent micronucleus assay. , 2000, Mutation research.