Microfluidic Platform for Enzyme-Linked and Magnetic Particle-Based Immunoassay

This article presents design and testing of a microfluidic platform for immunoassay. The method is based on sandwiched ELISA, whereby the primary antibody is immobilized on nitrocelluose and, subsequently, magnetic beads are used as a label to detect the analyte. The chip takes approximately 2 h and 15 min to complete the assay. A Hall Effect sensor using 0.35-μm BioMEMS TSMC technology (Taiwan Semiconductor Manufacturing Company Bio-Micro-Electro-Mechanical Systems) was fabricated to sense the magnetic field from the beads. Furthermore, florescence detection and absorbance measurements from the chip demonstrate successful immunoassay on the chip. In addition, investigation also covers the Hall Effect simulations, mechanical modeling of the bead-protein complex, testing of the microfluidic platform with magnetic beads averaging 10 nm, and measurements with an inductor-based system.

[1]  R. Fair,et al.  Droplet-based microfluidic lab-on-a-chip for glucose detection , 2004 .

[2]  Yen-Heng Lin,et al.  Integrating solid-state sensor and microfluidic devices for glucose, urea and creatinine detection based on enzyme-carrying alginate microbeads. , 2013, Biosensors & bioelectronics.

[3]  Reinhard Niessner,et al.  Multiplexed electrochemical immunoassay of biomarkers using metal sulfide quantum dot nanolabels and trifunctionalized magnetic beads. , 2013, Biosensors & bioelectronics.

[4]  Giovanna Marrazza,et al.  Electrochemical detection of miRNA-222 by use of a magnetic bead-based bioassay , 2012, Analytical and Bioanalytical Chemistry.

[5]  Y. Terauchi,et al.  The fat-derived hormone adiponectin reverses insulin resistance associated with both lipoatrophy and obesity , 2001, Nature Medicine.

[6]  A. Helmicki,et al.  An integrated microfluidic biochemical detection system for protein analysis with magnetic bead-based sampling capabilities. , 2002, Lab on a chip.

[7]  R. Fair,et al.  An integrated digital microfluidic lab-on-a-chip for clinical diagnostics on human physiological fluids. , 2004, Lab on a chip.

[8]  Leslie Y Yeo,et al.  Microfluidic devices for bioapplications. , 2011, Small.

[9]  An-Yu Chang,et al.  A CMOS magnetic microbead-based capacitive biosensor array with on-chip electromagnetic manipulation. , 2013, Biosensors & bioelectronics.

[10]  B. Boser,et al.  Fully integrated detection of single magnetic beads in complementary metal-oxide-semiconductor , 2008 .

[11]  Mwj Menno Prins,et al.  Rapid integrated biosensor for multiplexed immunoassays based on actuated magnetic nanoparticles. , 2009, Lab on a chip.

[12]  B. Boser,et al.  On-chip magnetic separation of superparamagnetic beads for integrated molecular analysis. , 2010, Journal of applied physics.

[13]  Guonan Chen,et al.  DNAzyme-based magneto-controlled electronic switch for picomolar detection of lead (II) coupling with DNA-based hybridization chain reaction. , 2013, Biosensors & bioelectronics.

[14]  Richard B. Fair,et al.  Digital microfluidics: is a true lab-on-a-chip possible? , 2007 .

[15]  Chuan-Hua Zhou,et al.  A magnetic bead-based bienzymatic electrochemical immunosensor for determination of H9N2 avian influenza virus , 2013 .

[16]  Y. K. Cheung,et al.  1 Supplementary Information for : Microfluidics-based diagnostics of infectious diseases in the developing world , 2011 .

[17]  Gwo-Bin Lee,et al.  An integrated microfluidic platform for rapid tumor cell isolation, counting and molecular diagnosis , 2013, Biomedical Microdevices.

[18]  S. V. Kergaravat,et al.  Electrochemical magneto immunosensor for the detection of anti-TG2 antibody in celiac disease. , 2013, Biosensors & bioelectronics.

[19]  A. Lu,et al.  Magnetic nanoparticles: synthesis, protection, functionalization, and application. , 2007, Angewandte Chemie.

[20]  Mingyu Lu,et al.  Focusing Light with Curved Guided-Mode Resonance Reflectors , 2011, Micromachines.

[21]  B. Boser,et al.  A novel magnetic bead bioassay platform using a microchip-based sensor for infectious disease diagnosis. , 2006, Journal of immunological methods.

[22]  Peter J. Hesketh,et al.  Microbeads for Sampling and Mixing in a Complex Sample , 2013, Micromachines.