Cytological observations on the strandedness of mammalian metaphase chromosomes.

Although much is known about the roles and behavior of the chemical components of chromosomes, virtually no information is available as to how these chemical components are organized in the chromosome. The delineation of this organization will help in un derstanding genetic and cytogenetic functions of the chromosome. A prerequisite for the analysis of chromosome structure and chemistry is an easily obtained source of unaltered chromosomes that can be visibly identified. Although it has been possible to isolate meta phase chromosomes, the techniques were either laborious or gave rise to altered chromo somes (Chorazy, Bendich, Borenfreund and Hutchison 1963, Prescott and Bender 1961, Somers, Cole and Hsu 1963). Furthermore, previous studies on chromosome chemistry have been carried out on chromosomes that are not visibly identifiable (Dounce and Hilgartner 1964, Mirsky and Ris 1947-1948). This report describes cytological observations on the strandedness of unfixed metaphase chromosomes isolated by a simple technique.

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