Surface Passivation of Carbon Nanoparticles with Branched Macromolecules Influences Near Infrared Bioimaging

A superior and commercially exploitable 'green synthesis' of optically active carbon nanoparticle (OCN) is revealed in this work. The naked carbon particles (<20 nm) were derived from commercial food grade honey. The fluorescence properties of these particles were significantly enhanced by utilizing hyberbranched polymer for surface passivation. A dramatic increase in near infrared emission was achieved compared to a linear polymer (PEG) coated carbon nanoparticles. Interestingly, as passivating agent becomes more extensively branched (pseudo generation 2 to 4), the average radiant efficiency amplifies considerably as a direct result of the increasing surface area available for light passivation. The particles showed negligible loss of cell viability in presence of endothelial cells in vitro. Preliminary in vivo experiment showed high contrast enhancement in auxiliary lymphnode in a mouse model. The exceptionally rapid lymphatic transport of these particles suggests that such an approach may offer greater convenience and reduced procedural expense, as well as improved surgical advantage as the patient is positioned on the table for easier resection.

[1]  Guangxia Shen,et al.  Light‐Triggered Theranostics Based on Photosensitizer‐Conjugated Carbon Dots for Simultaneous Enhanced‐Fluorescence Imaging and Photodynamic Therapy , 2012, Advanced materials.

[2]  Xin Cai,et al.  A green synthesis of carbon nanoparticles from honey and their use in real-time photoacoustic imaging , 2013, Nano Research.

[3]  Haifang Wang,et al.  Fluorescent carbon dots and nanodiamonds for biological imaging: preparation, application, pharmacokinetics and toxicity. , 2012, Current drug metabolism.

[4]  Wei Cai,et al.  Size-tunable synthesis of monodisperse water-soluble gold nanoparticles with high X-ray attenuation. , 2010, Chemistry.

[5]  Xing-Jie Liang,et al.  Nano-Carbons as Theranostics , 2012, Theranostics.

[6]  Vasilis Ntziachristos,et al.  Deep tissue optical and optoacoustic molecular imaging technologies for pre-clinical research and drug discovery. , 2012, Current pharmaceutical biotechnology.

[7]  Ya‐Ping Sun,et al.  Quantum-sized carbon dots for bright and colorful photoluminescence. , 2006, Journal of the American Chemical Society.

[8]  Sheila N. Baker,et al.  Luminescent carbon nanodots: emergent nanolights. , 2010, Angewandte Chemie.

[9]  C. Mao,et al.  Fluorescent carbon nanoparticles derived from candle soot. , 2007, Angewandte Chemie.

[10]  Hak Soo Choi,et al.  Targeted zwitterionic near-infrared fluorophores for improved optical imaging , 2013, Nature Biotechnology.

[11]  Ya‐Ping Sun,et al.  Bandgap-like strong fluorescence in functionalized carbon nanoparticles. , 2010, Angewandte Chemie.

[12]  K. Choi,et al.  A facile, one-step nanocarbon functionalization for biomedical applications. , 2012, Nano letters.

[13]  D. Andrews,et al.  An Introduction to Laser Spectroscopy , 1996 .

[14]  Manojit Pramanik,et al.  Near infrared photoacoustic detection of sentinel lymph nodes with gold nanobeacons. , 2010, Biomaterials.

[15]  Debraj Ghosh,et al.  Nanosized Carbon Particles From Natural Gas Soot , 2009 .

[16]  Lihong V. Wang,et al.  A Green Synthesis of Carbon Nanoparticle from Honey for Real-Time Photoacoustic Imaging. , 2013, Nano research.

[17]  K. Loh,et al.  One-pot synthesis of fluorescent carbon nanoribbons, nanoparticles, and graphene by the exfoliation of graphite in ionic liquids. , 2009, ACS nano.

[18]  Lei Tao,et al.  Biocompatible polydopamine fluorescent organic nanoparticles: facile preparation and cell imaging. , 2012, Nanoscale.

[19]  Lei Tao,et al.  Fluoridated HAp:Ln3+ (Ln = Eu or Tb) nanoparticles for cell-imaging. , 2012, Nanoscale.

[20]  S. Achilefu,et al.  Fluorescence lifetime measurements and biological imaging. , 2010, Chemical reviews.

[21]  K. Chattopadhyay,et al.  A facile synthesis of PEG-coated magnetite (Fe3O4) nanoparticles and their prevention of the reduction of cytochrome c. , 2012, ACS applied materials & interfaces.

[22]  W. Shi,et al.  Synthesis and characterization of ZnS/hyperbranched polyester nanocomposite and its optical properties , 2007 .

[23]  Dhiraj Kumar,et al.  Polyethylene glycol functionalized gold nanoparticles: the influence of capping density on stability in various media , 2011 .

[24]  Minghong Wu,et al.  Controlled synthesis of green and blue luminescent carbon nanoparticles with high yields by the carbonization of sucrose , 2010 .

[25]  Hui Peng,et al.  Simple Aqueous Solution Route to Luminescent Carbogenic Dots from Carbohydrates , 2009 .

[26]  V. Ntziachristos.  Going deeper than microscopy: the optical imaging frontier in biology , 2010, Nature Methods.

[27]  Zhikun Wu,et al.  Quantum sized gold nanoclusters with atomic precision. , 2012, Accounts of chemical research.

[28]  Ben Zhong Tang,et al.  Biocompatible Nanoparticles with Aggregation‐Induced Emission Characteristics as Far‐Red/Near‐Infrared Fluorescent Bioprobes for In Vitro and In Vivo Imaging Applications , 2012 .

[29]  Kai Yang,et al.  In vivo NIR fluorescence imaging, biodistribution, and toxicology of photoluminescent carbon dots produced from carbon nanotubes and graphite. , 2012, Small.

[30]  Wei Cai,et al.  Luminescent Au11 nanocluster superlattices with high thermal stability , 2012 .

[31]  Li Cao,et al.  Competitive Performance of Carbon “Quantum” Dots in Optical Bioimaging , 2012, Theranostics.

[32]  Lei Tao,et al.  Carbon-dots derived from nanodiamond: photoluminescence tunable nanoparticles for cell imaging. , 2013, Journal of colloid and interface science.

[33]  Chunhai Fan,et al.  The Biocompatibility of Nanodiamonds and Their Application in Drug Delivery Systems , 2012, Theranostics.

[34]  Jing Li,et al.  One-pot green synthesis of optically pH-sensitive carbon dots with upconversion luminescence. , 2012, Nanoscale.

[35]  Kai Yang,et al.  Carbon materials for drug delivery & cancer therapy , 2011 .

[36]  Nikhil R. Jana,et al.  Carbon Nanoparticle-based Fluorescent Bioimaging Probes , 2013, Scientific Reports.

[37]  Magdalena Swierczewska,et al.  The design and application of fluorophore-gold nanoparticle activatable probes. , 2011, Physical chemistry chemical physics : PCCP.

[38]  Lei Tao,et al.  Surfactant modification of aggregation-induced emission material as biocompatible nanoparticles: facile preparation and cell imaging. , 2013, Nanoscale.