Laryngeal Cancer : in Relation to Oxidative Stress

This study was designed to investigate the oxidative stress parameters in laryngeal cancer and cancer-free adjacent tissues. Lipid peroxidation end product and the endogenous antioxidant components-CuZn superoxide dismutase (CuZn SOD) glutathione peroxidase (GSH Px), glutathione reductase (GSSG Rd) and glutathione (GSH)-were analysed by spectrophotometric and kinetic methods. Laryngeal cancer tissue exhibited higher lipid peroxidation than cancer free adjacent tissue. CuZn SOD and GSH Px activities and GSH level were significantly higher and GSSG Rd activity significantly lower in the cancer tissue. Detection of the antioxidant status may be useful to determine the tumour resistance to therapy, to choose the correct radiotherapy/chemotherapy and to monitor the effectiveness of the therapetic strategy.

[1]  T. Nose,et al.  Copper/Zinc Superoxide Dismutase, Nuclear DNA Content, and Progression in Human Gliomas , 1999, Journal of Neuro-Oncology.

[2]  L. Oberley,et al.  Antioxidant enzyme levels in oral squamous cell carcinoma and normal human oral epithelium. , 2002, Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology.

[3]  O. Yiğitbaşı,et al.  Oxidant and antioxidant status in larynx squamous cell carcinomas. , 2000, Journal of experimental & clinical cancer research : CR.

[4]  N. Korkut,et al.  Evaluation of oxidative stress parameters in blood of patients with laryngeal carcinoma. , 1999, Clinical biochemistry.

[5]  M. Samir,et al.  Thiobarbituric acid reactive substances in patients with laryngeal cancer. , 1999, Clinical otolaryngology and allied sciences.

[6]  Oberley Td,et al.  Antioxidant enzyme levels in cancer. , 1997 .

[7]  L. Oberley,et al.  Antioxidant enzyme levels in cancer. , 1997, Histology and histopathology.

[8]  J. Gutteridge,et al.  Lipid peroxidation and antioxidants as biomarkers of tissue damage. , 1995, Clinical chemistry.

[9]  H. Takase.  [Specificity of plasma peroxidized low density lipoprotein in lung cancer]. , 1995, Nihon Kyobu Shikkan Gakkai zasshi.

[10]  W. Peters,et al.  Glutathione S-transferases and glutathione in human head and neck cancer. , 1995, Carcinogenesis.

[11]  C. Enwonwu,et al.  Bionutrition and oral cancer in humans. , 1995, Critical Reviews in Oral Biology and Medicine.

[12]  W. Peters,et al.  Glutathione peroxidases in human head and neck cancer. , 1995, Acta oto-laryngologica.

[13]  C. Runowicz,et al.  Lipid-Soluble Antioxidants: β-Carotene and α-Tocopherol Levels in Breast and Gynecologic Cancers , 1994 .

[14]  J. Willson,et al.  Sensitive enzymatic cycling assay for glutathione: measurements of glutathione content and its modulation by buthionine sulfoximine in vivo and in vitro in human colon cancer. , 1994, Cancer research.

[15]  L. Loeb,et al.  Reactive oxygen species in tumorigenesis. , 1994, Cancer research.

[16]  M. Trush,et al.  Reactive oxygen-dependent DNA damage resulting from the oxidation of phenolic compounds by a copper-redox cycle mechanism. , 1994, Cancer research.

[17]  İ. Durak,et al.  Adenosine deaminase, 5'nucleotidase, xanthine oxidase, superoxide dismutase, and catalase activities in cancerous and noncancerous human bladder tissues. , 1993, Free radical biology & medicine.

[18]  C. Gokkusu,et al.  Lipid peroxidation and glutathione levels in patients with laryngeal carcinoma , 1994 .

[19]  C. Runowicz,et al.  Lipid-soluble antioxidants: beta-carotene and alpha-tocopherol levels in breast and gynecologic cancers. , 1994, Gynecologic oncology.

[20]  İ. Durak,et al.  Adenosine deaminase, 5' nucleotidase, xanthine oxidase, superoxide dismutase, and catalase activities in cancerous and noncancerous human laryngeal tissues. , 1994, Free radical biology & medicine.

[21]  Y. Yoshimura,et al.  Superoxide anion generation from peripheral blood neutrophils stimulated by N-formyl-methionyl-leucyl-phenylalanine in oromaxillary cancer patients. , 1993, Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons.

[22]  I. El-Dosoky,et al.  Elevation of glutathione, glutathione-reductase and nucleic acids in both normal tissues and tumour of breast cancer patients. , 1993, Cancer letters.

[23]  R. Perry,et al.  Glutathione levels and variability in breast tumors and normal tissue , 1993, Cancer.

[24]  C. Beauvillain de montreuil.  [Malignant tumors of the larynx and the hypopharynx. Epidemiology, diagnosis, treatment]. , 1993, La Revue du praticien.

[25]  N. Heintz,et al.  Differential regulation of antioxidant enzymes in response to oxidants. , 1991, The Journal of biological chemistry.

[26]  M. Thangaraju,et al.  Changes observed in antioxidant system in the blood of postmenopausal women with breast cancer. , 1991, Biochemistry international.

[27]  A. Krishan,et al.  Differential expression of glutathione S-transferase, glutathione peroxidase and glutathione reductase in normal and malignant human breast tissues. , 1990, Cancer letters.

[28]  Y. Sun,et al.  A simple method for clinical assay of superoxide dismutase. , 1988, Clinical chemistry.

[29]  P. Cerutti.  Prooxidant states and tumor promotion. , 1985, Science.

[30]  K. Yagi,et al.  Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. , 1979, Analytical biochemistry.

[31]  K. Ota,et al.  Increase of superoxide dismutase activity in various human leukemia cells. , 1979, Physiological chemistry and physics.

[32]  W. Valentine,et al.  Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. , 1967, The Journal of laboratory and clinical medicine.

[33]  E. Beutler,et al.  Improved method for the determination of blood glutathione. , 1963, The Journal of laboratory and clinical medicine.

[34]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.