Mitochondrial Inner Membrane Bound Pet1402 Protein Is Rapidly Imported into Mitochondria and Affects the Integrity of the Cytochrome Oxidase and Ubiquinol-Cytochrome c Oxidoreductase Complexes

In vitro synthesized Pet1402 precursor protein is very rapidly and efficiently imported into isolated mitochondria. The import depends on a membrane potential and functional mtHsp70. The mitochondrial targeting sequence of the Pet1402 precursor protein is removed by the matrix processing peptidase MPP and the mature protein is firmly embedded in the inner mitochondrial membrane. The Pet1402 protein is required for the integrity of the cytochrome oxidase and ubiquinol-cytochrome c oxidoreductase complexes.

[1]  W. Neupert,et al.  Insertion into the mitochondrial inner membrane of a polytopic protein, the nuclear‐encoded Oxa1p , 1997, The EMBO journal.

[2]  E. Pratje,et al.  A mutation in cytochrome oxidase subunit 2 restores respiration of the mutant pet ts1402 , 1997, Current Genetics.

[3]  M. Bauer,et al.  Import of carrier proteins into the mitochondrial inner membrane mediated by Tim22 , 1996, Nature.

[4]  L. Grivell,et al.  Promotion of Mitochondrial Membrane Complex Assembly by a Proteolytically Inactive Yeast Lon , 1996, Science.

[5]  N. Pfanner,et al.  The preprotein translocase of the inner mitochondrial membrane: evolutionary conservation of targeting and assembly of Tim17. , 1996, Journal of molecular biology.

[6]  L. Grivell,et al.  Three genes for mitochondrial proteins suppress null-mutations in both Afg3 and Rca1 when over-expressed , 1996, Current Genetics.

[7]  S. Papa,et al.  The Saccharomyces cerevisiae OXA1 gene is required for the correct assembly of cytochrome c oxidase and oligomycin‐sensitive ATP synthase , 1996, FEBS letters.

[8]  W. Neupert,et al.  Mechanisms of protein import across the mitochondrial outer membrane. , 1996, Trends in cell biology.

[9]  A. Tzagoloff,et al.  Mutations in RCA1 and AFG3 inhibit F1‐ATPase assembly in Saccharomyces cerevisiae , 1995, FEBS letters.

[10]  P. Slonimski,et al.  Cloning of a human gene involved in cytochrome oxidase assembly by functional complementation of an oxa1- mutation in Saccharomyces cerevisiae. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[11]  J. Jang,et al.  A new member of a family of ATPases is essential for assembly of mitochondrial respiratory chain and ATP synthetase complexes in Saccharomyces cerevisiae. , 1994, The Journal of biological chemistry.

[12]  P. Slonimski,et al.  OXA1, a Saccharomyces cerevisiae nuclear gene whose sequence is conserved from prokaryotes to eukaryotes controls cytochrome oxidase biogenesis. , 1994, Journal of molecular biology.

[13]  T. Langer,et al.  A dual role for mitochondrial heat shock protein 70 in membrane translocation of preproteins , 1993, The Journal of cell biology.

[14]  E. Craig,et al.  Presequence and mature part of preproteins strongly influence the dependence of mitochondrial protein import on heat shock protein 70 in the matrix , 1993, The Journal of cell biology.

[15]  H. Schägger,et al.  Blue native electrophoresis for isolation of membrane protein complexes in enzymatically active form. , 1991, Analytical biochemistry.

[16]  Y. Fujiki,et al.  Isolation of intracellular membranes by means of sodium carbonate treatment: application to endoplasmic reticulum , 1982, The Journal of cell biology.

[17]  M. Douglas,et al.  A neutral metallo endoprotease involved in the processing of an F1-ATPase subunit precursor in mitochondria. , 1982, The Journal of biological chemistry.

[18]  R. O. Poyton,et al.  Cytochrome c oxidase from bakers' yeast. I. Isolation and properties. , 1973, The Journal of biological chemistry.

[19]  L. Grivell.  Nucleo-mitochondrial interactions in mitochondrial gene expression. , 1995, Critical reviews in biochemistry and molecular biology.