THE EXTRACTION OF VALUABLE COMPOUNDS FROM STRAW DEGRADED BY PLEUROTUS OSTREATUS

This work aims to the recovery of lignocellulosic waste in an environmentally friendly process, as an alternative to the energyintensive technologies: steam explosion, subcritical and/or supercritical water treatment, gasification through pyrolisis, etc. A study was made to optimize the extraction conditions of potentially valuable compounds in straw degraded by the fungus Pleurotus ostreatus. The effects of solvent nature, temperature and extraction time were quantified by material balances with a special view to the extracts obtained. Confirmation of the effectiveness of the operations was done by spectrophotometric, HPLC and LC-MS analyses. Following this study, a technology localized to the farm was conceived, requiring few craftsmanship and no special utilities, to obtain a semi-product for further processing. A centralized technology could be also taken into account to process the straw by direct extraction with hot solvents, in order to obtain products yields three times higher than in the case of the aqueous extraction followed by solvent extraction at 20 o C.

[1]  S. Duff,et al.  Pretreatment and enzymatic hydrolysis of recovered fibre for ethanol production. , 2010, Bioresource technology.

[2]  Rui M. F. Bezerra,et al.  Modification of wheat straw lignin by solid state fermentation with white-rot fungi. , 2009, Bioresource technology.

[3]  Nicolaus Dahmen,et al.  Cost estimate for biosynfuel production via biosyncrude gasification , 2009 .

[4]  B. Mishra,et al.  Lignocellulolytic enzyme production from submerged fermentation of paddy straw , 2007, Indian Journal of Microbiology.

[5]  M. Bailey.  A note on the use of dinitrosalicylic acid for determining the products of enzymatic reactions , 1988, Applied Microbiology and Biotechnology.

[6]  E. Agosin,et al.  Solid-state fermentation, lignin degradation and resulting digestibility of wheat straw fermented by selected white-rot fungi , 1985, Applied Microbiology and Biotechnology.

[7]  J. Gressel,et al.  Biodegradation of wheat straw lignocarbohydrate complexes (LCC) , 1981, European journal of applied microbiology and biotechnology.

[8]  Q. Nguyen,et al.  Microbial pretreatment of biomass , 2003, Applied biochemistry and biotechnology.

[9]  H. Fölster,et al.  Comparison between colorimetric and titration methods for organic carbon determination in acidic soils , 2002 .

[10]  V. Bisaria,et al.  Effect of seed culture on solid-state bioconversion of wheat straw by Phanerochaete chrysosporium for animal feed production. , 2002, Journal of bioscience and bioengineering.

[11]  R. Lamuela-Raventós,et al.  Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent , 1999 .

[12]  A. Ball,et al.  Chemical characterization and spectroscopic analysis of the solubilization products from wheat straw produced by Streptomyces strains grown in solid-state fermentation. , 1997, Microbiology.

[13]  A. Bjerre,et al.  Pretreatment of wheat straw using combined wet oxidation and alkaline hydrolysis resulting in convertible cellulose and hemicellulose , 2000, Biotechnology and bioengineering.

[14]  A. Sethuraman,et al.  Microbial Delignification with White Rot Fungi Improves Forage Digestibility , 1993, Applied and environmental microbiology.

[15]  Yitzhak Hadar,et al.  Biodegradation of lignocellulosic agricultural wastes by Pleurotus ostreatus , 1993 .

[16]  S. L. Fales,et al.  Chemical composition and biodegradability of crop residues colonized by white-rot fungi , 1992 .

[17]  Ángel T. Martínez,et al.  Chemical transformation of wheat straw constituents after solid-state fermentation with selected lignocellulose-degrading fungi , 1991 .

[18]  V. Bisaria,et al.  An integrated approach to utilization of agro-residues through Pleurotus cultivation , 1987 .

[19]  R. Crawford,et al.  Microbial degradation of lignin , 1979 .