The Septins Are Required for the Mitosis-specific Activation of the Gin4 Kinase

In budding yeast, a protein kinase called Gin4 is specifically activated during mitosis and functions in a pathway initiated by the Clb2 cyclin to control bud growth. We have used genetics and biochemistry to identify additional proteins that function with Gin4 in this pathway, and both of these approaches have identified members of the septin family. Loss of septin function produces a phenotype that is very similar to the phenotype caused by loss of Gin4 function, and the septins are required early in mitosis to activate Gin4 kinase activity. Furthermore, septin mutants display a prolonged mitotic delay at the short spindle stage, consistent with a role for the septins in the control of mitotic events. Members of the septin family bind directly to Gin4, demonstrating that the functions of Gin4 and the septins must be closely linked within the cell. These results demonstrate that the septins in budding yeast play an integral role in the mitosis-specific regulation of the Gin4 kinase and that they carry out functions early in mitosis.

[1]  M. Longtine,et al.  Role of the Yeast Gin4p Protein Kinase in Septin Assembly and the Relationship between Septin Assembly and Septin Function , 1998, The Journal of cell biology.

[2]  M. Mann,et al.  Polymerization of Purified Yeast Septins: Evidence That Organized Filament Arrays May Not Be Required for Septin Function , 1998, The Journal of cell biology.

[3]  Claudio De Virgilio,et al.  A Septin-based Hierarchy of Proteins Required for Localized Deposition of Chitin in the Saccharomyces cerevisiae Cell Wall , 1997, The Journal of cell biology.

[4]  R. Altman,et al.  Control of Mitotic Events by Nap1 and the Gin4 Kinase , 1997, The Journal of cell biology.

[5]  Y. Hiraoka,et al.  Nedd5, a mammalian septin, is a novel cytoskeletal component interacting with actin-based structures. , 1997, Genes & development.

[6]  D. Kiehart,et al.  Septins may form a ubiquitous family of cytoskeletal filaments , 1996, The Journal of cell biology.

[7]  B. Alberts,et al.  A purified Drosophila septin complex forms filaments and exhibits GTPase activity , 1996, The Journal of cell biology.

[8]  A. Shevchenko,et al.  Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels. , 1996, Analytical chemistry.

[9]  M. Longtine,et al.  The septins: roles in cytokinesis and other processes. , 1996, Current opinion in cell biology.

[10]  J. Pringle,et al.  Identification of a developmentally regulated septin and involvement of the septins in spore formation in Saccharomyces cerevisiae , 1996, The Journal of cell biology.

[11]  J. Pringle,et al.  Localization and possible functions of Drosophila septins. , 1995, Molecular biology of the cell.

[12]  M. Bhattacharyya,et al.  The SPR3 gene encodes a sporulation-specific homologue of the yeast CDC3/10/11/12 family of bud neck microfilaments and is regulated by ABFI. , 1995, Gene.

[13]  A. Murray,et al.  NAP1 acts with Clb1 to perform mitotic functions and to suppress polar bud growth in budding yeast , 1995, The Journal of cell biology.

[14]  A. Murray,et al.  Members of the NAP/SET family of proteins interact specifically with B- type cyclins , 1995, The Journal of cell biology.

[15]  I. Herskowitz,et al.  Role of Bud3p in producing the axial budding pattern of yeast , 1995, The Journal of cell biology.

[16]  S. Reed,et al.  A cell cycle checkpoint monitors cell morphogenesis in budding yeast , 1995, The Journal of cell biology.

[17]  S. Reed,et al.  Cell cycle control of morphogenesis in budding yeast. , 1995, Current opinion in genetics & development.

[18]  J. Yates,et al.  An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein database , 1994, Journal of the American Society for Mass Spectrometry.

[19]  C. Field,et al.  Cell Division: Septins in common? , 1994, Current Biology.

[20]  T. P. Neufeld,et al.  The Drosophila peanut gene is required for cytokinesis and encodes a protein similar to yeast putative bud neck filament proteins , 1994, Cell.

[21]  K. Nasmyth,et al.  Dams and sluices , 1993, Nature.

[22]  K. Nasmyth,et al.  Yeast G1 cyclins CLN1 and CLN2 and a GAP‐like protein have a role in bud formation. , 1993, The EMBO journal.

[23]  F. Cross,et al.  Genetic analysis of Cln/Cdc28 regulation of cell morphogenesis in budding yeast. , 1993, The EMBO journal.

[24]  M. Snyder,et al.  Components required for cytokinesis are important for bud site selection in yeast , 1993, The Journal of cell biology.

[25]  J. Pines,et al.  Cyclins and cyclin-dependent kinases: take your partners , 1993 .

[26]  S. Reed,et al.  Morphogenesis in the yeast cell cycle: regulation by Cdc28 and cyclins , 1993, The Journal of cell biology.

[27]  B. Haarer,et al.  Cellular morphogenesis in the Saccharomyces cerevisiae cell cycle: localization of the CDC3 gene product and the timing of events at the budding site , 1991, The Journal of cell biology.

[28]  Frank McCormick,et al.  The GTPase superfamily: conserved structure and molecular mechanism , 1991, Nature.

[29]  J. Jorgenson,et al.  Preparation and evaluation of packed capillary liquid chromatography columns with inner diameters from 20 to 50 μm , 1989 .

[30]  J. Jorgenson,et al.  Quantitative analysis of individual neurons by open tubular liquid chromatography with voltammetric detection. , 1989, Analytical chemistry.

[31]  B. Haarer,et al.  Immunofluorescence localization of the Saccharomyces cerevisiae CDC12 gene product to the vicinity of the 10-nm filaments in the mother-bud neck , 1987, Molecular and cellular biology.

[32]  D. Lowy,et al.  A transforming ras gene can provide an essential function ordinarily supplied by an endogenous ras gene , 1986, Molecular and cellular biology.

[33]  B. Carter,et al.  Genetic control of cell division in yeast cultured at different growth rates , 1977, Nature.

[34]  B. Byers,et al.  A highly ordered ring of membrane-associated filaments in budding yeast , 1976, The Journal of cell biology.

[35]  L. Hartwell,et al.  Genetic control of the cell division cycle in yeast. , 1974, Science.

[36]  L. Hartwell.  Genetic control of the cell division cycle in yeast. IV. Genes controlling bud emergence and cytokinesis. , 1971, Experimental cell research.

[37]  L. Hartwell,et al.  Genetic control of the cell-division cycle in yeast. I. Detection of mutants. , 1970, Proceedings of the National Academy of Sciences of the United States of America.

[38]  J. Yates,et al.  Identifying the major proteome components of Haemophilus influenzae type‐strain NCTC 8143 , 1997, Electrophoresis.

[39]  J. E. Celis,et al.  Cell Biology: A Laboratory Handbook , 1997 .

[40]  B. Alberts,et al.  Purification of a multiprotein complex containing centrosomal proteins from the Drosophila embryo by chromatography with low-affinity polyclonal antibodies. , 1992, Molecular biology of the cell.

[41]  B. Haarer,et al.  Immunofluorescence methods for yeast. , 1991, Methods in enzymology.

[42]  Susan K. Ford,et al.  Cellular morphogenesis in the Saccharomyces cerevisiae cell cycle: localization of the CDC11 gene product and the timing of events at the budding site. , 1991, Developmental genetics.