Fabrication of precision scaffolds using liquid-frozen deposition manufacturing for cartilage tissue engineering.

The fused deposition manufacturing (FDM) system has been used to fabricate tissue-engineered scaffolds with highly interconnecting and controllable pore structure, although the system is limited to a few materials. For this reason, the liquid-frozen deposition manufacturing (LFDM) system based on an improvement of the FDM process was developed. Poly(D,L-lactide-co-glycolide) (PLGA) precision scaffolds were fabricated using LFDM from PLGA solutions of different concentrations. A greater concentration of PLGA solution resulted in greater mechanical strength but also resulted in less water content and smaller pore size on the surface of the scaffolds. LFDM scaffolds in general had mechanical strength closer to that of native articular cartilage than did FDM scaffolds. Neocartilage formation was observed in LFDM scaffolds seeded with porcine articular chondrocytes after 28 days of culture. Chondrocytes in LFDM scaffolds made from low concentrations (15-20%) of PLGA solution maintained a round shape, proliferated well, and secreted abundant extracellular matrix. In contrast, the FDM PLGA scaffolds had low cell numbers and poor matrix production because of heavy swelling. The LFDM system offered a useful way to fabricate scaffolds for cartilage tissue-engineering applications.

[1]  C. V. van Blitterswijk,et al.  The effect of PEGT/PBT scaffold architecture on the composition of tissue engineered cartilage. , 2005, Biomaterials.

[2]  Yongnian Yan,et al.  Fabrication of porous scaffolds for bone tissue engineering via low-temperature deposition , 2002 .

[3]  R. Ian Freshney,et al.  Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications , 2010 .

[4]  G. Naughton,et al.  Evaluation of matrix scaffolds for tissue engineering of articular cartilage grafts. , 1997, Journal of biomedical materials research.

[5]  J. Vacanti,et al.  A biodegradable nanofiber scaffold by electrospinning and its potential for bone tissue engineering. , 2003, Biomaterials.

[6]  Joo L. Ong,et al.  Diffusion in Musculoskeletal Tissue Engineering Scaffolds: Design Issues Related to Porosity, Permeability, Architecture, and Nutrient Mixing , 2004, Annals of Biomedical Engineering.

[7]  A. Mikos,et al.  Review: tissue engineering for regeneration of articular cartilage. , 2000, Biomaterials.

[8]  R Langer,et al.  Neocartilage formation in vitro and in vivo using cells cultured on synthetic biodegradable polymers. , 1993, Journal of biomedical materials research.

[9]  J Tramper,et al.  The effect of PEGT/PBT scaffold architecture on oxygen gradients in tissue engineered cartilaginous constructs. , 2004, Biomaterials.

[10]  R. Kandel,et al.  Effect of material geometry on cartilagenous tissue formation in vitro. , 2001, Journal of biomedical materials research.

[11]  A. Curtis,et al.  Articular chondrocyte passage number: Influence on adhesion, migration, cytoskeletal organisation and phenotype in response to nano‐ and micro‐metric topography , 2005, Cell biology international.

[12]  Han Tong Loh,et al.  Fabrication of 3D chitosan–hydroxyapatite scaffolds using a robotic dispensing system , 2002 .

[13]  D L Bader,et al.  Quantification of sulfated glycosaminoglycans in chondrocyte/alginate cultures, by use of 1,9-dimethylmethylene blue. , 1996, Analytical biochemistry.

[14]  C J Murphy,et al.  Effects of synthetic micro- and nano-structured surfaces on cell behavior. , 1999, Biomaterials.

[15]  I Zein,et al.  Mechanical properties and cell cultural response of polycaprolactone scaffolds designed and fabricated via fused deposition modeling. , 2001, Journal of biomedical materials research.

[16]  Shan-hui Hsu,et al.  Evaluation of the growth of chondrocytes and osteoblasts seeded into precision scaffolds fabricated by fused deposition manufacturing. , 2007, Journal of biomedical materials research. Part B, Applied biomaterials.

[17]  V. Goldberg,et al.  Repair of osteochondral defects with hyaluronan- and polyester-based scaffolds. , 2005, Osteoarthritis and cartilage.

[18]  D Amiel,et al.  The use of polylactic acid matrix and periosteal grafts for the reconstruction of rabbit knee articular defects. , 1991, Journal of biomedical materials research.

[19]  R. Landers,et al.  Rapid prototyping of scaffolds derived from thermoreversible hydrogels and tailored for applications in tissue engineering. , 2002, Biomaterials.

[20]  W. B. van den Berg,et al.  Crosslinked type II collagen matrices: preparation, characterization, and potential for cartilage engineering. , 2002, Biomaterials.

[21]  F M Watt,et al.  Influence of cytochalasin D-induced changes in cell shape on proteoglycan synthesis by cultured articular chondrocytes. , 1988, Experimental cell research.

[22]  C. Rorabeck,et al.  Increased damage to type II collagen in osteoarthritic articular cartilage detected by a new immunoassay. , 1994, The Journal of clinical investigation.

[23]  K. Leong,et al.  The design of scaffolds for use in tissue engineering. Part I. Traditional factors. , 2001, Tissue engineering.

[24]  J. Vacanti,et al.  Synthetic Polymers Seeded with Chondrocytes Provide a Template for New Cartilage Formation , 1991, Plastic and reconstructive surgery.

[25]  L. Shapiro,et al.  Novel alginate sponges for cell culture and transplantation. , 1997, Biomaterials.

[26]  Guoping Chen,et al.  Tissue engineering of cartilage using a hybrid scaffold of synthetic polymer and collagen. , 2004, Tissue engineering.

[27]  D. Hutmacher,et al.  Scaffolds in tissue engineering bone and cartilage. , 2000, Biomaterials.

[28]  Dietmar W Hutmacher,et al.  Repair of large articular osteochondral defects using hybrid scaffolds and bone marrow-derived mesenchymal stem cells in a rabbit model. , 2006, Tissue engineering.

[29]  Yong Wang,et al.  Adhesion and proliferation of OCT-1 osteoblast-like cells on micro- and nano-scale topography structured poly(L-lactide). , 2005, Biomaterials.

[30]  I. Martin,et al.  Producing prefabricated tissues and organs via tissue engineering , 1997, IEEE Engineering in Medicine and Biology Magazine.

[31]  F. Lin,et al.  Fabrication of viable tissue-engineered constructs with 3D cell-assembly technique. , 2005, Biomaterials.

[32]  S. Madihally,et al.  Porous chitosan scaffolds for tissue engineering. , 1999, Biomaterials.

[33]  S. Hsu,et al.  Evaluation of cellular affinity and compatibility to biodegradable polyesters and Type-II collagen-modified scaffolds using immortalized rat chondrocytes. , 2002, Artificial organs.

[34]  A. Grodzinsky,et al.  Fluorometric assay of DNA in cartilage explants using Hoechst 33258. , 1988, Analytical biochemistry.

[35]  Y. Lee,et al.  Response of human chondrocytes on polymer surfaces with different micropore sizes for tissue‐engineered cartilage , 2004 .

[36]  E. Kastenbauer,et al.  Cartilage tissue engineering with novel nonwoven structured biomaterial based on hyaluronic acid benzyl ester. , 1998, Journal of biomedical materials research.

[37]  C A van Blitterswijk,et al.  3D fiber-deposited scaffolds for tissue engineering: influence of pores geometry and architecture on dynamic mechanical properties. , 2006, Biomaterials.

[38]  H. Alexander,et al.  Effects of growth-factor-enhanced culture on a chondrocyte-collagen implant for cartilage repair. , 1996, Journal of biomedical materials research.

[39]  J. Mao,et al.  Structure and properties of bilayer chitosan-gelatin scaffolds. , 2003, Biomaterials.

[40]  A. Ratcliffe,et al.  Human articular chondrocyte adhesion and proliferation on synthetic biodegradable polymer films. , 1999, Biomaterials.