Combination of 1H NMR and chemometrics to discriminate manuka honey from other floral honey types from Oceania.

Manuka honey is a product produced essentially in New Zealand, and has been widely recognised for its antibacterial properties and specific taste. In this study, 264 honeys from New Zealand and Australia were analysed using proton NMR spectroscopy coupled with chemometrics. Known manuka markers, methylglyoxal and dihydroxyacetone, have been characterised and quantified, together with a new NMR marker, identified as being leptosperin. Manuka honey profiling using 1H NMR is shown to be a possible alternative to chromatography with the added advantage that it can measure methylglyoxal (MGO), dihydroxyacetone (DHA) and leptosperin simultaneously. By combining the information from these three markers, we established a model to estimate the proportion of manuka in a given honey. Markers of other botanical origins were also identified, which makes 1H NMR a convenient and efficient tool, complementary to pollen analysis, to control the botanical origin of Oceania honeys.

[1]  M. Fielder,et al.  The application of high resolution diffusion NMR to the analysis of manuka honey. , 2012, Food chemistry.

[2]  D. Jouan-Rimbaud Bouveresse,et al.  Two novel methods for the determination of the number of components in independent components analysis models , 2012 .

[3]  Steven J Lehotay,et al.  Determination of pesticide residues in foods by acetonitrile extraction and partitioning with magnesium sulfate: collaborative study. , 2007, Journal of AOAC International.

[4]  D. Lachenmeier,et al.  Qualitative and Quantitative Control of Honeys Using NMR Spectroscopy and Chemometrics , 2013 .

[5]  Vincent Mazet,et al.  Background removal from spectra by designing and minimising a non-quadratic cost function , 2005 .

[6]  F Savorani,et al.  icoshift: A versatile tool for the rapid alignment of 1D NMR spectra. , 2010, Journal of magnetic resonance.

[7]  A. Charlton,et al.  Quantitative NMR spectroscopy for the rapid measurement of methylglyoxal in manuka honey , 2010 .

[8]  K. Speer,et al.  Differentiation of manuka honey from kanuka honey and from jelly bush honey using HS-SPME-GC/MS and UHPLC-PDA-MS/MS. , 2014, Journal of agricultural and food chemistry.

[9]  Christophe Junot,et al.  High-resolution mass spectrometry associated with data mining tools for the detection of pollutants and chemical characterization of honey samples. , 2014, Journal of agricultural and food chemistry.

[10]  D. Jouan-Rimbaud Bouveresse,et al.  Independent components analysis with the JADE algorithm , 2012 .

[11]  K. Rogers,et al.  The unique manuka effect: why New Zealand manuka honey fails the AOAC 998.12 C-4 sugar method. , 2014, Journal of agricultural and food chemistry.

[12]  H. Kikuzaki,et al.  Identification of a novel glycoside, leptosin, as a chemical marker of manuka honey. , 2012, Journal of agricultural and food chemistry.

[13]  Douglas N Rutledge,et al.  Fast and global authenticity screening of honey using ¹H-NMR profiling. , 2015, Food chemistry.

[14]  Liam G. Fearnley,et al.  Phenolic compounds and methylglyoxal in some New Zealand manuka and kanuka honeys. , 2010 .

[15]  M. Manley-Harris,et al.  The origin of methylglyoxal in New Zealand manuka (Leptospermum scoparium) honey. , 2009, Carbohydrate research.

[16]  Benjamin J. Deadman,et al.  Isolation by HPLC and characterisation of the bioactive fraction of New Zealand manuka (Leptospermum scoparium) honey. , 2008, Carbohydrate research.

[17]  Y. Nitta,et al.  Plausible authentication of manuka honey and related products by measuring leptosperin with methyl syringate. , 2014, Journal of agricultural and food chemistry.