Carotenoid profiling of tropical root crop chemotypes from Vanuatu, South Pacific

Tropical root and tuber crops are staple foods in developing countries where they are the main source of carbohydrates and a potential source of secondary metabolites such as carotenoids. Enhancing carotenoid content has considerable potential for improving human nutrition in these areas. Except for cassava and sweet potato, carotenoid content has not been evaluated in large germplasm collections. We describe a simple HPLC-DAD-based protocol for the reliable characterization of carotenoids in root crops. Less than 5% variation in carotenoid content was found among samples collected from tubers of the same plant or from clones of the same genotype. Carotenoids were evaluated in 153 accessions representing 10 different species. Each species had a specific profile of carotenoids, and accessions of the same species differed greatly in their individual content. The database assembled during this study represents a useful tool for nutritionists and breeders working on biofortification of root crops with carotenoids.

[1]  D. Snodderly,et al.  Predictors of optical density of lutein and zeaxanthin in retinas of older women in the Carotenoids in Age-Related Eye Disease Study, an ancillary study of the Women's Health Initiative. , 2006, The American journal of clinical nutrition.

[2]  J. Mayer,et al.  Genetic potential and stability of carotene content in cassava roots , 2004, Euphytica.

[3]  C. Rice-Evans,et al.  Antioxidant activities of carotenes and xanthophylls , 1996, FEBS letters.

[4]  D. Rodriguez-Amaya,et al.  HarvestPlus Handbook for Carotenoid Analysis. , 2004 .

[5]  G. C. Yencho,et al.  Antioxidant activities, phenolic and β-carotene contents of sweet potato genotypes with varying flesh colours , 2007 .

[6]  J. Humphries,et al.  Carotenoid and mineral content of Micronesian giant swamp taro (Cyrtosperma) cultivars , 2008 .

[7]  J. Bradbury,et al.  Book Review: Chemistry of Tropical Root Crops: Significance for Nutrition and Agriculture in the Pacific , 1989 .

[8]  T. Goodwin.  Chemistry and biochemistry of plant pigments , 1976 .

[9]  A. Pérez-Gálvez,et al.  Structure-reactivity relationship in the oxidation of carotenoid pigments of the pepper (Capsicum annuum L.). , 2001, Journal of agricultural and food chemistry.

[10]  W. Stahl,et al.  Vitamins E and C, beta-carotene, and other carotenoids as antioxidants. , 1995, The American journal of clinical nutrition.

[11]  J. Buttriss,et al.  A review of the epidemiological evidence for the ‘antioxidant hypothesis’ , 2004, Public Health Nutrition.

[12]  P M Bramley,et al.  Application of high‐performance liquid chromatography with photodiode array detection to the metabolic profiling of plant isoprenoids , 2000 .

[13]  K. Scott,et al.  Pro-vitamin A carotenoid conversion factors: retinol equivalents - fact or fiction? , 2000 .

[14]  Jimaima V. Lako,et al.  Phytochemical flavonols, carotenoids and the antioxidant properties of a wide selection of Fijian fruit, vegetables and other readily available foods , 2007 .

[15]  E. Carey,et al.  Influence of Age, Farming Site, and Boiling on Pro-Vitamin A Content in Sweet Potato (Ipomoea batatas(L.) Lam.) Storage Roots , 1998 .

[16]  Chien-Chun Huang,et al.  Comparison of Taiwan paddy- and upland-cultivated taro (Colocasia esculenta L.) cultivars for nutritive values , 2007 .

[17]  T. Nagata,et al.  HPLC Determination of β-Carotene Content of Sweet Potato Cultivars and Its Relationship with Color Values , 1993 .

[18]  W. Stahl,et al.  Bioactivity and protective effects of natural carotenoids. , 2005, Biochimica et biophysica acta.

[19]  P. Nestel,et al.  (cid:1) -Carotene–rich orange-fleshed sweet potato improves the vitamin A status of primary school children assessed with the modified-relative-dose-response test 1–3 , 2005 .

[20]  M. Dennis,et al.  HPLC analysis of carotenoids in orange juice , 1999 .

[21]  E. Carey,et al.  Potential of sweet potato in reducing vitamin A deficiency in Africa. , 2000 .

[22]  A. J. Meléndez-Martínez,et al.  Rapid assessment of vitamin A activity through objective color measurements for the quality control of orange juices with diverse carotenoid profiles. , 2007, Journal of agricultural and food chemistry.