Combining multiphoton and CARS microscopy for skin imaging

We present combined coherent anti Stokes Raman scattering and two-photon excited fluorescence microscopy, for label-free in vitro and in vivo imaging of skin with both chemical discrimination and subcellular resolution. In particular, we show how CARS microscopy is utilized to image lipid-rich structures inside the skin, preparing for a combined multiphoton and CARS imaging modality for biomedical research and skin imaging. As a further potential application the detection of topically applied oil on the skin is presented.

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