Identification of two distinct regions within the adenovirus minimal origin of replication that are required for adenovirus type 4 DNA replication in vitro

The adenovirus type 4 origins of replication are located at each end of the linear, protein-linked viral DNA molecule and consist of the terminal 18 bp of the viral genome. The sequence of the first 8 bp of the viral genome varies among different adenovirus serotypes, but the sequence from bp 9 to 18 is conserved in all human serotypes, suggesting that it may be of critical importance to origin function. Using an in vitro system in which purified fractions or crude extracts of adenovirus type 4-infected HeLa cells can support initiation and elongation on linearized plasmid templates containing cloned origin sequences, we examined the effect of single base changes in positions 9 to 18 of the adenovirus origin on DNA replication in vitro. Changes in positions 12 to 16 have little effect, whereas alterations at positions 9, 10, 11, 17, and 18 all reduce the efficiency of initiation of DNA replication by between 50 and 90%. Our results show that the region from bp 9 to 18 contains two sets of bases essential for DNA replication which are separated by 5 bp in which single base changes can be accommodated. The likely role of the region from bp 9 to 18 as containing the recognition sequence for a DNA-protein interaction essential for viral DNA replication is discussed.

[1]  R. Hay,et al.  Replication of adenovirus type 4 DNA by a purified fraction from infected cells. , 1991, Nucleic acids research.

[2]  R. Hay,et al.  Interactions between the adenovirus type 2 DNA polymerase and the DNA binding domain of nuclear factor I. , 1990, The New biologist.

[3]  C. Verrijzer,et al.  Transcription factors NFI and NFIII/oct-1 function independently, employing different mechanisms to enhance adenovirus DNA replication , 1990, Journal of virology.

[4]  C. Verrijzer,et al.  The DNA binding domain (POU domain) of transcription factor oct‐1 suffices for stimulation of DNA replication. , 1990, The EMBO journal.

[5]  R. Cortese,et al.  Amino‐terminal domain of NF1 binds to DNA as a dimer and activates adenovirus DNA replication. , 1990, The EMBO journal.

[6]  P. C. van der Vliet,et al.  Adenovirus DNA-binding protein forms a multimeric protein complex with double-stranded DNA and enhances binding of nuclear factor I , 1990, Journal of virology.

[7]  E. A. O'neill,et al.  The proline-rich transcriptional activator of CTF/NF-I is distinct from the replication and DNA binding domain , 1989, Cell.

[8]  R. Hay,et al.  Co‐operative interactions between NFI and the adenovirus DNA binding protein at the adenovirus origin of replication. , 1989, The EMBO journal.

[9]  R. Hay,et al.  Recognition mechanisms in the synthesis of animal virus DNA. , 1989, The Biochemical journal.

[10]  Nicolas Mermod,et al.  A family of human CCAAT-box-binding proteins active in transcription and DNA replication: cloning and expression of multiple cDNAs , 1988, Nature.

[11]  J. Hurwitz,et al.  Initiation of adenovirus DNA replication. II. Structural requirements using synthetic oligonucleotide adenovirus templates. , 1988, The Journal of biological chemistry.

[12]  J. Hurwitz,et al.  Initiation of adenovirus DNA replication. I. Mechanism of action of a host protein required for replication of adenovirus DNA templates devoid of the terminal protein. , 1988, The Journal of biological chemistry.

[13]  R. Hay,et al.  DNA sequences required for the initiation of adenovirus type 4 DNA replication in vitro. , 1988, Journal of molecular biology.

[14]  E. A. O'neill,et al.  Purification and characterization of nuclear factor III (origin recognition protein C), a sequence-specific DNA binding protein required for efficient initiation of adenovirus DNA replication. , 1988, The Journal of biological chemistry.

[15]  E. A. O'neill,et al.  Sequence-specific interactions between cellular DNA-binding proteins and the adenovirus origin of DNA replication , 1987, Molecular and cellular biology.

[16]  M. Ishino,et al.  Nucleotide sequence of the adenovirus type 40 inverted terminal repeat: close relation to that of adenovirus type 5. , 1987, Virology.

[17]  R. Wides,et al.  Adenovirus origin of DNA replication: sequence requirements for replication in vitro , 1987, Molecular and cellular biology.

[18]  G. Pruijn,et al.  Nuclear factor III, a novel sequence-specific DNA-binding protein from HeLa cells stimulating adenovirus DNA replication , 1986, Nature.

[19]  J. Hurwitz,et al.  Reconstruction of adenovirus replication origins with a human nuclear factor I binding site. , 1986, The Journal of biological chemistry.

[20]  E. Winnacker,et al.  A sensitive and rapid gel retention assay for nuclear factor I and other DNA-binding proteins in crude nuclear extracts. , 1986, Nucleic acids research.

[21]  Jacques H. van Boom,et al.  Adenovirus DNA replication in vitro: site-directed mutagenesis of the nuclear factor I binding site of the Ad2 origin , 1985, Nucleic Acids Res..

[22]  W. Driel,et al.  Recognition site of nuclear factor I, a sequence‐specific DNA‐binding protein from HeLa cells that stimulates adenovirus DNA replication. , 1985, The EMBO journal.

[23]  R. Hay.  The origin of adenovirus DNA replication: minimal DNA sequence requirement in vivo. , 1985, The EMBO journal.

[24]  J. Hurwitz,et al.  Protein-primed replication of plasmids containing the terminus of the adenovirus genome. II. Purification and characterization of a host protein required for the replication of DNA templates devoid of the terminal protein. , 1984, The Journal of biological chemistry.

[25]  N. Stow,et al.  Replication of adenovirus mini-chromosomes. , 1984, Journal of molecular biology.

[26]  F. Tamanoi,et al.  The origin of adenovirus DNA replication. , 1984, Current topics in microbiology and immunology.

[27]  M. Challberg,et al.  Template requirements for the initiation of adenovirus DNA replication. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[28]  J. Sussenbach,et al.  Specific binding of the adenovirus terminal protein precursor-DNA polymerase complex to the origin of DNA replication. , 1983, Nucleic acids research.

[29]  J. Hurwitz,et al.  Specific binding of a cellular DNA replication protein to the origin of replication of adenovirus DNA. , 1983, Proceedings of the National Academy of Sciences of the United States of America.

[30]  J. Hurwitz,et al.  Adenoviral protein-primed initiation of DNA chains in vitro. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[31]  R. Roberts,et al.  The nucleotide sequences at the termini of adenovirus-2 DNA. , 1979, Journal of molecular biology.

[32]  M. Challberg,et al.  Adenovirus DNA replication in vitro. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[33]  D. Rekosh,et al.  Identification of a protein linked to the ends of adenovirus DNA , 1977, Cell.

[34]  M. M. Bradford.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.

[35]  J. Williams.  Enhancement of adenovirus plaque formation on HeLa cells by magnesium chloride. , 1970, The Journal of general virology.