Effect of steam explosion and microbial fermentation on cellulose and lignin degradation of corn stover.

In order to increase nutrient values of corn stover, effects of steam explosion (2.5 MPa, 200 s) and Aspergillus oryzae (A. oryzae) fermentation on cellulose and lignin degradation were studied. The results showed the contents of cellulose, hemicellulose and lignin in the exploded corn stover were 8.47%, 50.45% and 36.65% lower than that in the untreated one, respectively (P<0.05). The contents of cellulose and hemicellulose in the exploded and fermented corn stover (EFCS) were decreased by 24.36% and 69.90%, compared with the untreated one (P<0.05); decreased by 17.35% and 38.59%, compared with the exploded one (P<0.05). The scanning electron microscope observations demonstrated that the combined steam explosion and fermentation destructed corn stover. The activities of enzymes in EFCS were increased. The metabolic experiment showed that about 8% EFCS could be used to replace corn meal in broiler diets, which made EFCS become animal feedstuff possible.

[1]  Lirong Ma,et al.  Utilization of straw in biomass energy in China , 2007 .

[2]  M. Himmel,et al.  Deposition of Lignin Droplets Produced During Dilute Acid Pretreatment of Maize Stems Retards Enzymatic Hydrolysis of Cellulose , 2007, Biotechnology progress.

[3]  J. Shih,et al.  Effects of dietary supplementation of keratinase on growth performance, nitrogen retention and intestinal morphology of broiler chickens fed diets with soybean and cottonseed meals ☆ , 2008 .

[4]  M. Himmel,et al.  Welcome to Biotechnology for Biofuels , 2008, Biotechnology for biofuels.

[5]  K. Beauchemin,et al.  Effects of an enzyme feed additive on extent of digestion and milk production of lactating dairy cows. , 1999, Journal of dairy science.

[6]  Ashok Pandey,et al.  Comparative evaluation of neutral protease production by Aspergillus oryzae in submerged and solid-state fermentation , 2005 .

[7]  Q. Guan,et al.  Effect of the Transformed Lactobacillus with Phytase Gene on Pig Production Performance, Nutrient Digestibility, Gut Microbes and Serum Biochemical Indexes , 2009 .

[8]  G. Zeng,et al.  Biodelignification of rice straw by Phanerochaete chrysosporium in the presence of dirhamnolipid , 2010, Biodegradation.

[9]  P. Thonart,et al.  Isolation and identification of a new fungal strain for amylase biosynthesis. , 2009, Polish journal of microbiology.

[10]  T. K. Ghose Measurement of cellulase activities , 1987 .

[11]  Rui M. F. Bezerra,et al.  Modification of wheat straw lignin by solid state fermentation with white-rot fungi. , 2009, Bioresource technology.

[12]  Lijun Wang,et al.  Effect of steam explosion on biodegradation of lignin in wheat straw. , 2008, Bioresource technology.

[13]  R. Blaser Symposium on Forage Utilization: Effects of Fertility Levels and Stage of Maturity on Forage Nutritive Value , 1964 .

[14]  C. Cardona,et al.  Trends in biotechnological production of fuel ethanol from different feedstocks. , 2008, Bioresource technology.

[15]  L. Hongqiang,et al.  Detoxification of steam-exploded corn straw produced by an industrial-scale reactor , 2008 .

[16]  M. Ballesteros,et al.  Pretreatment technologies for an efficient bioethanol production process based on enzymatic hydrolysis: A review. , 2010, Bioresource technology.

[17]  S. Abiola,et al.  The utilization of alkali-treated melon husk by broilers. , 2002, Bioresource technology.

[18]  H. Verachtert,et al.  Growth of higher fungi on wheat straw and their impact on the digestibility of the substrate , 2004, Applied Microbiology and Biotechnology.

[19]  Aline Zorzetto Lopes Gonçalves,et al.  Enzyme production by solid-state fermentation: Application to animal nutrition , 2008 .

[20]  Ángel T. Martínez,et al.  Laccase detoxification of steam-exploded wheat straw for second generation bioethanol. , 2009, Bioresource technology.

[21]  K. Beauchemin,et al.  Exogenous enzymes added to untreated or ammoniated rice straw : Effects on in vitro fermentation characteristics and degradability , 2006 .

[22]  L. Haitao,et al.  Effect of fat type and lysophosphatidylcholine addition to broiler diets on performance, apparent digestibility of fatty acids, and apparent metabolizable energy content , 2011 .

[23]  Ye Sun,et al.  Hydrolysis of lignocellulosic materials for ethanol production: a review. , 2002, Bioresource technology.

[24]  P. V. Soest,et al.  Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. , 1991, Journal of dairy science.

[25]  Velmurugu Ravindran,et al.  Influence of whole wheat inclusion and xylanase supplementation on the performance, digestive tract measurements and carcass characteristics of broiler chickens , 2004 .

[26]  C. Nyachoti,et al.  Degradation of cell wall polysaccharides by combinations of carbohydrase enzymes and their effect on nutrient utilization and broiler chicken performance. , 2005, Poultry science.

[27]  Caixia Wan,et al.  Microbial delignification of corn stover by Ceriporiopsis subvermispora for improving cellulose digestibility , 2010 .

[28]  R. R. Marquardt,et al.  Use of enzymes to improve nutrient availability in poultry feedstuffs , 1996 .

[29]  Rui M. F. Bezerra,et al.  Effect of enzyme extracts isolated from white-rot fungi on chemical composition and in vitro digestibility of wheat straw , 2008 .

[30]  Yanbo Wang,et al.  Effect of probiotic on growth performance and digestive enzyme activity of Arbor Acres broilers. , 2010, Research in veterinary science.

[31]  K. Hachmeister,et al.  Tempeh: a mold-modified indigenous fermented food made from soybeans and/or cereal grains. , 1993, Critical reviews in microbiology.

[32]  P. H. Robinson,et al.  Influence of exogenous enzymes on nutrient digestibility, extent of ruminal fermentation as well as milk production and composition in dairy cows , 2009 .

[33]  Bärbel Hahn-Hägerdal,et al.  Fermentation of lignocellulosic hydrolysates. I: inhibition and detoxification , 2000 .

[34]  G Cardinale,et al.  Processing cereal straws by steam explosion in a pilot plant to enhance digestibility in ruminants. , 2008, Bioresource technology.

[35]  Jianxin Liu,et al.  Optimization of steam treatment as a method for upgrading rice straw as feeds , 1999 .

[36]  Claus Felby,et al.  Cell-wall structural changes in wheat straw pretreated for bioethanol production , 2008, Biotechnology for biofuels.

[37]  L. Olsson,et al.  Fermentation of lignocellulosic hydrolysates for ethanol production. , 1996 .

[38]  F. Guan,et al.  Production and characterization of cellulolytic enzymes from the thermoacidophilic fungal Aspergillus terreus M11 under solid-state cultivation of corn stover. , 2008, Bioresource technology.