论文信息 - ATTOLITER LIQUID CHROMATOGRAPHY USING EXTENDED-NANO CHANNEL FOR SEPARATION OF PROTEINS IN A SINGLE CELL

ATTOLITER LIQUID CHROMATOGRAPHY USING EXTENDED-NANO CHANNEL FOR SEPARATION OF PROTEINS IN A SINGLE CELL

A liquid chromatography using 100-nm scale (extended-nano) channels was developed for single cell analysis. Our method, extended-nano chromatography makes possible an ultrasmall sample (100 aL-1 fL) injection and high efficient separation (~10 5 -10 6 plates/m) using nanofluidic channel as separation column. In particular, hydrophilic interaction (HILIC) and reversed-phase chromatography was developed for separation of biomolecules including proteins in a single cell. In addition, an influence of a specific liquid property in extended-nano space was investigated for higher separation efficiency.

K. Mawatari | T. Kitamori | H. Shimizu | Ryo Ishibashi

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