Effects of reproductive stage and 11-ketotestosterone on LPL mRNA levels in the ovary of the shortfinned eel

To understand the dynamics of lipid uptake into the ovary and the potential role that lipoprotein lipase plays in this event, changes in LPL transcript abundance during oogenesis were measured in both wild-caught and pituitary homogenate-induced artificially maturing eels. Also, the effects of 11-ketotestosterone (11-KT) on LPL mRNA levels were investigated in vivo and in vitro. Normalized ovarian LPL transcript abundance increased as oogenesis advanced, and it rose particularly rapidly during midvitellogenesis, corresponding to pronounced increases in ovarian lipid deposits and LPL activity. Furthermore, LPL mRNA levels were dramatically increased following 11-KT treatment in vivo, findings that were reinforced as trends in ovarian tissue incubated in vitro. Ovarian LPL appears to be directly involved in the uptake of lipids into the eel ovary, an involvement that appears to be controlled, at least in part, by the androgen 11-KT.

[1]  H. Matsubara,et al.  Effects of reproductive stage, GH, and 11-ketotestosterone on expression of growth differentiation factor-9 in the ovary of the eel, Anguilla australis. , 2010, Reproduction.

[2]  M. Rise,et al.  Stimulation of growth and changes in the hepatic transcriptome by 17beta-estradiol in the yellow perch (Perca flavescens). , 2009, Physiological genomics.

[3]  J. Peinado-Onsurbe,et al.  Lipoprotein lipase (LPL) is highly expressed and active in the ovary of European sea bass (Dicentrarchus labrax L.), during gonadal development. , 2008, Comparative biochemistry and physiology. Part A, Molecular & integrative physiology.

[4]  Takeshi Yamamoto,et al.  Effect of n-3 and n-6 fatty acids in broodstock diet on reproduction and fatty acid composition of broodstock and eggs in the Japanese eel Anguilla japonica , 2007 .

[5]  P. Lokman,et al.  11-Ketotestosterone and IGF-I increase the size of previtellogenic oocytes from shortfinned eel, Anguilla australis, in vitro. , 2007, Reproduction.

[6]  A. Albalat,et al.  Regulation of lipoprotein lipase activity in rainbow trout (Oncorhynchus mykiss) tissues. , 2006, General and comparative endocrinology.

[7]  S. Ando,et al.  A unique lipoprotein profile found in the plasma of cultured Japanese eelAnguilla japonica: very low density lipoprotein, but not high density lipoprotein, is the main component of plasma , 1996, Fish Physiology and Biochemistry.

[8]  J. Kroll,et al.  FASTING AND STARVATION , 2005 .

[9]  P. Lokman,et al.  Gonad histology and plasma steroid profiles in wild New Zealand freshwater eels (Anguilla dieffenbachii and A. australis) before and at the onset of the natural spawning migration. II. Males , 1998, Fish Physiology and Biochemistry.

[10]  M. D. Wiegand.  Composition, accumulation and utilization of yolk lipids in teleost fish , 1996, Reviews in Fish Biology and Fisheries.

[11]  S. Dufour,et al.  Positive feedback control by the gonads on gonadotropin (GTH) and gonadoliberin (GnRH) levels in experimentally matured female silver eels,Anguilla anguilla , 1989, Fish Physiology and Biochemistry.

[12]  D. Tocher.  Metabolism and Functions of Lipids and Fatty Acids in Teleost Fish , 2003 .

[13]  Tohru Kobayashi,et al.  Molecular cloning and expression during spermatogenesis of a cDNA encoding testicular 11β‐hydroxylase (P45011β) in rainbow trout (Oncorhynchus mykiss) , 2002 .

[14]  G. Olivecrona,et al.  Lipoprotein lipase from rainbow trout differs in several respects from the enzyme in mammals. , 2002, Gene.

[15]  C. Randall,et al.  Molecular Characterization of Putative Yolk Processing Enzymes and Their Expression During Oogenesis and Embryogenesis in Rainbow Trout (Oncorhynchus mykiss)1 , 2001, Biology of reproduction.

[16]  P. Lokman,et al.  11-Ketotestosterone induces silvering-related changes in immature female short-finned eels, Anguilla australis. , 2001, Comparative biochemistry and physiology. Part A, Molecular & integrative physiology.

[17]  Y. Takeuchi,et al.  Quality of eggs and spermatozoa of rainbow trout fed an n-3 essential fatty acid-deficient diet and its effects on the lipid and fatty acid components of eggs, semen and livers. , 2001 .

[18]  I. Goldberg,et al.  Caloric and vitamin distribution by LpL 4. Synthesis and processing of lipases 5. Lipoproteins and regulation of the lipolysis reaction 6. Apolipoproteins 7. Non-enzymatic actions of lipoprotein lipase 8. Structure-function analysis of lipoprotein lipase 9.Genetic variation in lipoprotein lipase 10. , 2002 .

[19]  G. Young,et al.  Changes in steroid hormone profiles and ovarian histology during salmon pituitary-induced vitellogenesis and ovulation in female New Zealand longfinned eels, Anguilla dieffenbachii gray. , 2001, The Journal of experimental zoology.

[20]  H. Matsubara,et al.  Cloning of 17beta-hydroxysteroid dehydrogenase-I cDNAs from Japanese eel ovary. , 2000, Biochemical and biophysical research communications.

[21]  P. Lokman,et al.  Induced spawning and early ontogeny of New Zealand freshwater eels {Anguilla dieffenbachii and A. australis) , 2000 .

[22]  D. Illingworth,et al.  Lipoproteins in Health and Diseases , 1999 .

[23]  Y. Kao,et al.  REGULATION OF METAMORPHOSIS-ASSOCIATED CHANGES IN THE LIPID METABOLISM OF SELECTED VERTEBRATES , 1998 .

[24]  K. Reitan,et al.  The significance of lipids at early stages of marine fish: a review , 1997 .

[25]  K. Yamauchi,et al.  Changes in serum steroid hormones and steroidogenic ability of ovarian follicles during artificial maturation of cultivated Japanese eel, Anguilla japonica , 1995 .

[26]  I. Navarro,et al.  Chapter 17 Fasting and starvation , 1995 .

[27]  H. Kuhl,et al.  Short- and Long-Term Effects on Lipid Metabolism of Oral Contraceptives Containing 30 µg Ethinylestradiol and 150 µg Desogestrel or 3-Keto-Desogestrel , 1995 .

[28]  P. Björntorp,et al.  Assimilation and mobilization of triglycerides in subcutaneous abdominal and femoral adipose tissue in vivo in men: effects of androgens. , 1995, The Journal of clinical endocrinology and metabolism.

[29]  J. Gimble,et al.  Lipoprotein lipase gene expression: physiological regulators at the transcriptional and post-transcriptional level. , 1993, Biochimica et biophysica acta.

[30]  H. Brewer,et al.  Human lipoprotein lipase: the loop covering the catalytic site is essential for interaction with lipid substrates. , 1992, The Journal of biological chemistry.

[31]  H. Brewer,et al.  Human lipoprotein lipase. Analysis of the catalytic triad by site-directed mutagenesis of Ser-132, Asp-156, and His-241. , 1992, The Journal of biological chemistry.

[32]  T. Miura,et al.  Hormonal induction of all stages of spermatogenesis in vitro in the male Japanese eel (Anguilla japonica). , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[33]  R. S. Cone,et al.  The Need to Reconsider the Use of Condition Indices in Fishery Science , 1989 .

[34]  T. Olivecrona,et al.  Lipoprotein lipase in myocytes and capillary endothelium of heart: immunocytochemical study. , 1989, The American journal of physiology.

[35]  P. J. Babin,et al.  Plasma lipoproteins in fish. , 1989, Journal of lipid research.

[36]  D. Covés,et al.  The characteristics of sea bass (Dicentrarchus labrax) eggs : description, biochemical composition and hatching performances , 1988 .

[37]  S. Dufour,et al.  Stimulation of gonadotropin release and of ovarian development, by the administration of a gonadoliberin agonist and of dopamine antagonists, in female silver eel pretreated with estradiol. , 1988, General and comparative endocrinology.

[38]  G. C. Packard,et al.  The Misuse of Ratios, Indices, and Percentages in Ecophysiological Research , 1988, Physiological Zoology.

[39]  T. Kirchgessner,et al.  Human lipoprotein lipase complementary DNA sequence. , 1987, Science.

[40]  E. Skinner,et al.  Changes in plasma lipoproteins and tissue lipoprotein lipase and salt-resistant lipase activities during spawning in the rainbow trout (Salmo gairdnerii R.). , 1987, Comparative biochemistry and physiology. B, Comparative biochemistry.

[41]  Takeshi Watanabe.  Lipid nutrition in fish , 1982 .

[42]  P. Nilsson-ehle,et al.  Lipolytic enzymes and plasma lipoprotein metabolism. , 1980, Annual review of biochemistry.

[43]  R. Kaur,et al.  Morphology of ovarian changes during the reproductive cycle of the fish, Mystus tengara (Ham.). , 1975, Acta anatomica.

[44]  M. Johansson-Sjöbeck,et al.  Metabolic and hematological effects of starvation in the European eel, Anguilla anguilla L. I. Carbohydrate, lipid, protein and inorganic ion metabolism. , 1975, Comparative biochemistry and physiology. A, Comparative physiology.

[45]  G. Dave,et al.  Metabolic and hematological studies on the yellow and silver phases of the European eel, Anguilla anguilla L. III. Hematology. , 1974, Comparative biochemistry and physiology. B, Comparative biochemistry.

[46]  G. Dave,et al.  Metabolic and hematological studies on the yellow and silver phases of the European eel, Anguilla anguilla L. I. Carbohydrate, lipid, protein and inorganic ion metabolism. , 1974, Comparative biochemistry and physiology. B, Comparative biochemistry.

[47]  J. Folch,et al.  A simple method for the isolation and purification of total lipides from animal tissues. , 1957, The Journal of biological chemistry.

[48]  E. Cren,et al.  THE LENGTH-WEIGHT RELATIONSHIP AND SEASONAL CYCLE IN GONAD WEIGHT AND CONDITION IN THE PERCH , 2022 .