Scouting enzyme behavior

Experimental exploration of enzymatic response in a multidimensional context faces the challenge of an explosive number of possible milieu conditions. We address this problem with an evolutionary search strategy that scouts the physiochemical milieu space for unanticipated enzyme behavior and rewards the discovery of experimental conditions that yield surprises.

[1]  John K. Ousterhout,et al.  Tcl and the Tk Toolkit , 1994 .

[2]  M Conrad.  The seed germination model of enzyme catalysis. , 1992, Bio Systems.

[3]  Paul J. Layzell,et al.  Explorations in design space: unconventional electronics design through artificial evolution , 1999, IEEE Trans. Evol. Comput..

[4]  E. Freire,et al.  Statistical thermodynamic linkage between conformational and binding equilibria. , 1998, Advances in protein chemistry.

[5]  Klaus-Peter Zauner,et al.  Molecular approach to informal computing , 2001, Soft Comput..

[6]  V. L. Anderson.  Evolutionary Operation : A Method for Increasing Industrial Productivity , 1970 .

[7]  J. Ramsden,et al.  Evidence for Loosening of a Protein mechanism , 1998, Naturwissenschaften.

[8]  Ingo Rechenberg,et al.  Evolutionsstrategie : Optimierung technischer Systeme nach Prinzipien der biologischen Evolution , 1973 .

[9]  Marta Cascante,et al.  Effect of several anions on the activity of mitochondrial malate dehydrogenase from pig heart , 2001 .

[10]  A. Smith,et al.  Assay of serum NAD-dependent malate dehydrogenase using malate as substrate. , 1976, Clinica chimica acta; international journal of clinical chemistry.

[11]  M Conrad,et al.  Enzymatic Computing , 2001, Biotechnology progress.

[12]  Jeffrey O. Pfaffmann,et al.  Scouting context-sensitive components , 2001, Proceedings Third NASA/DoD Workshop on Evolvable Hardware. EH-2001.

[13]  D. Lindley.  On a Measure of the Information Provided by an Experiment , 1956 .

[14]  R. Hartley.  Transmission of information , 1928 .

[15]  B. Gontero,et al.  Memory and Imprinting in Multienzyme Complexes , 1999, The Journal of Biological Chemistry.

[16]  Sasha Englard,et al.  [21] Extramitochondrial l-malate dehydrogenase of beef heart: [EC 1.1.1.37 l-Malate: NAD oxidoreductase] , 1969 .

[17]  J L Gelpí,et al.  Kinetic studies of the regulation of mitochondrial malate dehydrogenase by citrate. , 1992, The Biochemical journal.

[18]  J M Yon,et al.  Conformational dynamics and enzyme activity. , 1998, Biochimie.

[19]  Felix T. Hong,et al.  Conformation-based molecular computing: simulation and implementation , 2001 .

[20]  George A. Miller,et al.  On Human Communication: A Review, a Survey, and a Criticism. , 1957 .

[21]  A. J. Mcevily,et al.  Regulation of mitochondrial malate dehydrogenase. Evidence for an allosteric citrate-binding site. , 1982, The Journal of biological chemistry.

[22]  J. King,et al.  Practical Clinical Enzymology , 1965 .

[23]  A. Sols,et al.  Multimodulation of enzyme activity. , 1981, Current topics in cellular regulation.