The influence of different fixatives and fixation methods on the ultrastructure of rat kidney proximal tubule cells. I. Comparison of different perfusion fixation methods and of glutaraldehyde, formaldehyde and osmium tetroxide fixatives.

The ultrastructure of rat kidney proximal tubule cells was compared following fixation by different methods (microperfusion of single tubules, different forms of vascular perfusion, dripping of the fixative on the kidney surface). A comparison was also made between glutaraldehyde, formaldehyde and osmium tetroxide fixatives. The rat kidney cortex could be uniformely fixed by vascular perfusion with both aldehyde and osmium tetroxide fixatives. Characteristic ultrastructural differences were observed between cells fixed with the different fixation methods and with the different fixatives. The observations demonstrate a great sensitivity of the proximal tubule cells to different conditions of fixation and illustrate the necessity of considering this when analyzing the ultrastructural organization of these cells.

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