The objective of this study was to explore the application of blended teaching in medical biochemistry and molecular biology course for clinical medical students. Using the Zhihuishu platform and the Test Database of Chinese Medical Education by People's Medical Publishing House as online educational materials, combined with traditional place-based classroom methods, to explore the transformation of teaching mode which is suitable for medical education and teaching characteristics in the information technology era. It provides experience for the teaching reform of biochemistry and molecular biology course, and will help to improve teaching effect, improve teaching theory and construct new teaching models.