Residue depletion of oxytetracycline (OTC) and 4-epi-oxytetracycline (4-epi-OTC) in broiler chicken’s claws by liquid chromatography-tandem mass spectrometry (LC-MS/MS)

ABSTRACT Antibiotics are widely used in poultry production for the treatment of bacterial diseases. However, residues may remain in products and by-products destined for human consumption or animal feeding. The claws of chickens, which are a by-product of the poultry industry, can directly or indirectly enter the food chain as meals destined to feed other productive animals. Thus, it becomes necessary to determine and quantify antimicrobial residues present in this matrix. The objective of the study was to assess the depletion of oxytetracycline (OTC) and its metabolite 4-epi-OTC in broiler chicken’s claws. Claws of 32 broilers treated with a therapeutic dosage of 10% OTC during 7 days were analysed. Samples were taken at days 3, 9, 15 and 19 post-treatment. As for the control group, eight broiler chickens were raised under the same conditions. Extraction was carried out through EDTA-McIlvaine buffer, and clean-up employed a SPE C-18 Sep-Pak®. Instrumental analysis was performed through LC-MS/MS. The concentrations of both analytes were determined in claw samples until day 19 post-treatment. Average concentrations were within the LOD (20 μg kg–1) and LOQ (22 µg kg–1) for OTC and 84 μg kg–1 for 4-epi-OTC. Withdrawal times (WDTs) of 39 days for OTC and 54 days for 4-epi-OTC were established in claws based on 95% confidence. These findings demonstrate that claws can be a source of antimicrobial residue entry into the food chain, since the results showed that OTC and its metabolite can be found in chicken’s claws for long periods, even exceeding the average lifespan of a broiler chicken. Graphical Abstract

[1]  J. Cornejo,et al.  Assessment of Three Antimicrobial Residue Concentrations in Broiler Chicken Droppings as a Potential Risk Factor for Public Health and Environment , 2018, International journal of environmental research and public health.

[2]  J. Cornejo,et al.  Residue Depletion of Florfenicol and Florfenicol Amine in Broiler Chicken Claws and a Comparison of Their Concentrations in Edible Tissues Using LC–MS/MS , 2018, Molecules.

[3]  L. Lapierre,et al.  Determination of Chlortetracycline Residues, Antimicrobial Activity and Presence of Resistance Genes in Droppings of Experimentally Treated Broiler Chickens , 2018, Molecules.

[4]  J. Rodríguez,et al.  Solid-Phase Extraction and Large-Volume Sample Stacking-Capillary Electrophoresis for Determination of Tetracycline Residues in Milk , 2018, Journal of analytical methods in chemistry.

[5]  V. Meucci,et al.  Cytotoxic effects of oxytetracycline residues in the bones of broiler chickens following therapeutic oral administration of a water formulation , 2015, Poultry science.

[6]  B. Berendsen,et al.  The disposition of oxytetracycline to feathers after poultry treatment , 2013, Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment.

[7]  M. Sharman,et al.  Can the unauthorised use of ceftiofur be detected in poultry? , 2013, Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment.

[8]  A. Anadón,et al.  Plasma disposition and tissue depletion of chlortetracycline in the food producing animals, chickens for fattening. , 2012, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[9]  R. Halden,et al.  Feather meal: a previously unrecognized route for reentry into the food supply of multiple pharmaceuticals and personal care products (PPCPs). , 2012, Environmental science & technology.

[10]  P. González,et al.  Transfer and depletion of enrofloxacin and its metabolite ciprofloxacin in feathers of treated broiler chickens. [Conference poster]. , 2012 .

[11]  L. Lapierre,et al.  Depletion study of three formulations of flumequine in edible tissues and drug transfer into chicken feathers. , 2011, Journal of veterinary pharmacology and therapeutics.

[12]  F. André,et al.  Withdrawal time of four pharmaceutical formulations of enrofloxacin in poultry according to different maximum residues limits. , 2009, Journal of veterinary pharmacology and therapeutics.

[13]  M. Castellari,et al.  Detection of tetracycline and oxytetracycline residues in pig and calf hair by ultra-high-performance liquid chromatography tandem mass spectrometry. , 2009, Journal of chromatography. A.

[14]  C. Méndez,et al.  Determination of oxytetracycline residues in samples of bovine tissues Used for human consumption , 2008 .

[15]  S. Bronzwaer.  Harmonised monitoring of antimicrobial resistance in Salmonella and Campylobacter isolates from food animals in the European Union. , 2008, Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases.

[16]  A. Anadón,et al.  Depletion study of enrofloxacin and its metabolite ciprofloxacin in edible tissues and feathers of white leghorn hens by liquid chromatography coupled with tandem mass spectrometry. , 2007, Journal of food protection.

[17]  Arturo Anadón Navarro,et al.  Residuos de medicamentos de uso veterinario , 2006 .

[18]  U. Brinkman,et al.  Analytical strategies for residue analysis of veterinary drugs and growth-promoting agents in food-producing animals--a review. , 2005, Journal of chromatography. A.

[19]  P. Guy,et al.  Analysis of tetracyclines in honey by high-performance liquid chromatography/tandem mass spectrometry. , 2005, Rapid communications in mass spectrometry : RCM.

[20]  D. Donoghue,et al.  Multiresidue determination of fluoroquinolone antibiotics in eggs using liquid chromatography-fluorescence-mass spectrometryn , 2003 .

[21]  M. Kühne,et al.  Tetracycline residues in meat and bone meals. Part 2: The effect of heat treatments on bound tetracycline residues , 2001, Food additives and contaminants.

[22]  S. Wenzel,et al.  Tetracycline residues in meat and bone meals. Part 1: Methodology and examination of field samples , 2001, Food additives and contaminants.

[23]  Dimitrios Fletouris,et al.  Drug Residues in Foods : Pharmacology: Food Safety, and Analysis , 2000 .