Biochip readout system for point-of-care applications

A new low cost biochip readout system based on total internal reflection fluorescence (TIRF) including microfluidics is presented. The system is suitable for point-of-care diagnostics employing disposable substrates made of polymer foils producible at very low costs. Results for the reader sensitivity and dynamic range in comparison to state of the art readers are presented. The sensitivity is comparable to laser scanner systems developed for lab applications, although the newly developed TIRF system is built up much simpler and low cost substrates are used. The dynamic range and the on chip homogeneity were investigated. As an example for future POC applications, an immunoassay for the inflammation parameter C-reactive protein (CRP) is investigated. The sensitivity of these assays is in the range of 1 ng/ml, being sufficient for many parameters of clinical relevance.

[1]  F. Bier,et al.  Real-time analysis on microarrays , 2004, Analytical and bioanalytical chemistry.

[2]  Markus H. Antwerpen,et al.  DNA microarray for detection of antibiotic resistance determinants in Bacillus anthracis and closely related Bacillus cereus. , 2007, Molecular and cellular probes.

[3]  N. Thompson,et al.  Total internal reflection fluorescence. , 1984, Annual review of biophysics and bioengineering.

[4]  Aigars Piruska,et al.  The autofluorescence of plastic materials and chips measured under laser irradiation. , 2005, Lab on a chip.

[5]  A. Fadeev,et al.  Trialkylsilane Monolayers Covalently Attached to Silicon Surfaces: Wettability Studies Indicating that Molecular Topography Contributes to Contact Angle Hysteresis , 1999 .

[6]  Alexander Y. Fadeev,et al.  Binary Monolayer Mixtures: Modification of Nanopores in Silicon-Supported Tris(trimethylsiloxy)silyl Monolayers , 1999 .

[7]  Katrin Schmitt,et al.  Direct detection of tuberculosis infection in blood serum using three optical label-free approaches , 2008 .

[8]  Jens Tschmelak,et al.  TIRF-based biosensor for sensitive detection of progesterone in milk based on ultra-sensitive progesterone detection in water , 2005, Analytical and bioanalytical chemistry.

[9]  O. Wolfbeis,et al.  Optical sensors: industrial environmental and diagnostic applications , 2004 .

[10]  C. Hoffmann,et al.  Mixed self-assembled monolayers (SAMs) consisting of methoxy-tri(ethylene glycol)-terminated and alkyl-terminated dimethylchlorosilanes control the non-specific adsorption of proteins at oxidic surfaces. , 2006, Journal of colloid and interface science.

[11]  G. Sulz,et al.  Real-time detection of nucleic acid interactions by total internal reflection fluorescence. , 2003, Analytical chemistry.

[12]  F. Bier,et al.  Label-free serodiagnosis on a grating coupler. , 2009, Methods in molecular biology.

[13]  Frances S. Ligler,et al.  TIRF Array Biosensor for Environmental Monitoring , 2004 .

[14]  Frieder W Scheller,et al.  Detection of progesterone in whole blood samples. , 2003, Biosensors & bioelectronics.

[15]  J. Lakowicz.  Topics in fluorescence spectroscopy , 2002 .

[16]  M. A. Bopp,et al.  Zeptosens' protein microarrays: A novel high performance microarray platform for low abundance protein analysis , 2002, Proteomics.

[17]  Frances S. Ligler,et al.  An Array Immunosensor for Simultaneous Detection of Clinical Analytes , 1999 .