Physiological roles of endogenous nitric oxide in lymphatic pump activity of rat mesentery in vivo.

Physiological roles of endogenous nitric oxide (NO) in the lymphatic pump activity of rat mesenteries in vivo were evaluated using an intravital video microscope system. Changes in the pumping frequency (F), the end diastolic diameter (EDD), and the end systolic diameter (ESD) of the mesenteric lymph microvessels were measured with the microscope system and then the pump flow index (PFI) was calculated. A 15-min superfusion of 30 microM N(omega)-nitro-L-arginine methyl ester (L-NAME) in the mesenteries caused significant increases of F and PFI and a significant decrease of the EDD and ESD. Simultaneous superfusion of 1 mM L-arginine with 30 microM L-NAME produced a significant reversal of the L-NAME-mediated increase of F and decrease of ESD. A 15-min superfusion of 100 microM aminoguanidine caused no significant effects on F, EDD, and ESD of the mesenteric lymph vessels in vivo. These findings suggest that endogenous NO has physiologically modulated the lymphatic pump activity in rat mesentery in vivo and that the production and release of NO may be mediated by constitutive NO synthase but not by inducible NO synthase.

[1]  R. Elias,et al.  Is endothelium necessary for transmural pressure-induced contractions of bovine truncal lymphatics? , 1992, Microvascular research.

[2]  T. Ohhashi,et al.  Acetylcholine-induced release of endothelium-derived relaxing factor from lymphatic endothelial cells. , 1991, The American journal of physiology.

[3]  D. J. Wolff,et al.  Aminoguanidine is an isoform-selective, mechanism-based inactivator of nitric oxide synthase. , 1995, Archives of biochemistry and biophysics.

[4]  M. Ferguson,et al.  Nitric oxide and endothelium-dependent relaxation in tracheobronchial lymph vessels. , 1994, Microvascular research.

[5]  T. Campbell,et al.  Intrinsic contractility of lymphatics in sheep and in dogs. , 1973, Quarterly journal of experimental physiology and cognate medical sciences.

[6]  J Eisenhoffer,et al.  Evidence that the L-arginine pathway plays a role in the regulation of pumping activity in bovine mesenteric lymphatic vessels. , 1995, Microvascular research.

[7]  T. Ohhashi,et al.  Effects of acetylcholine on spontaneous contractions in isolated bovine mesenteric lymphatics. , 1993, The American journal of physiology.

[8]  T. Ohhashi,et al.  Active and passive mechanical characteristics of bovine mesenteric lymphatics. , 1980, The American journal of physiology.

[9]  J. Benoit.  Effects of alpha-adrenergic stimuli on mesenteric collecting lymphatics in the rat. , 1997, The American journal of physiology.

[10]  D. V. van Helden,et al.  Endothelium‐dependent modulation of pacemaking in lymphatic vessels of the guinea‐pig mesentery. , 1996, The Journal of physiology.

[11]  J. Hall,et al.  Intrinsic rhythmic propulsion of lymph in the unanaesthetized sheep. , 1965, The Journal of physiology.

[12]  P. Kubes,et al.  Inhibition of nitric oxide production. Mechanisms of vascular albumin leakage. , 1993, Circulation research.

[13]  A. Koller,et al.  Myogenic Responses of Isolated Lymphatics: Modulation by Endothelium , 1997, Microcirculation.

[14]  G. Thurston,et al.  Inhibition of nitric oxide synthesis increases venular permeability and alters endothelial actin cytoskeleton. , 1998, American journal of physiology. Heart and circulatory physiology.

[15]  T. Ohhashi,et al.  Macrophage-induced nitric oxide and prostanoid dependent relaxation of arterial smooth muscles. , 1997, Canadian journal of physiology and pharmacology.

[16]  L. Leak,et al.  Nitric oxide production by lymphatic endothelial cells in vitro. , 1995, Biochemical and biophysical research communications.

[17]  T. Ohhashi,et al.  Immunolocalization of Nitric Oxide Synthase and VEGF Receptors in Cultured Lymphatic Endothelial Cells , 1999, Microcirculation.

[18]  B. Zweifach,et al.  Contractile stimuli in collecting lymph vessels. , 1977, The American journal of physiology.

[19]  M. Reguzzoni,et al.  Endothelin and nitric oxide synthase in lymphatic endothelial cells: Immunolocalization in vivo and in vitro , 1997, The Anatomical record.

[20]  H. Granger,et al.  Characterization of intact mesenteric lymphatic pump and its responsiveness to acute edemagenic stress. , 1989, The American journal of physiology.

[21]  Gabor Kaley,et al.  Regulation of the vasomotor activity of lymph microvessels by nitric oxide and prostaglandins. , 1998, American journal of physiology. Regulatory, integrative and comparative physiology.

[22]  T. Aw,et al.  Microvascular responses to inhibition of nitric oxide production. Role of active oxidants. , 1995, Circulation research.

[23]  B. Zweifach,et al.  Micromanipulation of pressure in terminal lymphatics in the mesentery. , 1975, The American journal of physiology.

[24]  I. Roddie,et al.  Spontaneous activity in isolated bovine mesenteric lymphatics , 1973, The Journal of physiology.