The Association between Cardiorespiratory Fitness and Gut Microbiota Composition in Premenopausal Women

The aim of this study was to investigate the association between cardiorespiratory fitness and gut microbiota composition in premenopausal women. The participants consisted of 71 premenopausal Finnish women (aged 19–49 years). Gut microbiota were analyzed using flow cytometry, 16S rRNA gene hybridization and DNA-staining. Maximum oxygen uptake (VO2max) was assessed by respiratory gas analyzer and body composition by Bioimpdance. We found that participants with low VO2max had lower Bacteroides, but higher Eubacterium rectale-Clostridium coccoides than the high VO2max group (p < 0.05 for all). VO2max was inversely associated with EreC (r = −0.309, p = 0.01) but not with other bacteria. VO2max also negatively correlated with fat% (r = −0.755, p < 0.001), triglycerides (r = −0.274, p = 0.021) and leptin (r = −0.574, p < 0.001). By contrast, EreC was positively associated with fat% (r = 0.382, p = 0.002), dietary fat intake (r = 0.258, p = 0.034), triglycerides (r = 0.390, p = 0.002) and leptin (r = 0.424, p = 0.001), but negatively with carbohydrate intake (r = −0.252, p = 0.034) and HDL (r = −0.26, p = 0.028). After adjusting for age and dietary intake, all the significant associations remained. However, after adjusting for fat%, the associations between VO2max and EreC disappeared. Our results suggest that cardiorespiratory fitness is associated with gut microbiota composition, independent of age and carbohydrate or fat intake. The association between VO2max and EreC, however, appears to be mediated by body fatness.

[1]  M. Herbst-Kralovetz,et al.  Estrogen-gut microbiome axis: Physiological and clinical implications. , 2017, Maturitas.

[2]  S. Lynch,et al.  The Human Intestinal Microbiome in Health and Disease. , 2016, The New England journal of medicine.

[3]  N. Ajami,et al.  Gut Microbiome Associates With Lifetime Cardiovascular Disease Risk Profile Among Bogalusa Heart Study Participants. , 2016, Circulation research.

[4]  Sanjoy Ghosh,et al.  Cardiorespiratory fitness as a predictor of intestinal microbial diversity and distinct metagenomic functions , 2016, Microbiome.

[5]  D. Gevers,et al.  The Gut Microbiome Contributes to a Substantial Proportion of the Variation in Blood Lipids , 2015, Circulation research.

[6]  T. Törmäkangas,et al.  Adipose Tissue Dysfunction and Altered Systemic Amino Acid Metabolism Are Associated with Non-Alcoholic Fatty Liver Disease , 2015, PloS one.

[7]  I. Tarkka,et al.  Associations of disordered sleep with body fat distribution, physical activity and diet among overweight middle‐aged men , 2015, Journal of sleep research.

[8]  Jing Li,et al.  Dysbiosis gut microbiota associated with inflammation and impaired mucosal immune function in intestine of humans with non-alcoholic fatty liver disease , 2015, Scientific Reports.

[9]  K. Pietiläinen,et al.  Impact of a very low-energy diet on the fecal microbiota of obese individuals , 2014, European Journal of Nutrition.

[10]  S. Tuomela,et al.  Gut-adipose tissue axis in hepatic fat accumulation in humans. , 2014, Journal of hepatology.

[11]  E. Murphy,et al.  Exercise and associated dietary extremes impact on gut microbial diversity , 2014, Gut.

[12]  U. Kujala,et al.  Serum metabolic profiles in overweight and obese women with and without metabolic syndrome , 2014, Diabetology & Metabolic Syndrome.

[13]  Fredrik H. Karlsson,et al.  Understanding the interactions between bacteria in the human gut through metabolic modeling , 2013, Scientific Reports.

[14]  P. Bork,et al.  Richness of human gut microbiome correlates with metabolic markers , 2013, Nature.

[15]  Fredrik H. Karlsson,et al.  Gut metagenome in European women with normal, impaired and diabetic glucose control , 2013, Nature.

[16]  Lee M. Kaplan,et al.  Conserved Shifts in the Gut Microbiota Due to Gastric Bypass Reduce Host Weight and Adiposity , 2013, Science Translational Medicine.

[17]  P. Cotter,et al.  Role of the gut microbiota in health and chronic gastrointestinal disease: understanding a hidden metabolic organ , 2013, Therapeutic advances in gastroenterology.

[18]  M. Blaser,et al.  Antibiotics in early life alter the murine colonic microbiome and adiposity , 2012, Nature.

[19]  S. Keinänen-Kiukaanniemi,et al.  Women With and Without Metabolic Disorder Differ in Their Gut Microbiota Composition , 2012, Obesity.

[20]  P. O’Toole,et al.  Techniques used to characterize the gut microbiota: a guide for the clinician , 2012, Nature Reviews Gastroenterology &Hepatology.

[21]  J. Clemente,et al.  The Impact of the Gut Microbiota on Human Health: An Integrative View , 2012, Cell.

[22]  R. Lorenz,et al.  Gut Microbiota and Obesity , 2012, Current Obesity Reports.

[23]  Suzanne M. Paley,et al.  The MetaCyc database of metabolic pathways and enzymes and the BioCyc collection of pathway/genome databases , 2011, Nucleic Acids Res..

[24]  Wei Sun,et al.  The microbiome and butyrate regulate energy metabolism and autophagy in the mammalian colon. , 2011, Cell metabolism.

[25]  S. Sørensen,et al.  Gut Microbiota in Human Adults with Type 2 Diabetes Differs from Non-Diabetic Adults , 2010, PloS one.

[26]  A. Schwiertz,et al.  Microbiota and SCFA in Lean and Overweight Healthy Subjects , 2010, Obesity.

[27]  B. Roe,et al.  A core gut microbiome in obese and lean twins , 2008, Nature.

[28]  H. Flint,et al.  Human colonic microbiota associated with diet, obesity and weight loss , 2008, International Journal of Obesity.

[29]  P. Toivanen,et al.  Fecal microbiota in early rheumatoid arthritis. , 2008, The Journal of rheumatology.

[30]  H. Hara,et al.  Voluntary Running Exercise Alters Microbiota Composition and Increases n-Butyrate Concentration in the Rat Cecum , 2008, Bioscience, biotechnology, and biochemistry.

[31]  H. Flint,et al.  Reduced Dietary Intake of Carbohydrates by Obese Subjects Results in Decreased Concentrations of Butyrate and Butyrate-Producing Bacteria in Feces , 2006, Applied and Environmental Microbiology.

[32]  E. Mardis,et al.  An obesity-associated gut microbiome with increased capacity for energy harvest , 2006, Nature.

[33]  P. Turnbaugh,et al.  Microbial ecology: Human gut microbes associated with obesity , 2006, Nature.

[34]  P. Toivanen,et al.  Quantification of bacteria in human feces using 16S rRNA-hybridization, DNA-staining and flow cytometry. , 2005, Journal of microbiological methods.

[35]  V. Nouailhetas,et al.  Habitual exercise program protects murine intestinal, skeletal, and cardiac muscles against aging. , 2005, Journal of applied physiology.

[36]  F. Bäckhed,et al.  Obesity alters gut microbial ecology. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[37]  L. Kannas,et al.  Food consumption and nutrient intakes with a special focus on milk product consumption in early pubertal girls in Central Finland , 2005, Public Health Nutrition.

[38]  G. P. van Berge‐Henegouwen,et al.  Effects of regular physical activity on defecation pattern in middle-aged patients complaining of chronic constipation , 2005, Scandinavian journal of gastroenterology.

[39]  J. Doré,et al.  Fluorescent hybridisation combined with flow cytometry and hybridisation of total RNA to analyse the composition of microbial communities in human faeces using 16S rRNA probes. , 2003, FEMS microbiology ecology.

[40]  L. Akkermans,et al.  Potential benefits and hazards of physical activity and exercise on the gastrointestinal tract , 2001, Gut.

[41]  C. Gisolfi.  Is the GI System Built For Exercise? , 2000, News in physiological sciences : an international journal of physiology produced jointly by the International Union of Physiological Sciences and the American Physiological Society.

[42]  K. Schleifer,et al.  Phylogenetic identification and in situ detection of individual microbial cells without cultivation. , 1995, Microbiological reviews.

[43]  R. Amann,et al.  Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations , 1990, Applied and environmental microbiology.

[44]  D A Stahl,et al.  Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology , 1990, Journal of bacteriology.

[45]  M. Sjöström,et al.  Physical fitness in childhood and adolescence: a powerful marker of health , 2008, International Journal of Obesity.

[46]  J. Doré,et al.  Enumeration of Bacteroides species in human faeces by fluorescent in situ hybridisation combined with flow cytometry using 16S rRNA probes. , 2003, Systematic and applied microbiology.

[47]  P. D. di Prampero,et al.  The maximum oxygen intake. An international reference standard of cardiorespiratory fitness. , 1968, Bulletin of the World Health Organization.