Genetic Polymorphism of GHR, LEP and MSTN Genes in Surti Goats from Organized Farm

Genetic studies pertaining to genes related to growth parameters can give idea about growth, meat as well as milk production performance of the animals in advance. Surti is considered as a dual purpose goat breed mainly of South Gujarat region. The aim of the present study was to know the genetic polymorphism in growth related genes, viz., growth hormone receptor (GHR), leptin (LEP) and myostatin (MSTN) through PCR-RFLP technique. The blood samples were collected from 62 Surti goats and DNA was extracted. Amplification of the genes was carried out using suitable primers. The PCR products of GHR, LEP and MSTN were digested with AvaII, HinfI and HaeIII enzymes, respectively. PCR products were found to be monomorphic on digestion.

[1]  U. Ramani,et al.  Genetic Polymorphism of Growth Hormone Gene Exon-4 in Surti and Mehsani Goats by PCR- RFLP , 2018, THE INDIAN JOURNAL OF VETERINARY SCIENCES AND BIOTECHNOLOGY.

[2]  X. An,et al.  Polymorphism identification in the goat MSTN gene and association analysis with growth traits. , 2018 .

[3]  Anurodh Sharma,et al.  Sequence characterization and genetic variability analysis of GHR, IGF1, and IGFBP-3 genes in nine Indian goat breeds , 2014 .

[4]  B. Mishra,et al.  Preliminary identification and characterisation of leptin gene polymorphism in Indian goats , 2014 .

[5]  Y. Ling,et al.  Polymorphisms of the myostatin gene (MSTN) and its relationship with growth traits in goat breeds. , 2013, Genetics and molecular research : GMR.

[6]  E. Ahmed,et al.  Genetic polymorphism of five genes associated with growth traits in goat , 2012 .

[7]  C. Supakorn.  The Important Candidate Genes in Goats - A Review , 2011 .

[8]  J. Kaur,et al.  Genetic variability of growth hormone gene and its association with growth traits in Sirohi breed of goat , 2011 .

[9]  X. An,et al.  Novel polymorphisms of goat growth hormone and growth hormone receptor genes and their effects on growth traits , 2011, Molecular Biology Reports.

[10]  Sudheer Kumar Singh,et al.  Characterization of Exon 2 and Intron 2 of Leptin Gene in Indian Goats , 2009, Animal biotechnology.

[11]  L. Han,et al.  Polymorphism of the growth hormone gene and its association with growth traits in Boer goat bucks. , 2009, Meat science.

[12]  A. Pandey,et al.  Single nucleotide polymorphism (SNP) in growth hormone gene of Jakhrana, a prominent milk goat breed in India , 2009 .

[13]  L. Zwierzchowski,et al.  A TG-repeat polymorphism in the 5 -noncoding region of the goat growth hormone receptor gene and search for its association with milk production traits , 2007 .

[14]  M. Pereirab,et al.  Association of milk traits with SSCP polymorphisms at the growth hormone gene in the Serrana goat , 2003 .

[15]  R. Rozen,et al.  A rapid procedure for extracting genomic DNA from leukocytes. , 1991, Nucleic acids research.