Lipase-catalyzed methanolysis of microalgae oil for biodiesel production and PUFAs concentration

Abstract A novel process with the combined use of lipase NS81006 and Novozym435 was developed for the conversion of microalgae oils for biodiesel production and PUFAs concentration. It was found that during the first-step reaction catalyzed by NS81006, the reaction rates of PUFAs were much slower compared to those with carbon length varying from C14 to C18, but significant increase for PUFAs' conversion was achieved with Novozym435 as the catalyst for the second step conversion. A fatty acid methyl ester (FAME) yield of 95% could be obtained with this two-step enzymatic catalysis. This process has great prospect for converting microalgae oils for biodiesel preparation and PUFAs concentration.

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